Browse > Article
http://dx.doi.org/10.3839/jabc.2020.050

Molecular analysis of peptide toxins secreted by various Pseudomonas tolaasii strains  

Yun, Yeong-Bae (Department of Environmental and Biological Chemistry, Chungbuk National University)
Kim, Young-Kee (Department of Environmental and Biological Chemistry, Chungbuk National University)
Publication Information
Journal of Applied Biological Chemistry / v.63, no.4, 2020 , pp. 387-392 More about this Journal
Abstract
Pseudomonas tolaasii is a pathogen causing brown blotch disease in cultivated mushrooms. In previous study, various strains of P. tolaasii were isolated from the mushrooms with disease symptoms and they were further divided into Ptα, Ptβ, and Ptγ subtypes according to the 16S rRNA gene analysis. To investigate the secretion of peptide toxins, tolaasin and its analog peptides, culture extracts of Pt group strains were analyzed by gel permeation chromatography. Those of Ptα subtype strains contained two chromatographic peaks, band A and B. Meanwhile, those of Ptβ and Ptγ subtype strains contained mainly band A component and a little of band B. Molecular weights of toxic peptides of culture extracts were measured by MALDI-TOF mass spectrometry. In Ptα subtype strains, the peptide compositions of band A and B were same including tolaasin I (1,987 Da), tolaasin II (1,943 Da), and its two analog peptides, 1,973 Da and 2,005 Da. The strains of Ptβ and Ptγ subtype secreted many components of MW 1,100-1,200 Da, but they did not synthesize any tolaasin-like peptides. These results suggest that the only Ptα subtype strains secrete tolaasin and its analog peptide toxins and the strains of Ptβ and Ptγ subtypes have different pathogenic characters causing brown blotch disease.
Keywords
Brown blotch disease; Mass spectroscopy; Peptide composition; Tolaasin;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Jourdan F, Lazzaroni S, Mendez BL, Lo Cantore P, de Julio M, Amodeo P, Iacobellis NS, Evidente A, Motta A (2003) A left-handed α-helix containing both L- and D-amino acids: the solution structure of the antimicrobial lipodepsipeptide tolaasin. Proteins 52: 534-543   DOI
2 Silva T, Claro B, Silva BFB, Vale N, Gomes P, Gomes MS, Funari SS, Teixeira J, Uhrikova D, Bastos M (2018) Unravelling a mechanism of action for a cecropin A-melittin hybrid antimicrobial peptide: the induced formation of multilamellar lipid stacks. Langmuir 34: 2158-2170   DOI
3 Nutkins JC, Mortishire-Smith RJ, Packman LC, Brodey CL, Rainey PB, Johnstone K, Williams DH (1991) Structure determination of tolaasin, an extracellular lipodepsipeptide produced by the mushroom pathogen Pseudomonas tolaasii Paine. J Am Chem Soc 113: 2621-2627   DOI
4 Bassarello C, Lazzaroni S, Bifulco G, Lo Cantore P, Iacobellis NS, Riccio R, Gomez-Paloma L, Evidente A (2004) Tolaasins A-E, five new lipodepsipeptides produced by Pseudomonas tolaasii. J Nat Prod 67: 811-816   DOI
5 Shirata A, Sugaya K, Takasugi M, Monde K (1995) Isolation and biological activity of toxins produced by a Japanese strain of Pseudomonas tolaasii, the pathogen of bacterial rot of cultivated oyster mushroom. Ann Phytopathol Soc Jpn 61: 493-502   DOI
6 Cho KH, Kim ST, Kim YK (2007) Purification of a pore-forming peptide toxin, tolaasin, produced by Pseudomonas tolaasii 6264. J Biochem Mol Biol 40: 113-118
7 Yun YB, Park SW, Cha JS, Kim YK (2013) Biological characterization of various strains of Pseudomonas tolaasii that causes brown blotch disease. J Korean Soc Appl Biol Chem 56: 41-45   DOI
8 Abou-Zeid MA (2012) Pathogenic variation in isolates of Pseudomonas causing the brown blotch cultivated mushroom, Agaricus bisporus. Braz J Microbiol 43: 1137-1146   DOI
9 Coraiola M, Lo Cantore P, Lazzaroni S, Evidente A, Iacobellis NS, Dalla Serra M (2006) WLIP and tolaasin I, lipodepsipeptides from Pseudomonas reactans and Pseudomonas tolaasii, permeabilise model membranes. Biochim Biophys Acta 1758: 1713-1722   DOI
10 Cho KH, Wang HS, Kim YK (2009) Temperature-dependent hemolytic activity of membrane pore-forming peptide toxin, tolaasin. J Pept Sci 16: 85-90   DOI
11 Soler-Rivas C, Jolivet S, Arpin N, Olivier JM, Wichers HJ (1999) Biochemical and physiological aspects of brown blotch disease of Agaricus bisporus. FEMS Microbiol Rev 23: 591-614   DOI
12 Yun YB (2020) Pathogenic characterization of various phage-resistant mutant strains induced from Pseudomonas tolaasii. Dissertation, Chungbuk National University
13 Nemergut DR, Costello EK, Hamady M, Lozupone C, Jiang L, Schmidt SK, Fierer N, Townsend AR, Cleveland CC, Stanish L, Knight R (2011) Global patterns in the biogeography of bacterial taxa. Environ Microbiol 13: 135-144   DOI
14 Gilbert JA, Steele JA, Caporaso JG, Steinbruck L, Reeder J, Temperton B, Huse S, McHardy AC, Knight R, Joint I, Somerfield P, Fuhrman JA, Field D (2012) Defining seasonal marine microbial community dynamics. ISME J 6: 298-308   DOI
15 Quince C, Lanzen A, Davenport RJ, Turnbaugh PJ (2011) Removing noise from pyrosequenced amplicons. BMC Bioinformatics 12: 38   DOI
16 Poretsky R, Rodriguez-R LM, Luo C, Tsementzi D, Konstantinidis KT (2014) Strengths and limitations of 16S rRNA gene amplicon sequencing in revealing temporal microbial community dynamics. PLoS ONE 9: e93827   DOI
17 Lee GS, Byun HS, Yoon KH, Lee JS, Choi KC, Jeung EB (2009) Dietary calcium and vitamin D2 supplementation with enhanced Lentinula edodes improves osteoporosis-like symptoms and induces duodenal and renal active calcium transport gene expression in mice. Eur J Nutr 48: 75-83   DOI
18 Zhang M, Huang J, Xie X, Holman CJ (2009) Dietary intakes of mushrooms and green tea combine to reduce the risk of breast cancer in Chinese women. Int J Cancer 124: 1404-1408   DOI
19 Annual Report for Production of Mushroom (2019) Agricultural information and statistics. Ministry of Agriculture and Forestry, Sejong, Korea