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http://dx.doi.org/10.3839/jabc.2020.015

Optimization of extraction conditions of flavonoid compounds from Thyme (Thymus vulgaris Libiatae)  

Park, Yunjin (Department of Food and Pharmaceutical Engineering & Institute of Basic Science, Hoseo University)
Lee, Jumi (Department of Food and Pharmaceutical Engineering & Institute of Basic Science, Hoseo University)
In, Man-Jin (Department of Chemical Engineering, Chungwoon University)
Chae, Hee Jeong (Department of Food and Pharmaceutical Engineering & Institute of Basic Science, Hoseo University)
Publication Information
Journal of Applied Biological Chemistry / v.63, no.1, 2020 , pp. 111-116 More about this Journal
Abstract
Total polyphenol contents, flavonoid contents, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activities of ethanol extracts of 20 herbal plants (sage, turmeric, mace, bayleaf, fenugreek, oregano, blackpepper, whitepepper, clove, marjoram, cinnamon, coriander, basil, dillseed, mustard, cadamon, thyme, celery, rosemary, cumin) were analyzed for the screening of high flavonoid-containing plant resource. Thyme extract, showing the highest flavonoid content and a high degree of antioxidant activity, was selected as a bioactive cosmetic material. The total polyphenols and flavonoids contents of thyme extracts were measured as 6.90 mg chlorogenic acid equivalent (CAE)/100 g and 1.71 mg naringin equivalent (NE)/100 g, respectively, and DPPH radical scavenging activities was 90%. Among the tested organic solvents, hexane gave the highest extraction yield. Thus hexane was selected as the most suitable solvent for the extraction of thyme. Response surface method was used to obtain optimized extraction conditions for thyme: reaction temperature of 35.9 ℃, raw material to hexane ratio of 1.63:25 (w/v), and reaction time of 192 min. These predicted extraction conditions was validated by a total flavonoid extraction experiment showing a value equivalent to 96.3% of the predicted total flavonoid content. It is expected that the optimized solvent extraction conditions could be used for the production of flavonoid using thyme.
Keywords
Extraction conditions; Flavonoid; Optimization Response surface method; Thyme (Thymus vulgaris Libiatae);
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