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http://dx.doi.org/10.3839/jabc.2014.003

Mechanism for Antioxidant Activity of Nardostachys chinensis root Extract  

Heo, Jee-In (Department of Biochemistry, Hallym University Medical School)
Kim, Jeong-Hyeon (Department of Biochemistry, Hallym University Medical School)
Lee, Jeong-Min (Department of Biochemistry, Hallym University Medical School)
Kim, Sung Chan (Department of Biochemistry, Hallym University Medical School)
Park, Jae-Bong (Department of Biochemistry, Hallym University Medical School)
Kim, Jaebong (Department of Biochemistry, Hallym University Medical School)
Lee, Jae-Yong (Department of Biochemistry, Hallym University Medical School)
Publication Information
Journal of Applied Biological Chemistry / v.57, no.1, 2014 , pp. 17-22 More about this Journal
Abstract
Nardostachys chinensis (N. chinensis) has been used in traditional medicine as a sedative and analgesic. It has been reported that N. chinensis extract has an antioxidant activity. However, the mechanism has not been elucidated. In this study, we showed that FOXO3a was activated by N. chinensis extract. FOXO3a is a transcriptional factor that involved in cell cycle arrest, DNA repair, apoptosis, and detoxification of reactive oxygen spices (ROS). Protein level of FOXO3a was increased by N. chinensis extract whereas phospho-FOXO3a (Thr 32) was not changed. Promoter activities of target genes of FOXO3a such as MnSOD, p27, and GADD45 were increased by N. chinensis extract. Among target genes, protein level of MnSOD was increased by N. chinensis extract, and this leads to removal of ROS level in human embryonic fibroblast (HEF) cells. These results suggested that N. chinensis extract has an antioxidant activity by upregulation of MnSOD through FOXO3a activation.
Keywords
antioxidant; FOXO3a; MnSOD; Nardostachys chinensis; reactive oxygen species;
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