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http://dx.doi.org/10.3839/jabc.2011.014

Nitrate Uptake by Soil Microorganism, Bacillus sp. GS2  

Wang, Hee-Sung (Department of Environmental & Biological Chemistry, Chungbuk National University)
Yoon, Young-Bae (Department of Environmental & Biological Chemistry, Chungbuk National University)
Kim, Young-Kee (Department of Environmental & Biological Chemistry, Chungbuk National University)
Publication Information
Journal of Applied Biological Chemistry / v.54, no.2, 2011 , pp. 79-83 More about this Journal
Abstract
Over-application of nitrogen fertilizer keeps increasing the salinity in the soils of greenhouse in domestic agriculture. In order to remove the excess amounts of soil nitrate, soil microorganisms which have high capacity of nitrate uptake were isolated from the upland soils and their nitrate uptake activities were measured. Strain GS2 was able to remove 50 mM nitrate within 12 h. After sequence comparison analysis of 16S rRNA gene, the strain was identified and named as Bacillus sp. GS2. When the growth and nitrate uptake activities were measured, maximal values were obtained at $30-40^{\circ}C$ and $37^{\circ}C$, respectively; however, both were optimal at pH 6-8. In the media containing 50 mM nitrate, Bacillus sp. GS2 removed 43 mM nitrate which is corresponding to 86% removal. Similar amounts of nitrate removal were observed at the nitrate concentrations up to 300 mM, showing a saturation in nitrate uptake at concentrations above 50 mM. These results imply that Bacillus sp. GS2 can be a good candidate for the microbial remediation of accumulated environmental nitrate because of its excellent growth and nitrate uptake activity.
Keywords
nitrate removal; rRNA gene; salt effect; soil salinity;
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