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http://dx.doi.org/10.3839/jabc.2009.010

Expression Patterns of Enzymes in Different Tissues of Oil Seed Rape (Brassica napus L.) Seedling  

Song, Yong-Su (Environment-Friendly Agriculture Research Center (EFARC), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University)
Seo, Dong-Jun (Environment-Friendly Agriculture Research Center (EFARC), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University)
Lee, Bok-Rye (Department of Animal Science, Institute of Agricultural Science and Technology, Chonnam National University)
Jung, Woo-Jin (Environment-Friendly Agriculture Research Center (EFARC), Division of Applied Bioscience and Biotechnology, Institute of Agricultural Science and Technology, Chonnam National University)
Publication Information
Journal of Applied Biological Chemistry / v.52, no.2, 2009 , pp. 51-57 More about this Journal
Abstract
To investigate expression patterns of chitinase, ${\beta}$-1,3-glucanase and peroxidase involved in biological control of phytopathogens, three oil seed rapes (Capitol, Pollen and Saturnin) were used. Activities of the enzymes in old leaves were $9.7{\sim}11.8$ unit/mg protein in chitinase, $11.1{\sim}17.3$ unit/mg protein in ${\beta}$-1,3-glucanase and $0.6{\sim}1.7$ unit/mg protein in peroxidase. Activities of the enzymes in roots were $39.2{\sim}49.0$ unit/mg protein in chitinase, $49.9{\sim}62.0$ unit/mg protein in ${\beta}$-1,3-glucanase and $2.4{\sim}3.8$ unit/mg protein in peroxidase. Chitinase and ${\beta}$-1,3-glucanase activity were the highest level in Saturnin leaves and in Capitol roots while activities of those were the lowest level in Capitol leaves. Also, chitinase and ${\beta}$-1,3-glucanase and peroxidase activity were the lowest level in Saturnin roots. Active bands of chitinase isoform in leaves (73, 51, 40, 34, and 29 kDa) and in roots (100, 57 34, and 29 kDa) tissues showed in the SDS-PAGE gel. Active bands of ${\beta}$-1,3-glucanase isoform in leaves and roots (75 and 55 kDa) tissues showed on the SDS-PAGE gel. Active staining of peroxidase showed the strongest level in leaves and roots of Pollen. Active bands of peroxidase isoform in leaves (122, 114, and 93 kDa) and in roots (135, 122, 114, and 93 kDa) tissues showed on the Native-PAGE gel. These results indicated that establishment of expression pattern of enzymes in rape tissues could play as an important role with respect to resistance of plant pathogens in rape.
Keywords
Brassica napus L.; chitinase; ${\beta}$-1,3-glucanase;
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Times Cited By KSCI : 1  (Citation Analysis)
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