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http://dx.doi.org/10.4162/nrp.2014.8.5.509

Vitis amurensis Ruprecht root inhibited ${\alpha}$-melanocyte stimulating hormone-induced melanogenesis in B16F10 cells  

Jin, Kyong-Suk (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Oh, You Na (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Hyun, Sook Kyung (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Kwon, Hyun Ju (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Kim, Byung Woo (Blue-Bio Industry Regional Innovation Center, Dong-Eui University)
Publication Information
Nutrition Research and Practice / v.8, no.5, 2014 , pp. 509-515 More about this Journal
Abstract
BACKGROUND/OBJECTIVES: The root of Vitis amurensis Ruprecht, a sort of wild-growing grape, has been used in oriental medicine for treatment of skin ailments; however, its dermatological activity is not sufficiently understood. The aim of this study was to investigate tyrosinase inhibitory and anti-melanogenic activities of V. amurensis Ruprecht root methanol extract (VARM) in B16F10 mouse melanoma cells and to attempt to isolate and identify the active compound issued from VARM. MATERIALS/METHODS: Anti-melanogenic activity of VARM was analyzed in ${\alpha}$-melanocyte stimulating hormone (MSH)-stimulated B16F10 cells through evaluation of antioxidative activity as well as inhibited tyrosinase activity and melanin contents compared with those of kojic acid and arbutin. After anti-melanogenic analysis of VARM, serial fractionation, nuclear magnetic resonance (NMR), and thin layer chromatorgraphy (TLC) were applied for identification of active compounds contained in VARM. RESULTS: VARM significantly inhibited oxidative stress and tyrosinase activity and attenuated ${\alpha}$-MSH-induced melanin production in B16F10 cells. For isolation of active compounds, VARM was fractionated using a series of organic solvents, including dichloromethane ($CH_2Cl_2$), ethyl acetate (EtOAc), and n-butanol (n-BuOH). Among fractions showing anti-melanogenic activity, the CH2Cl2 fraction induced the most potent attenuation of melanogenesis without cytotoxicity and the major compound in the $CH_2Cl_2$ fraction was identified as betulinic acid. Betulinic acid isolated from the $CH_2Cl_2$ fraction of VARM significantly attenuated ${\alpha}$-MSH-induced melanogenesis in a dose dependent manner, which was stronger than that of arbutin used as a positive control. CONCLUSIONS: These results indicate that VARM inhibits oxidative stress, tyrosinase activity, and ${\alpha}$-MSH-induced melanogenesis in B16F10 cells, due primarily to the active compound, betulinic acid, in the $CH_2Cl_2$ fraction.
Keywords
Vitis amurensis Ruprecht root; anti-oxidation; tyrosinase; melanogenesis; betulinic acid;
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