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http://dx.doi.org/10.4162/nrp.2013.7.2.89

Inorganic sulfur reduces cell proliferation by inhibiting of $ErbB_2$ and $ErbB_3$ protein and mRNA expression in MDA-MB-231 human breast cancer cells  

Ha, Ae Wha (Department of Food Science and Nutrition, Dankook University)
Hong, Kyung Hee (Department of Food and Nutrition, Beawha Women's University)
Kim, Hee Sun (Department of Food and Nutrition, Beawha Women's University)
Kim, Woo Kyoung (Department of Food Science and Nutrition, Dankook University)
Publication Information
Nutrition Research and Practice / v.7, no.2, 2013 , pp. 89-95 More about this Journal
Abstract
Dietary inorganic sulfur is the minor component in our diet, but some studies suggested that inorganic sulfur is maybe effective to treat cancer related illness. Therefore, this study aims to examine the effects of inorganic sulfur on cell proliferation and gene expression in MDA-MB-231 human breast cancer cells. MDA-MB-231 cells were cultured the absence or presence of various concentrations (12.5, 25, or 50 ${\mu}mol/L$) of inorganic sulfur. Inorganic sulfur significantly decreased proliferation after 72 h of incubation (P < 0.05). The protein expression of $ErbB_2$ and its active form, $pErbB_2$, were significantly reduced at inorganic sulfur concentrations of 50 ${\mu}mol/L$ and greater than 25 ${\mu}mol/L$, respectively (P < 0.05). The mRNA expression of $ErbB_2$ was significantly reduced at an inorganic sulfur concentration of 50 ${\mu}mol/L$ (P < 0.05). The protein expression of $ErbB_3$ and its active form, $pErbB_3$, and the mRNA expression of $pErbB_3$ were significantly reduced at inorganic sulfur concentrations greater than 25 ${\mu}mol/L$ (P < 0.05). The protein and mRNA expression of Akt were significantly reduced at an inorganic sulfur concentration of 50 ${\mu}mol/L$ (P < 0.05), but pAkt was not affected by inorganic sulfur treatment. The protein and mRNA expression of Bax were significantly increased with the addition of inorganic sulfur concentration of 50 ${\mu}mol/L$ (P < 0.05). In conclusion, cell proliferation was suppressed by inorganic sulfur treatment through the ErbB-Akt pathway in MDA-MB-231 cells.
Keywords
Inorganic sulfur; epidermal growth factor receptor; apoptosis; cell proliferation; MDA-MB-231 cell;
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1 Cardone MH, Roy N, Stennicke HR, Salvesen GS, Franke TF, Stanbridge E, Frisch S, Reed JC. Regulation of cell death protease caspase-9 by phosphorylation. Science 1998;282:1318-21.   DOI
2 del Peso L, Gonzalez-Garcia M, Page C, Herrera R, Nunez G. Interleukin-3-induced phosphorylation of BAD through the protein kinase Akt. Science 1997;278:687-9.   DOI   ScienceOn
3 Brunet A, Bonni A, Zigmond MJ, Lin MZ, Juo P, Hu LS, Anderson MJ, Arden KC, Blenis J, Greenberg ME. Akt promotes cell survival by phosphorylating and inhibiting a forkhead transcription factor. Cell 1999;96:857-68.   DOI   ScienceOn
4 Takeuchi K, Ito F. Suppression of adriamycin-induced apoptosis by sustained activation of the phosphatidylinositol-3'-OH kinase- Akt pathway. J Biol Chem 2004;279:892-900.   DOI
5 Binder C, Marx D, Binder L, Schauer A, Hiddemann W. Expression of Bax in relation to Bcl-2 and other predictive parameters in breast cancer. Ann Oncol 1996;7:129-33.   DOI   ScienceOn
6 Keane MM, Ettenberg SA, Lowrey GA, Russell EK, Lipkowitz S. Fas expression and function in normal and malignant breast cell lines. Cancer Res 1996;56:4791-8.
7 Wolter KG, Hsu YT, Smith CL, Nechushtan A, Xi XG, Youle RJ. Movement of Bax from the cytosol to mitochondria during apoptosis. J Cell Biol 1997;139:1281-92.   DOI   ScienceOn
8 Gardai SJ, Hildeman DA, Frankel SK, Whitlock BB, Frasch SC, Borregaard N, Marrack P, Bratton DL, Henson PM. Phosphorylation of Bax Ser184 by Akt regulates its activity and apoptosis in neutrophils. J Biol Chem 2004;279:21085-95.   DOI   ScienceOn
9 Cho HJ, Kim WK, Kim EJ, Jung KC, Park S, Lee HS, Tyner AL, Park JH. Conjugated linoleic acid inhibits cell proliferation and ErbB3 signaling in HT-29 human colon cell line. Am J Physiol Gastrointest Liver Physiol 2003;284:G996-1005.   DOI
10 Kong CS, Bak SS, Rhee SH, Rho CW, Kim NK. Fermentation properties of young radish Kimchi prepared using young radish cultivated in the soil containing sulfur and it's inhibitory effect on the growth of AGS human gastric adenocarcinoma cells. J Korean Soc Food Sci Nutr 2006;35:158-63.   DOI   ScienceOn
11 Bak SS, Kong CS, Rhee SH, Rho CW, Kim NK, Choi KL, Park KY. Effect of sulfur enriched young radish Kimchi on the induction of apoptosis in HT-29 human colon cancer cells. J Food Sci Nutr 2006;11:184-90.   DOI   ScienceOn
12 Choi GH, Kim CH. Growth inhibition of extract from sulfur fed duck carcass against various cancer cell lines. J Korean Soc Food Sci Anim Resour 2002;22:348-51.
13 Gamet-Payrastre L, Li P, Lumeau S, Cassar G, Dupont MA, Chevolleau S, Gasc N, Tulliez J, Terce F. Sulforaphane, a naturally occurring isothiocyanate, induces cell cycle arrest and apoptosis in HT29 human colon cancer cells. Cancer Res 2000;60:1426-33.
14 Singh AV, Xiao D, Lew KL, Dhir R, Singh SV. Sulforaphane induces caspase-mediated apoptosis in cultured PC-3 human prostate cancer cells and retards growth of PC-3 xenografts in vivo. Carcinogenesis 2004;25:83-90.
15 Takeuchi K, Ito F. EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFRtargeting tyrosine kinase inhibitors. FEBS J 2010;277:316-26.   DOI   ScienceOn
16 Han SW, Choi YY, Woo HD, Sohn DM, Bae SH, Gang GH, Kim SY, Baek MJ, Lim CW, Lee MS, Kim CH, Lee MH, Rho JH, Cho HD, Oh MH, Kim EH, Cho MS. Expression of HER-2/ neu and paxillin in ductal carcinoma in situ, invasive ductal carcinoma with ductal carcinoma in situ and mucinous carcinoma. J Breast Cancer 2008;11:109-15.   DOI   ScienceOn
17 Burgess AW, Cho HS, Eigenbrot C, Ferguson KM, Garrett TP, Leahy DJ, Lemmon MA, Sliwkowski MX, Ward CW, Yokoyama S. An open-and-shut case? Recent insights into the activation of EGF/ErbB receptors. Mol Cell 2003;12:541-52.   DOI   ScienceOn
18 Citri A, Yarden Y. EGF-ERBB signalling: towards the systems level. Nat Rev Mol Cell Biol 2006;7:505-16.   DOI   ScienceOn
19 Guy CT, Webster MA, Schaller M, Parsons TJ, Cardiff RD, Muller WJ. Expression of the neu protooncogene in the mammary epithelium of transgenic mice induces metastatic disease. Proc Natl Acad Sci U S A 1992;89:10578-82.   DOI   ScienceOn
20 Pao W, Miller V, Zakowski M, Doherty J, Politi K, Sarkaria I, Singh B, Heelan R, Rusch V, Fulton L, Mardis E, Kupfer D, Wilson R, Kris M, Varmus H. EGF receptor gene mutations are common in lung cancers from "never smokers" and are associated with sensitivity of tumors to gefitinib and erlotinib. Proc Natl Acad Sci USA 2004;101:13306-11.   DOI   ScienceOn
21 Janmaat ML, Kruyt FA, Rodriguez JA, Giaccone G. Response to epidermal growth factor receptor inhibitors in non-small cell lung cancer cells: limited antiproliferative effects and absence of apoptosis associated with persistent activity of extracellular signal-regulated kinase or Akt kinase pathways. Clin Cancer Res 2003;9:2316-26.
22 Ingenbleek Y. The nutritional relationship linking sulfur to nitrogen in living organisms. J Nutr 2006;136:1641S-1651S.   DOI
23 Joo CN, Choi SD. Sulfur metabolism in animal body -absorption of sulfur from intestine and its distribution-. Korean Biochem J 1992;25:397-402.
24 Magee EA, Richardson CJ, Hughes R, Cummings JH. Contribution of dietary protein to sulfide production in the large intestine: an in vitro and a controlled feeding study in humans. Am J Clin Nutr 2000;72:1488-94.   DOI
25 Lin AN, Reimer RJ, Carter DM. Sulfur revisited. J Am Acad Dermatol 1988;18:553-8.   DOI   ScienceOn
26 National Institute of Food and Drug Safety Evaluation [Internet]. Sulfur. Cheongwon: National Institute of Food and Drug Safety Evaluation; 2010 [cited 2010 August 10]. Available from: http://www. nifds.go.kr/toxinfo/Index.
27 Pappa A, Franco R, Schoneveld O, Galanis A, Sandaltzopoulos R, Panayiotidis MI. Sulfur-containing compounds in protecting against oxidant-mediated lung diseases. Curr Med Chem 2007;14: 2590-6.   DOI   ScienceOn
28 Pratsel HG, Eigner UM, Weinert D, Limbach B. The analgesic efficacy of sulfur mud baths in treating rheumatic diseases of the soft tissues. A study using the double-blind control method. Vopr Kurortol Fizioter Lech Fiz Kult 1992;(3):37-41.
29 Jung E. Sulfur, oil and tar-oil baths in geriatrics. Z Krankenpfl 1971;64:230-2.
30 Brown RG, Button GM, Smith JT. Changes in collagen metabolism caused by feeding diets low in inorganic sulfur. J Nutr 1965;87:228-32.   DOI
31 Button GM, Brown RG, Michels FG, Smith JT. Utilization of calcium and sodium sulfate by the rat. J Nutr 1965;87:211-6.   DOI
32 Kerr BJ, Weber TE, Ziemer CJ, Spence C, Cotta MA, Whitehead TR. Effect of dietary inorganic sulfur level on growth performance, fecal composition, and measures of inflammation and sulfate-reducing bacteria in the intestine of growing pigs. J Anim Sci 2011;89:426-37.   DOI   ScienceOn
33 Anast C, Kennedy R, Volk G, Adamson L. In vitro studies of sulfate transport by the small intestine of the rat, rabbit, and hamster. J Lab Clin Med 1965;65:903-11.
34 Sasse CE, Baker DH. Sulfur utilization by the chick with emphasis on the effect of inorganic sulfate on the cystine-methionine interrelationship. J Nutr 1974;104:244-51.   DOI
35 Parcell S. Sulfur in human nutrition and applications in medicine. Altern Med Rev 2002;7:22-44.
36 Kim EJ, Kang IJ, Cho HJ, Kim WK, Ha YL, Park JH. Conjugated linoleic acid downregulates insulin-like growth factor-I receptor levels in HT-29 human colon cancer cells. J Nutr 2003;133:2675-81.   DOI
37 Lee HS, Seo EY, Kim WK. Resveratrol induces apoptosis in SW480 human colon cancer cell lines. Food Sci Biotechnol 2004;13:80-4.