Browse > Article
http://dx.doi.org/10.4162/nrp.2012.6.6.505

Improvement of andropause symptoms by dandelion and rooibos extract complex CRS-10 in aging male  

Noh, Yoo-Hun (Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University)
Kim, Do-Hee (Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University)
Kim, Joon Yub (Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University)
Park, Jiae (Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University)
Kim, Ok Hyeon (Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University)
Han, Daeseok (Korea Food Research Institute)
Kim, Won-Yong (Department of Microbiology, College of Medicine, Chung-Ang University)
Kim, Sung-Su (Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University)
Lee, Moo-Yeol (Department of Physiology, College of Medicine, Chung-Ang University)
Heo, Seok-Hyun (Korea Health Supplements Institute)
Kim, Misook (Department of Food Science and Nutrition, College of Natural Science, Dankook University)
Lee, Won Bok (Department of Anatomy and Cell Biology, College of Medicine, Chung-Ang University)
Jeong, Yoonhwa (Department of Food Science and Nutrition, College of Natural Science, Dankook University)
Myung, Soon Chul (Department of Urology, College of Medicine, Chung-Ang University)
Publication Information
Nutrition Research and Practice / v.6, no.6, 2012 , pp. 505-512 More about this Journal
Abstract
Many aging male suffer various andropause symptoms including loss of physical and mental activities. This study evaluated the putative alleviative effects of CRS-10 dandelion and rooibos extract complex (CRS-10) on the symptoms of andropause. The survival rate of TM3 Leydig cells (TM3 cells) treated with CRS-10 was measured based on typical physiological stress. After daily intake of CRS-10 for 4 weeks, the level of testosterone, physical activity and both the number and activity of sperm in older rats (18 weeks) were measured. Furthermore, thirty males were surveyed with AMS (Aging Males' Symptoms) questionnaire after intake of 400 mg of CRS-10. Overall, CRS-10 protected TM3 cells from serum restriction and oxidative stress via activation of ERK and Akt pathways. The level of testosterone and activation of spermatogenesis in rats were significantly enhanced. In addition, physical locomotion was markedly improved. Daily intake of 400 mg of CRS-10 improved the quality of life among agingmale respondents, according to a clinical survey using the AMS. The results indicate the potential of CRS-10 as a safe and efficacious natural substance for reducing or alleviating andropause symptoms.
Keywords
Leydig cell; testosterone; CRS-10; spermatogenesis; andropause;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Ibebunjo C, Eash JK, Li C, Ma Q, Glass DJ. Voluntary running, skeletal muscle gene expression, and signaling inversely regulated by orchidectomy and testosterone replacement. Am J Physiol Endocrinol Metab 2011;300:E327-40.   DOI
2 Nigro N, Christ-Crain M. Testosterone treatment in the aging male: myth or reality? Swiss Med Wkly 2012;142:w13539.
3 Barqawi A, Crawford ED. Testosterone replacement therapy and the risk of prostate cancer. Is there a link? Int J Impot Res 2006; 18:323-8.   DOI   ScienceOn
4 Zhang Y, Ge R, Hardy MP. Androgen-forming stem Leydig cells: identification, function and therapeutic potential. Dis Markers 2008;24:277-86.   DOI   ScienceOn
5 Chen L, Zhao Y, Zhang Y. Progress on the research of stem Leydig cell line in the testis of rodents. Wei Sheng Yan Jiu 2012;41:158-62.
6 He W, Han H, Wang W, Gao B. Anti-influenza virus effect of aqueous extracts from dandelion. Virol J 2011;8:538.   DOI
7 Huang Y, Wu T, Zeng L, Li S. Chinese medicinal herbs for sore throat. Cochrane Database Syst Rev 2012;3:CD004877.
8 Ovadje P, Chatterjee S, Griffin C, Tran C, Hamm C, Pandey S. Selective induction of apoptosis through activation of caspase-8 in human leukemia cells (Jurkat) by dandelion root extract. J Ethnopharmacol 2011;133:86-91.   DOI   ScienceOn
9 Utter AC, Quindry JC, Emerenziani GP, Valiente JS. Effects of rooibos tea, bottled water, and a carbohydrate beverage on blood and urinary measures of hydration after acute dehydration. Res Sports Med 2010;18:85-96.   DOI   ScienceOn
10 Darvesh AS, Carroll RT, Bishayee A, Geldenhuys WJ, Van der Schyf CJ. Oxidative stress and Alzheimer's disease: dietary polyphenols as potential therapeutic agents. Expert Rev Neurother 2010;10:729-45.   DOI   ScienceOn
11 Bramati L, Aquilano F, Pietta P. Unfermented rooibos tea: quantitative characterization of flavonoids by HPLC-UV and determination of the total antioxidant activity. J Agric Food Chem 2003;51:7472-4.   DOI   ScienceOn
12 Awoniyi DO, Aboua YG, Marnewick J, Brooks N. The effects of rooibos (Aspalathus linearis), green tea (Camellia sinensis) and commercial rooibos and green tea supplements on epididymal sperm in oxidative stress-induced rats. Phytother Res 2012;26: 1231-9.   DOI   ScienceOn
13 Marnewick JL, Rautenbach F, Venter I, Neethling H, Blackhurst DM, Wolmarans P, Macharia M. Effects of rooibos (Aspalathus linearis) on oxidative stress and biochemical parameters in adults at risk for cardiovascular disease. J Ethnopharmacol 2011;133: 46-52.   DOI   ScienceOn
14 Baba H, Ohtsuka Y, Haruna H, Lee T, Nagata S, Maeda M, Yamashiro Y, Shimizu T. Studies of anti-inflammatory effects of rooibos tea in rats. Pediatr Int 2009;51:700-4.   DOI   ScienceOn
15 Tan RS, Pu SJ. Impact of obesity on hypogonadism in the andropause. Int J Androl 2002;25:195-201.   DOI   ScienceOn
16 Vermeulen A, Kaufman JM. Ageing of the hypothalamo-pituitarytesticular axis in men. Horm Res 1995;43:25-8.   DOI   ScienceOn
17 Vermeulen A. Environment, human reproduction, menopause, and andropause. Environ Health Perspect 1993;101 Suppl 2:91-100.   DOI
18 Bornman MS, Reif S. Serum testosterone levels in South African men and the onset of androgen decline in ageing males. S Afr J Surg 2007;45:62, 64.
19 Moon C, Kim JS, Jang H, Lee HJ, Kim SH, Kang SS, Bae CS, Kim JC, Kim S, Lee Y, Shin T. Activation of Akt/protein kinase B and extracellular signal-regulated kinase in rats with acute experimental testicular torsion. J Vet Med Sci 2008;70:337-41.   DOI   ScienceOn
20 Tenover JL. Male hormone replacement therapy including "andropause". Endocrinol Metab Clin North Am 1998;27:969-87, x.   DOI   ScienceOn
21 Hu C, Kitts DD. Dandelion (Taraxacum officinale) flower extract suppresses both reactive oxygen species and nitric oxide and prevents lipid oxidation in vitro. Phytomedicine 2005;12:588-97.   DOI   ScienceOn
22 Kim JY, Han EH, Kim HG, Oh KN, Kim SK, Lee KY, Jeong HG. Bisphenol A-induced aromatase activation is mediated by cyclooxygenase-2 up-regulation in rat testicular Leydig cells. Toxicol Lett 2010;193:200-8.   DOI   ScienceOn
23 Yamashita S, Tai P, Charron J, Ko C, Ascoli M. The Leydig cell MEK/ERK pathway is critical for maintaining a functional population of adult Leydig cells and for fertility. Mol Endocrinol 2011;25:1211-22.   DOI   ScienceOn
24 Walsh PC, Swerdloff RS. Biphasic effect of testosterone on spermatogenesis in the rat. Invest Urol 1973;11:190-3.
25 Chen H, Liu J, Luo L, Zirkin BR. Dibutyryl cyclic adenosine monophosphate restores the ability of aged Leydig cells to produce testosterone at the high levels characteristic of young cells. Endocrinology 2004;145:4441-6.   DOI   ScienceOn
26 Horstman AM, Dillon EL, Urban RJ, Sheffield-Moore M. The role of androgens and estrogens on healthy aging and longevity. J Gerontol A Biol Sci Med Sci 2012;67:1140-52.   DOI   ScienceOn
27 Bormann CL, Smith GD, Padmanabhan V, Lee TM. Prenatal testosterone and dihydrotestosterone exposure disrupts ovine testicular development. Reproduction 2011;142:167-73.   DOI
28 Sofikitis N, Giotitsas N, Tsounapi P, Baltogiannis D, Giannakis D, Pardalidis N. Hormonal regulation of spermatogenesis and spermiogenesis. J Steroid Biochem Mol Biol 2008;109:323-30.   DOI   ScienceOn
29 Schwartz E, Morelli V, Holtorf K. Hormone replacement therapy in the geriatric patient: current state of the evidence and questions for the future--estrogen, progesterone, testosterone, and thyroid hormone augmentation in geriatric clinical practice: part 2. Clin Geriatr Med 2011;27:561-75.   DOI   ScienceOn
30 Schwartz E, Holtorf K. Hormone replacement therapy in the geriatric patient: current state of the evidence and questions for the future. Estrogen, progesterone, testosterone, and thyroid hormone augmentation in geriatric clinical practice: part 1. Clin Geriatr Med 2011;27:541-59.   DOI   ScienceOn
31 Heinemann LA. Aging Males' Symptoms scale: a standardized instrument for the practice. J Endocrinol Invest 2005;28:34-8.   DOI
32 Miwa Y, Kaneda T, Yokoyama O. Correlation between the Aging Males' Symptoms scale and sex steroids, gonadotropins, dehydroepiandrosterone sulfate, and growth hormone levels in ambulatory men. J Sex Med 2006;3:723-6.   DOI   ScienceOn
33 Kawa G, Taniguchi H, Kinoshita H, Matsuda T, Urakami M, Sawaragi I. Aging Male Symptoms and serum testosterone levels in healthy Japanese middle-aged men. Nihon Hinyokika Gakkai Zasshi 2008;99:645-51.
34 Travison TG, Basaria S, Storer TW, Jette AM, Miciek R, Farwell WR, Choong K, Lakshman K, Mazer NA, Coviello AD, Knapp PE, Ulloor J, Zhang A, Brooks B, Nguyen AH, Eder R, LeBrasseur N, Elmi A, Appleman E, Hede-Brierley L, Bhasin G, Bhatia A, Lazzari A, Davis S, Ni P, Collins L, Bhasin S. Clinical meaningfulness of the changes in muscle performance and physical function associated with testosterone administration in older men with mobility limitation. J Gerontol A Biol Sci Med Sci 2011; 66:1090-9.
35 Wang C, Hikim AS, Ferrini M, Bonavera JJ, Vernet D, Leung A, Lue YH, Gonzalez-Cadavid NF, Swerdloff RS. Male reproductive ageing: using the brown Norway rat as a model for man. Novartis Found Symp 2002;242:82-95; discussion 95-7.
36 Luo L, Chen H, Zirkin BR. Are Leydig cell steroidogenic enzymes differentially regulated with aging? J Androl 1996;17:509-15.
37 Wu CY, Yu TJ, Chen MJ. Age related testosterone level changes and male andropause syndrome. Chang Gung Med J 2000;23: 348-53.
38 Sattler F, Bhasin S, He J, Chou CP, Castaneda-Sceppa C, Yarasheski K, Binder E, Schroeder ET, Kawakubo M, Zhang A, Roubenoff R, Azen S. Testosterone threshold levels and lean tissue mass targets needed to enhance skeletal muscle strength and function: the HORMA trial. J Gerontol A Biol Sci Med Sci 2011;66:122-9.
39 Chen RY, Ng KK. Self-referred older Asian males in a men's health clinic: the inter-relationships between androgens, metabolic parameters and quality of life measures. Aging Male 2010;13: 233-41.   DOI   ScienceOn