Browse > Article
http://dx.doi.org/10.4162/nrp.2011.5.5.381

Comparative effect of genistein and daidzein on the expression of MCP-l, eNOS, and cell adhesion molecules in TNF-${\alpha}$-stimulated HUVECs  

Cho, Hye-Yeon (Paik Institute for Clinical Research, Inje University)
Park, Chung-Mu (Department of Smart Foods and Drugs, Inje University)
Kim, Mi-Jeong (Department of Food and Nutrition, Silla University)
Chinzorig, Radnaabazar (Department of Smart Foods and Drugs, Inje University)
Cho, Chung-Won (School of Biomedical and Biotechnology, Inje University)
Song, Young-Sun (Department of Smart Foods and Drugs, Inje University)
Publication Information
Nutrition Research and Practice / v.5, no.5, 2011 , pp. 381-388 More about this Journal
Abstract
We compared the effects of genistein and daidzein on the expression of chemokines, cell adhesion molecules (CAMs), and endothelial nitric oxide synthase (eNOS) in tumor necrosis factor (TNF)-${\alpha}$-stimulated human umbilical vascular endothelial cells (HUVECs). TNF-${\alpha}$ exposure significantly increased expression of monocyte chemoattractant protein (MCP)-l, vascular adhesion molecule (VCAM)-1, and intercellular adhesion molecule-1. Genistein significantly decreased MCP-l and VCAM-l production in a dose-dependent manner, whereas CAM expression was not significantly lowered by genistein treatment. However, daidzein slightly decreased MCP-l production. The effects of genistein and daidzein on MCP-l secretion coincided with mRNA expression. Pre-treatment with either genistein or daidzein elevated eNOS expression and nitric oxide production disturbed by TNF-${\alpha}$ exposure. A low concentration of isoflavones significantly inhibited nuclear factor (NF)${\kappa}$B activation, whereas a high dose slightly ameliorated these inhibitive effects. These results suggest that genistein had a stronger effect on MCP-l and eNOS expression than that of daidzein. Additionally, NF${\kappa}$B transactivation might be partially related to the down-regulation of these mRNAs in TNF-${\alpha}$-stimulated HUVECs.
Keywords
Genistein; MCP-l; cell adhesion molecules; eNOS; NF${\kappa}$B;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 3  (Related Records In Web of Science)
연도 인용수 순위
1 Chen H, Chen Y, Tian W, Lei S, Peng R. Effects of estradiol and isoflavoid on the expression of adhesion molecules on neutrophils. Hua Xi Yi Ke Da Xue Xue Bao 2001;32:27-31.
2 Lund CO, Mortensen A, Nilas L, Breinholt VM, Larsen JJ, Ottesen B. Estrogen and phytoestrogens: Effect on eNOS expression and in vitro vasodilation in cerebral arteries in ovariectomized Watanabe heritable hyperlipidemic rabbits. Eur J Obstet Gynecol Reprod Biol 2007;130:84-92.   DOI   ScienceOn
3 Adamson P, Tighe M, Pearson JD. Protein tyrosine kinase inhibitors act downstream of IL-1 alpha and LPS stimulated MAP-kinase phosphorylation to inhibit expression of E-selectin on human umbilical vein endothelial cells. Cell Adhes Commun 1996;3:511-25.   DOI
4 Majewska E, Paleolog E, Baj Z, Kralisz U, Feldmann M, Tchorzewski H. Role of tyrosine kinase enzymes in TNF-alpha and IL-1 induced expression of ICAM-1 and VCAM-1 on human umbilical vein endothelial cells. Scand J Immunol 1997;45:385-92.   DOI   ScienceOn
5 Lee YS, Jang SY, Kim KO. Effects of soy isoflavone intake on nitrite content and antioxidant enzyme activities in male rats fed high-fat diet. Korean J Nutr 2005;38:89-95.
6 Liu D, Homan LL, Dillon JS. Genistein acutely stimulates nitric oxide synthesis in vascular endothelial cells by a cyclic adenosine 5'-monophosphate-dependent mechanism. Endocrinology 2004;145:5532-9.   DOI   ScienceOn
7 Yen GC, Lai HH. Inhibitory effects of isoflavones on nitric oxide- or peroxynitrite-mediated DNA damage in RAW 264.7 cells and phiX174 DNA. Food Chem Toxicol 2002;40:1433-40.   DOI   ScienceOn
8 Sheu F, Lai HH, Yen GC. Suppression effect of soy isoflavones on nitric oxide production in RAW 264.7 macrophages. J Agric Food Chem 2001;49:1767-72.   DOI   ScienceOn
9 Mizutani K, Ikeda K, Nishikata T, Yamori Y. Phytoestrogens attenuate oxidative DNA damage in vascular smooth muscle cells from stroke-prone spontaneously hypertensive rats. J Hypertens 2000;18:1833-40.   DOI   ScienceOn
10 Ruiz-Larrea MB, Mohan AR, Paganga G, Miller NJ, Bolwell GP, Rice-Evans CA. Antioxidant activity of phytoestrogenic isoflavones. Free Radic Res 1997;26:63-70.   DOI   ScienceOn
11 Wei H, Wei L, Frenkel K, Bowen R, Barnes S. Inhibition of tumor promoter-induced hydrogen peroxide formation in vitro and in vivo by genistein. Nutr Cancer 1993;20:1-12.   DOI   ScienceOn
12 Kapiotis S, Hermann M, Held I, Seelos C, Ehringer H, Gmeiner BM. Genistein, the dietary-derived angiogenesis inhibitor, prevents LDL oxidation and protects endothelial cells from damage by atherogenic LDL. Arterioscler Thromb Vasc Biol 1997;17:2868-74.   DOI   ScienceOn
13 Sadowska-Krowicka H, Mannick EE, Oliver PD, Sandoval M, Zhang XJ, Eloby-Childess S, Clark DA, Miller MJ. Genistein and gut inflammation: role of nitric oxide. Proc Soc Exp Biol Med 1998;217:351-7.
14 Anthony MS, Clarkson TB, Hughes CL Jr, Morgan TM, Burke GL. Soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkeys. J Nutr 1996;126:43-50.
15 Lee YM, Jung MH, Lee YS, Song J. Effect of genistein and soy protein on lipids metabolism in ovariectomized rats. Korean J Nutr 2005;38:267-78.
16 Kirk EA, Sutherland P, Wang SA, Chait A, LeBoeuf RC. Dietary isoflavones reduce plasma cholesterol and atherosclerosis in C57BL/6 mice but not LDL receptor-deficient mice. J Nutr 1998;128:954-9.
17 Vera R, Sanchez M, Galisteo M, Villar IC, Jimenez R, Zarzuelo A, Perez-Vizcaino F, Duarte J. Chronic administration of genistein improves endothelial dysfunction in spontaneously hypertensive rats: involvement of eNOS, caveolin and calmodulin expression and NADPH oxidase activity. Clin Sci (Lond) 2007;112:183-91.   DOI   ScienceOn
18 Mahn K, Borras C, Knock GA, Taylor P, Khan IY, Sugden D, Poston L, Ward JP, Sharpe RM, Vina J, Aaronson PI, Mann GE. Dietary soy isoflavone induced increases in antioxidant and eNOS gene expression lead to improved endothelial function and reduced blood pressure in vivo. FASEB J 2005;19:1755-7.
19 Si H, Liu D. Genistein, a soy phytoestrogen, upregulates the expression of human endothelial nitric oxide synthase and lowers blood pressure in spontaneously hypertensive rats. J Nutr 2008;138:297-304.
20 Rimbach G, Boesch-Saadatmandi C, Frank J, Fuchs D, Wenzel U, Daniel H, Hall WL, Weinberg PD. Dietary isoflavones in the prevention of cardiovascular disease--a molecular perspective. Food Chem Toxicol 2008;46:1308-19.   DOI   ScienceOn
21 Berliner JA, Navab M, Fogelman AM, Frank JS, Demer LL, Edwards PA, Watson AD, Lusis AJ. Atherosclerosis: basic mechanisms. Oxidation, inflammation, and genetics. Circulation 1995;91:2488-96.   DOI   ScienceOn
22 Bowie AG, Moynagh PN, O'Neill LA. Lipid peroxidation is involved in the activation of NF-${\kappa}B$ by tumor necrosis factor but not interleukin-1 in the human endothelial cell line ECV304. Lack of involvement of H2O2 in NF-${\kappa}B$ activation by either cytokine in both primary and transformed endothelial cells. J Biol Chem 1997;272:25941-50.   DOI
23 May MJ, Wheeler-Jones CP, Pearson JD. Effects of protein tyrosine kinase inhibitors on cytokine-induced adhesion molecule expression by human umbilical vein endothelial cells. Br J Pharmacol 1996;118:1761-71.   DOI   ScienceOn
24 Woodman OL, Missen MA, Boujaoude M. Daidzein and 17 beta-estradiol enhance nitric oxide synthase activity associated with an increase in calmodulin and a decrease in caveolin-1. J Cardiovasc Pharmacol 2004;44:155-63.   DOI
25 Kim DS, Kwon HM, Choi JS, Kang SW, Ji GE, Kang YH. Resveratrol blunts tumor necrosis factor-$\alpha$-induced monocyte adhesion and transmigration. Nutr Res Pract 2007;1:285-90.   DOI
26 Guo JM, Xiao BX, Liu DH, Grant M, Zhang S, Lai YF, Guo YB, Liu Q. Biphasic effect of daidzein on cell growth of human colon cancer cells. Food Chem Toxicol 2004;42:1641-6.   DOI   ScienceOn
27 Tetsuka T, Srivastava SK, Morrison AR. Tyrosine kinase inhibitors, genistein and herbimycin A, do not block interleukin-1 beta-induced activation of NF-kappa B in rat mesangial cells. Biochem Biophys Res Commun 1996;218:808-12.   DOI   ScienceOn
28 Lee YW, Kuhn H, Hennig B, Neish AS, Toborek M. IL-4-induced oxidative stress upregulates VCAM-1 gene expression in human endothelial cells. J Mol Cell Cardiol 2001;33:83-94.   DOI   ScienceOn
29 Gottstein N, Ewins BA, Eccleston C, Hubbard GP, Kavanagh IC, Minihane AM, Weinberg PD, Rimbach G. Effect of genistein and daidzein on platelet aggregation and monocyte and endothelial function. Br J Nutr 2003;89:607-15.   DOI
30 Fautz R, Husein B, Hechenberger C. Application of the neutral red assay (NR assay) to monolayer cultures of primary hepatocytes: rapid colorimetric viability determination for the unscheduled DNA synthesis test (UDS). Mutat Res 1991;253:173-9.   DOI   ScienceOn
31 D'Agostino P, Ferlazzo V, Milano S, La Rosa M, Di Bella G, Caruso R, Barbera C, Grimaudo S, Tolomeo M, Feo S, Cillari E. Anti-inflammatory effects of chemically modified tetracyclines by the inhibition of nitric oxide and interleukin-12 synthesis in J774 cell line. Int Immunopharmacol 2001;1:1765-76.   DOI   ScienceOn
32 Dignam JD, Lebovitz RM, Roeder RG. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res 1983;11:1475-89.   DOI   ScienceOn
33 Bingham SA, Atkinson C, Liggins J, Bluck L, Coward A. Phytooestrogens: where are we now? Br J Nutr 1998;79:393-406.   DOI
34 Weber C, Negrescu E, Erl W, Pietsch A, Frankenberger M, Ziegler-Heitbrock HW, Siess W, Weber PC. Inhibitors of protein tyrosine kinase suppress TNF-stimulated induction of endothelial cell adhesion molecules. J Immunol 1995;155:445-51.
35 Libby P, Galis ZS. Cytokines regulate genes involved in atherogenesis. Ann N Y Acad Sci 1994;748:158-68.
36 Anthony MS, Clarkson TB, Williams JK. Effects of soy isoflavones on atherosclerosis: potential mechanisms. Am J Clin Nutr 1998;68:1390S-1393S.