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http://dx.doi.org/10.4162/nrp.2011.5.2.112

Effect of paternal folate deficiency on placental folate content and folate receptor ${\alpha}$ expression in rats  

Kim, Hye-Won (Department of Nutritional Science and Food Management, Ewha Womans University)
Choi, Yun-Jung (Department of Nutritional Science and Food Management, Ewha Womans University)
Kim, Ki-Nam (Department of Nutritional Science and Food Management, Ewha Womans University)
Tamura, Tsunenobu (Department of Nutritional Science and Food Management, Ewha Womans University)
Chang, Nam-Soo (Department of Nutritional Science and Food Management, Ewha Womans University)
Publication Information
Nutrition Research and Practice / v.5, no.2, 2011 , pp. 112-116 More about this Journal
Abstract
We investigated the effect of paternal folate status on folate content and expression of the folate transporter folate receptor ${\alpha}$ ($FR{\alpha}$) in rat placental tissues. Rats were mated after males were fed a diet containing 0 mg of folic acid/kg of diet (paternal folate-deficient, PD) or 8 mg folic acid/kg of diet (paternal folate-supplemented, PS) for 4 weeks. At 20 days of gestation, the litter size, placental weight, and fetal weight were measured, and placental folate content (n=8/group) and expression of $FR{\alpha}$ (n=10/group) were analyzed by microbiological assay and Western blot analysis, respectively. Although there was no difference observed in litter size or fetal weight, but significant reduction (10%) in the weight of the placenta was observed in the PD group compared to that in the PS group. In the PD group, placental folate content was significantly lower (by 35%), whereas $FR{\alpha}$ expression was higher (by 130%) compared to the PS group. Our results suggest that paternal folate status plays a critical role in regulating placental folate metabolism and transport.
Keywords
Paternal folate status; placental folate; placental weight; folate receptor alpha;
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