Browse > Article
http://dx.doi.org/10.4162/nrp.2010.4.3.196

Neuroprotective effects of consuming bovine colostrum after focal brain ischemia/reperfusion injury in rat model  

Choi, Han-Sung (Department of Emergency Medicine, College of Medicine, Kyung Hee University)
Ko, Young-Gwan (Department of Emergency Medicine, College of Medicine, Kyung Hee University)
Lee, Jong-Seok (Department of Emergency Medicine, College of Medicine, Kyung Hee University)
Kwon, Oh-Young (Department of Emergency Medicine, College of Medicine, Kyung Hee University)
Kim, Sun-Kyu (Department of Fermented Food Science, Seoul University of Venture & Information)
Cheong, Chul (Department of Fermented Food Science, Seoul University of Venture & Information)
Jang, Ki-Hyo (Department of Food and Nutrition, Kangwon National University)
Kang, Soon-Ah (Department of Fermented Food Science, Seoul University of Venture & Information)
Publication Information
Nutrition Research and Practice / v.4, no.3, 2010 , pp. 196-202 More about this Journal
Abstract
To investigate the neuroprotective effects of bovine colostrums (BC), we evaluate the ability of consuming BC after focal brain ischemia/reperfusion injury rat model to reduce serum cytokine levels and infarct volume, and improve neurological outcome. Sprague-Dawley rats were randomly divided into 4 groups; one sham operation and three experimental groups. In the experimental groups, MCA occlusion (2 h) and subsequent reperfusion (O/R) were induced with regional cerebral blood flow monitoring. One hour after MCAO/R and once daily during the experiment, the experimental group received BC while the other groups received 0.9% saline or low fat milk (LFM) orally. Seven days later, serum pro-inflammatory cytokine (IL-$1{\beta}$, IL-6, and TNF-${\alpha}$) and anti-inflammatory cytokine (IL-10) levels were assessed. Also, the infarct volume was assessed by using a computerized image analysis system. Behavioral function was also assessed using a modified neurologic severity score and corner turn test during the experiment. Rats receiving BC after focal brain I/R showed a significant reduction (-26%/-22%) in infarct volume compared to LFM/saline rats, respectively (P < 0.05). Serum IL-$1{\beta}$, IL-6, and TNF-${\alpha}$ levels were decreased significantly in rats receiving BC compared to LFM/saline rats (P < 0.05). In behavioral tests, daily BC intake showed consistent and significant improvement of neurological deficits for 7 days after MCAO/R. BC ingestion after focal brain ischemia/reperfusion injury may prevent brain injury by reducing serum pro-inflammatory cytokine levels and brain infarct volume in a rat model.
Keywords
Focal brain ischemia/reperfusion; bovine colostrum; cytokines; neuroprotection;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
연도 인용수 순위
1 Davalos A, Ricart W, Gonzalez-Huix F, Soler S, Marrugat J, Molins A, Suner R, Genis D. Effect of malnutrition after acute stroke on clinical outcome. Stroke 1996;27:1028-32.   DOI   ScienceOn
2 Kim KY, Kim MY, Choi HS, Jin BK, Kim SU, Lee YB. Thrombin induces IL-10 production in microglia as a negative feedback regulator of TNF-${\alpha}$ release. Neuroreport 2002;13:849-52.   DOI   ScienceOn
3 Iadecola C, Alexander M. Cerebral ischemia and inflammation. Curr Opin Neurol 2001;14:89-94.   DOI   ScienceOn
4 Lourbopoulos A, Karacostas D, Artemis N, Milonas I, Grigoriadis N. Effectiveness of a new modified intraluminal suture for temporary middle cerebral artery occlusion in rats of various weight. J Neurosci Methods 2008;173:225-34.   DOI   ScienceOn
5 Li TJ, Qiu Y, Mao JQ, Yang PY, Rui YC, Chen WS. Protective effects of Guizhi-Fuling-Capsules on rat brain ischemia/ reperfusion injury. J Pharmacol Sci 2007;105:34-40.   DOI   ScienceOn
6 Shohami E, Novikov M. Long-term effect of HU-211, a novel non-competitive NMDA antagonist, on motor and memory functions after closed head injury in the rat. Brain Res 1995; 674:55-62.   DOI   ScienceOn
7 Peroulis N, Kourounakis AP, Yiangou M, Paramythiotis D, Kotzampassi K, Hadjipetrou L. Effects of the novel non-steroidal anti-inflammatory compound [N-(2-Thiolethyl)-2-{2-[N'-(2,6-dichlorophenyl) amino]phenyl}acetamide on cytokines and apoptosis in ischaemic rat brain. Arzneimittelforschung 2006;56:688-94.
8 Hayashida K, Kaneko T, Takeuchi T, Shimizu H, Ando K, Harada E. Oral administration of lactoferrin inhibits inflammation and nociception in rat adjuvant-induced arthritis. J Vet Med Sci 2004;66:149-54.   DOI   ScienceOn
9 Li Y, Chen J, Chen XG, Wang L, Gautam SC, Xu YX, Katakowski M, Zhang LJ, Lu M, Janakiraman N, Chopp M. Human marrow stromal cell therapy for stroke in rat: neurotrophins and functional recovery. Neurology 2002;59:514-23.   DOI   ScienceOn
10 Zhang L, Schallert T, Zhang ZG, Jiang Q, Arniego P, Li Q, Lu M, Chopp M. A test for detecting long-term sensorimotor dysfunction in the mouse after focal cerebral ischemia. J Neurosci Methods 2002;117:207-14.   DOI   ScienceOn
11 Kelly GS. Bovine colostrums: a review of clinical uses. Altern Med Rev 2003;8:378-94.
12 Pajkrt D, van der Poll T, Levi M, Cutler DL, Affrime MB, van den Ende A, ten Cate JW, van Deventer SJ. Interleukin-10 inhibits activation of coagulation and fibrinolysis during human endotoxemia. Blood 1997;89:2701-5.
13 Scumpia PO, Moldawer LL. Biology of interleukin-10 and its regulatory roles in sepsis syndromes. Crit Care Med 2005;33: S468-71.   DOI   ScienceOn
14 Quigley JD 3rd, Drewry JJ. Nutrient and immunity transfer from cow to calf pre- and post-calving. J Dairy Sci 1998;81:2779-90.   DOI   ScienceOn
15 Goto M, Maruyama M, Kitadate K, Kirisawa R, Obata Y, Koiwa M, Iwai H. Detection of nterleukin-1 beta in sera and colostrum of dairy cattle and in sera of neonates. J Vet Med Sci 1997;59: 437-41.   DOI   ScienceOn
16 Hagiwara K, Kataoka S, Yamanaka H, Kirisawa R, Iwai H. Detection of cytokines in bovine colostrum. Vet Immunol Immunopathol 2000;76:183-90.   DOI   ScienceOn
17 Choi HS, Jung KH, Lee SC, Yim SV, Chung JH, Kim YW, Jeon WK, Hong HP, Ko YG, Kim CH, Jang KH, Kang SA. Bovine colostrum prevents bacterial translocation in an intestinal ischemia/reperfusion-injured rat model. J Med Food 2009;12:37-46.   DOI   ScienceOn
18 Chun S, Nam M, Goh J, Kim W, Han Y, Kim P. Kinetics and biological function of transforming growth factor-${\beta}$ isoforms in bovine and human colostrum. J Microbiol Biotechnol 2004;14: 1267-74.
19 Playford RJ. Peptide therapy and the gastroenterologist: colostrum and milk-derived growth factors. Clin Nutr 2001;20:101-6.
20 Playford RJ, Floyd DN, Macdonald CE, Calnan DP, Adenekan RO, Johnson W, Goodlad RA, Marchbank T. Bovine colostrum is a health food supplement which prevents NSAID induced gut damage. Gut 1999;44:653-8.   DOI   ScienceOn
21 Sun H, Zhao H, Sharpe GM, Arrick DM, Mayhan WG. Effect of chronic alcohol consumption on brain damage following transient focal ischemia. Brain Res 2008;1194:73-80.   DOI   ScienceOn
22 Zhang Y, Wang L, Li J, Wang XL. 2-(1-Hydroxypentyl)-benzoate increases cerebral blood flow and reduces infarct volume in rats model of transient focal cerebral ischemia. J Pharmacol Exp Ther 2006;317:973-9.   DOI   ScienceOn
23 An MJ, Cheon JH, Kim SW, Park JJ, Moon CM, Han SY, Kim ES, Kim TI, Kim WH. Bovine colostrum inhibits nuclear factor ${\kappa}$B-mediated proinflammatory cytokine expression in intestinal epithelial cells. Nutr Res 2009;29:275-80.   DOI   ScienceOn
24 Filipp D, Alizadeh-Khiavi K, Richardson C, Palma A, Paredes N, Takeuchi O, Akira S, Julius M. Soluble CD14 enriched in colostrum and milk induces B cell growth and differentiation. Proc Natl Acad Sci U S A 2001;98:603-8.   DOI   ScienceOn
25 Hagiwara K, Kitajima K, Yamanaka H, Kirisawa R, Iwai H. Development of a sandwich ELISA assay for measuring bovine soluble type II IL-1 receptor (IL1R2) concentration in serum and milk. Cytokine 2005;32:132-6.   DOI   ScienceOn
26 Shing CM, Peake JM, Suzuki K, Jenkins DG, Coombes JS. Bovine colostrum modulates cytokine production in human peripheral blood mononuclear cells stimulated with lipopolysaccharide and phytohemagglutinin. J Interferon Cytokine Res 2009;29:37-44.   DOI   ScienceOn
27 Longa EZ, Weinstein PR, Carlson S, Cummins R. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 1989;20:84-91.   DOI   ScienceOn
28 Chen H, Song YS, Chan PH. Inhibition of NADPH oxidase is neuroprotective after ischemia-reperfusion. J Cereb Blood Flow Metab 2009;29:1262-72.   DOI   ScienceOn
29 Swanson RA, Morton MT, Tsao-Wu G, Savalos RA, Davidson C, Sharp FR. A semiautomated method for measuring brain infarct volume. J Cereb Blood Flow Metab 1990;10:290-3.   DOI
30 Chen J, Li Y, Wang L, Zhang Z, Lu D, Lu M, Chopp M. Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats. Stroke 2001;32: 1005-11.   DOI   ScienceOn
31 The National Institute of Neurological Disorders and Stroke rt-PA Stroke Study Group. Tissue plasminogen activator for acute ischemic stroke. N Engl J Med 1995;333:1581-7.   DOI   ScienceOn
32 Green AR, Shuaib A. Therapeutic strategies for the treatment of stroke. Drug Discov Today 2006;11:681-93.   DOI   ScienceOn
33 Feigin VL, Lawes CM, Bennett DA, Anderson CS. Stroke epidemiology: a review of population-based studies of incidence, prevalence, and case-fatality in the late 20th century. Lancet Neurol 2003;2:43-53.   DOI   ScienceOn
34 Plaisier F, Bastide M, Ouk T, Petrault O, Laprais M, Stolc S, Bordet R. Stobadine-induced hastening of sensorimotor recovery after focal ischemia/reperfusion is associated with cerebrovascular protection. Brain Res 2008;1208:240-9.   DOI   ScienceOn
35 Allan SM, Rothwell NJ. Cytokines and acute neurodegeneration. Nat Rev Neurosci 2001;2:734-44.   DOI   ScienceOn
36 Haelewyn B, Freret T, Pacary E, Schumann-Bard P, Boulouard M, Bernaudin M, Bouet V. Long-term evaluation of sensorimotor and mnesic behaviour following striatal NMDA-induced unilateral excitotoxic lesion in the mouse. Behav Brain Res 2007;178: 235-43.   DOI   ScienceOn
37 Lee DH, Ha N, Bu YM, Choi HI, Park YG, Kim YB, Kim MY, Kim H. Neuroprotective effect of Buddleja officinalis extract on transient middle cerebral artery occlusion in rats. Biol Pharm Bull 2006;29:1608-12.   DOI   ScienceOn
38 del Zoppo G, Ginis I, Hallenbeck JM, Iadecola C, Wang X, Feuerstein GZ. Inflammation and stroke: putative role for cytokines, adhesion molecules and iNOS in brain response to ischemia. Brain Pathol 2000;10:95-112.
39 Dienz O, Rincon M. The effects of IL-6 on CD4 T cell responses. Clin Immunol 2009;130:27-33.   DOI   ScienceOn
40 Dinarello CA. Proinflammatory cytokines. Chest 2000;118;503-8.   DOI   ScienceOn
41 Hallenbeck JM, Dutka AJ. Background review and current concepts of reperfusion injury. Arch Neurol 1990;47:1245-54.   DOI   ScienceOn
42 Xiao ZY, Sun CK, Xiao XW, Lin YZ, Li S, Ma H, Song GR, Cheng R. Effects of Ginkgo biloba extract against excitotoxicity induced by NMDA receptors and mechanism thereof. Zhonghua Yi Xue Za Zhi 2006;86:2479-84.
43 Saleem S, Zhuang H, Biswal S, Christen Y, Dore S. Ginkgo Biloba extract neuroprotective action is dependent on heme oxygenase 1 in ischemic reperfusion brain injury. Stroke 2008;39:3389-96.   DOI   ScienceOn
44 Chan PH. Reactive oxygen radicals in signaling and damage in the ischemic brain. J Cereb Blood Flow Metab 2001;21:2-14.   DOI
45 Fisher M. Characterizing the target of acute stroke therapy. Stroke 1997;28:866-72.   DOI   ScienceOn