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http://dx.doi.org/10.4014/jmb.1706.06057

Delivery of Chicken Egg Ovalbumin to Dendritic Cells by Listeriolysin O-Secreting Vegetative Bacillus subtilis  

Roeske, Katarzyna (Department of Applied Microbiology, Faculty of Biology, University of Warsaw)
Stachowiak, Radoslaw (Department of Applied Microbiology, Faculty of Biology, University of Warsaw)
Jagielski, Tomasz (Department of Applied Microbiology, Faculty of Biology, University of Warsaw)
Kaminski, Michal (Department of Applied Microbiology, Faculty of Biology, University of Warsaw)
Bielecki, Jacek (Department of Applied Microbiology, Faculty of Biology, University of Warsaw)
Publication Information
Journal of Microbiology and Biotechnology / v.28, no.1, 2018 , pp. 122-135 More about this Journal
Abstract
Listeriolysin O (LLO), one of the most immunogenic proteins of Listeria monocytogenes and its main virulence factor, mediates bacterial escape from the phagosome of the infected cell. Thus, its expression in a nonpathogenic bacterial host may enable effective delivery of heterologous antigens to the host cell cytosol and lead to their processing predominantly through the cytosolic MHC class I presentation pathway. The aim of this project was to characterize the delivery of a model antigen, chicken egg ovalbumin (OVA), to the cytosol of dendritic cells by recombinant Bacillus subtilis vegetative cells expressing LLO. Our work indicated that LLO produced by non-sporulating vegetative bacteria was able to support OVA epitope presentation by MHC I molecules on the surface of antigen presenting cells and consequently influence OVA-specific cytotoxic T cell activation. Additionally, it was proven that the genetic context of the epitope sequence is of great importance, as only the native full-sequence OVA fused to the N-terminal fragment of LLO was sufficient for effective epitope delivery and activation of $CD8^+$ lymphocytes. These results demonstrate the necessity for further verification of the fusion antigen potency of enhancing the MHC I presentation, and they prove that LLO-producing B. subtilis may represent a novel and attractive candidate for a vaccine vector.
Keywords
Antigen delivery; Bacillus subtilis; cellular response; listeriolysin O; vaccine design;
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