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http://dx.doi.org/10.3746/pnf.2013.18.2.124

Hepatic Fibrosis Inhibitory Effect of Peptides Isolated from Navicula incerta on TGF-β Induced Activation of LX-2 Human Hepatic Stellate Cells  

Kang, Kyong-Hwa (Marine Bioprocess Research Center, Pukyong National University)
Qian, Zhong-Ji (Oceanic Life Research Center, Chosun University)
Ryu, BoMi (Marine Bioprocess Research Center, Pukyong National University)
Karadeniz, Fatih (Marine Bioprocess Research Center, Pukyong National University)
Kim, Daekyung (Marine Bio Research Team, Korea Basic Science Institute (KBSI))
Kim, Se-Kwon (Marine Bioprocess Research Center, Pukyong National University)
Publication Information
Preventive Nutrition and Food Science / v.18, no.2, 2013 , pp. 124-132 More about this Journal
Abstract
In this study, novel peptides (NIPP-1, NIPP-2) derived from Navicula incerta (microalgae) protein hydrolysate were explored for their inhibitory effects on collagen release in hepatic fibrosis with the investigation of its underlying mechanism of action. TGF-${\beta}1$ activated fibrosis in LX-2 cells was examined in the presence or absence of purified peptides NIPP-1 and NIPP-2. Besides the mechanisms of liver cell injury, protective effects of NIPP-1 and NIPP-2 were studied to show the protective mechanism against TGF-${\beta}1$ stimulated fibrogenesis. Our results showed that the core protein of NIPP-1 peptide prevented fibril formation of type I collagen, elevated the MMP level and inhibited TIMP production in a dose-dependent manner. The treatment of NIPP-1 and NIPP-2 on TGF-${\beta}1$ induced LX-2 cells alleviated hepatic fibrosis. Moreover, ${\alpha}$-SMA, TIMPs, collagen and PDGF in the NIPP-1 treated groups were significantly decreased. Therefore, it could be suggested that NIPP-1 has potential to be used in anti-fibrosis treatment.
Keywords
microalgae; hepatic stellate cells; hepatic fibrosis; LX-2; liver injury;
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