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http://dx.doi.org/10.3746/jfn.2011.16.4.299

Anti-Inflammatory Effect of Fermented Liriope platyphylla Extract in LPS-stimulated RAW 264.7 Macrophages  

Lee, Hyun-Ah (Department of Food Science and Nutrition, Pusan National University)
Han, Ji-Sook (Department of Food Science and Nutrition, Pusan National University)
Publication Information
Preventive Nutrition and Food Science / v.16, no.4, 2011 , pp. 299-306 More about this Journal
Abstract
The present study was designed to evaluate the inhibitory effects of fermented Liriope platyphylla extract on the production of inflammation-related mediators (NO, ROS, NF-${\kappa}B$, iNOS and COX-2) and pro-inflammatory cytokines (TNF-${\alpha}$, IL-$1{\beta}$, IL-6) in lipopolysaccharide-stimulated RAW 264.7 macrophages. Freeze-dried Liriope platyphylla was fermented by Saccharomyces cerevisiae and extracted with 70% ethanol. In lipopolysaccharide-stimulated macrophage cells, the treatment with fermented Liriope platyphylla extract decreased the generation of intracellular reactive oxygen species dose-dependently and increased antioxidant enzyme activities, including superoxide dismutase, catalase and glutathione peroxidase. Fermented Liriope platyphylla extract also inhibited NO production in lipopolysaccharide-stimulated RAW 264.7 cell. The expressions of NF-${\kappa}B$, iNOS, COX-2 and pro-inflammatory cytokines were inhibited by the treatment with fermented Liriope platyphylla extract. Thus, this study shows the fermented Liriope platyphylla extract could be effective at inhibiting the inflammation process.
Keywords
fermented Liriope platyphylla; anti-inflammation; lipopolysaccharide; RAW 264.7 cell;
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1 Alderton WK, Cooper CE, Knowles RG. 2001. Nitric oxide synthases: structure, function and inhibition. Biochem J 357: 593-615.   DOI
2 Gaestel M, Mengel A, Bothe U, Asadullah K. 2007. Protein kinases as small molecule inhibitor targets in inflammation. Cur Med Chem 14: 2214-2234.   DOI   ScienceOn
3 Vane JR, Mitchell JA, Appleton I. 1994. Inducible isoforms of cyclooxygenase and nitric oxide synthase in inflammation. Proc Natl Acad Sci 91: 2046-2050.   DOI
4 Swierkoza TA, Mitchell JA, Warner TD, Botting RM, Vane JR. 1995. Co-induction of nitric oxide synthase and cyclooxygenase: Interaction between nitric oxide and prostanoids. Br J Pharmacol 114: 1335-1342.   DOI   ScienceOn
5 Conforti F, Sosa S, Marrelli M, Menichini F, Statti GA, Uzunov D, Tubaro A, F Menichini. 2009. The protective ability of Mediterranean dietary plants against the oxidative damage: the role of radical oxygen species in inflammation and the polyphenol, flavonoid and sterol contents. Food Chem 112: 587-594.   DOI   ScienceOn
6 Yasui K, Baba A. 2006. Therapeutic potential of superoxide dismutase for resolution of inflammation. Inflamm Res 55: 359-363.   DOI
7 Benhamou PY, Moriscot C, Richard MJ. 1998. Adenovirusmediated catalase gene transcription reduces oxidant stress in human, porcine and rat pancreatic islets. Diabetologia 41: 1093-1100.   DOI
8 Marsh CB, Wewers MD. 1996. The pathogenesis of sepsis: factors that modulate the response to gram-negative bacterial infection. Clin Chest Med 17: 183-197.   DOI
9 Munhoz CD, Garcia-Bueno B, Madrigal JLM, Lepsch LB, Scavone C, Leza JC. 2008. Stress-induced neuroinflammation: mechanisms and new pharmacological targets. Bruz J Med Biol Res 41: 1037-1046.   DOI
10 Li XA, Everson W, Smart EJ. 2006. Nitric oxide, caveolae and vascular pathology. Cardiovasc Toxicol 6: 1-13.   DOI   ScienceOn
11 Aggarwal BB, Natarajan K. 1996. Tumor necrosis factors: developments during the last decade. Eur Cytokine Network 7: 93-124.
12 Kim JY, Park SJ, Yun KJ, Cho YW, Park HJ, Lee KT. 2008. Isoliquiritigenin isolated from the roots of Glycyrrhiza uralensis inhibits LPS-induced iNOS and COX-2 expression via the attenuation of NF-kB in RAW 264.7 macrophages. Eur J Pharmacol 584: 175-184.   DOI   ScienceOn
13 Lappas M, Permezel M, Georgiou HM, Rice GE. 2002. Nuclear factor kappa B regulation of proinflammatory cytokines in human gestational tissues in vitro. Biol Reprod 67: 668-673.   DOI   ScienceOn
14 Bradford MM. 1976. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Ann Biochem 72: 248-254.   DOI   ScienceOn
15 Aebi H. 1984. Catalase in vitro. Methods Enzymol 105: 121-126.   DOI
16 Lawrence RA, Burk RF. 1976. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 71: 952-958.   DOI   ScienceOn
17 D'Agostino P, Ferlazzo V, Milano S, La Rosa M, Di Bella G, Caruso R, Barbera C, Grimaudo S, Tolomeo M, Feo S, Cillari E. 2001. Anti-inflammatory effects of chemically modified tetracyclines by the inhibition of nitric oxide and inteleukin-12 synthesis in J774 cell line. Int Immunopharmacol 1: 1765-1776.   DOI   ScienceOn
18 Kim EK. 2008. Purification and characterization of antioxidative peptides from enzymatic hydrolysates of venison. PhD Dissertation. Pusan National University, Busan, Korea.
19 Koppenaol WH, Liebman JF. 1984. The oxidizing nature of the hydroxyl radicals: A comparison with the ferryl ion. J Phys Chem 88: 99-101.   DOI
20 Lui F, Ng TB. 1999. Antioxidative and free radical scavenging activities of selected medicinal herbs. J Life Sci 66: 725-735.
21 Hu CC, Hsiao CH, Huang SY, Fu SH, Lai CC, Hong TM, Chen HH, Lu FJ. 2004. Antioxidant activity of fermented soybean extract. J Agric Food Chem 52: 5735-5739.   DOI   ScienceOn
22 Bae HN, Kim YM. 2010. Improvement of the functional qualities of sea tangle extract through fermentation by Aspergillus Oryzae. Fish Aqua Sci 13: 12-17.
23 Elaine MC, Matthew BG. 1996. Inflammation, free radicals and antioxidants. Nutrition 12: 274-277.   DOI   ScienceOn
24 Gloire GL-PS, Piette J. 2006. NF-kappa B activation by reactive oxygen species: fifteen years later. Biochem Pharmacol 72: 1493-1505.   DOI   ScienceOn
25 Choi SB, Wha JD, Park S. 2004. The insulin sensitizing effect of homoisoflavone-enriched fraction in Liriope platyphylla Wang et Tang via PI3-kinase pathway. Life Sci 75: 2653-2664.   DOI   ScienceOn
26 Shibata M, Noguchi R, Suzuki M, Iwase H, Soeda K, Niwayama K, Kataoke E, Hamano M. 1971. Pharmacological studies on medicinal plant components I. On the extracts of Ophiopohon and some folk medicine. Proc Hoshi Pharm 13: 66-76.
27 Yu BY, Qui SX, Zaw K, Xu GJ, Hirai Y, Shoji J, Fong HH, Kinghorn AD. 1996. Steroidal glycosides from the subterranean parts of Liriope spicata var. prolifera. Phytochemistry 43: 201-206.   DOI   ScienceOn
28 Yamaguchi T, Takamura H, Matoba T, Terao J. 1998. HPLC method for evaluation of the free radical-scavenging activity of food by using 1,1-diphenyl-2-picrylhydrazyl. Biotechnol Biochem 62: 1201-1204.   DOI   ScienceOn
29 Chung SK, Osawa T, Kawakishi S. 1997. Hydroxyl radical- scavenging effects and scavengers from brown mustard (Bassica niger). Biotech Biochem 61: 118-123.   DOI   ScienceOn
30 Kirby AJ, Schmidt RJ. 1997. The antioxidant activity of Chinese herbs for eczema and placebo herbs-I. J Ethnopharmacol 56: 103-108.   DOI   ScienceOn
31 Mitsuda H, Yasumoto K, Iwama K. 1996. Antioxidative action of indole compounds during the autoxidation of linoleic acid. Eiyo Shokuryo 19: 210-214.
32 Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxicity assays. J Immunol Methods 65: 55-63.   DOI   ScienceOn
33 Leloup C, Magnan C, Benani A, Bonnet E, Alquier T, Offer G, Carriere A, Periquet A, Fernandez Y, Ktorza A, Casteilla L, Penicaud L. 2006. Mitochondrial reactive oxygen species are required for hypothalamic glucose sensing. Diabetes 55: 2084-2090.   DOI   ScienceOn
34 Mantovani A, Allavena P, Sica A, Balkwill F. 2008. Cancer-related inflammation. Nature 454: 436-444.   DOI   ScienceOn
35 Hsu HY, Wen MH. 2002. Lipopolysaccharide-mediated reactive oxygen species and signal transduction in the regulation of interleukin-1 gene expression. J Biol Chem 277: 22131-22139.   DOI   ScienceOn
36 Gordon S. 2003. Alternative activation of macrophges. Nat Res Immunol 3: 23-35.   DOI   ScienceOn
37 Chung WY, Part JH, Kim MJ, Kim HO, Hwang JK, Lee SK. 2007. Xanthorrhizol inhibits 12-O-tetradecanoylphorbol-13-acetate-induced acute inflammation and two-stage mouse skin carcinogenesis by blocking the expression of ornithine decarboxylase, cyclooxygenase-2 and inducible nitric oxide synthase through mitogen-activated protein kinases and/or the nuclear factor-${\kappa}B$. Carcinogenesis 28: 1224-1231.   DOI   ScienceOn
38 Murakama A. 2009. Chemoprevention with phytochemicals targeting inducibles nitric oxide synthase. Food factors for health promotion. Forum of Nutrition Basel Karger 61: 193-203.   DOI   ScienceOn
39 Kabe Y, Ando K, Hirao S, Yoshida M, Handa H. 2005. Redox regulation of inflammatory processes. Mem Inst Oswaldo Cruz 100: 5-9.   DOI
40 Baeuerla PA, Baltimore D. 1996. NF-kappa B: Ten years after. Cell 87: 13-20.   DOI   ScienceOn
41 Hur J, Lee P, Kim J, Kim AJ, Kim H, Kim SY. 2004. Induction of nerve growth factor by butanol fraction of Liriope platyphylla in C6 and primary astrocyte cells. Biol Pharm Bull 27: 1257-1260.   DOI   ScienceOn