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http://dx.doi.org/10.3746/jfn.2008.13.4.313

Effect of Far-infrared Radiation for Dying Citrus By-products and Their Radical Scavenging Activities and Protective Effects Against H2O2-induced DNA Damage  

Senevirathne, Mahinda (Department of Food Bioengineering, Cheju National University)
Jeon, You-Jin (Faculty of Applied Marine Science, Cheju National University)
Ha, Jin-Hwan (Department of Food Bioengineering, Cheju National University)
Kim, Soo-Hyun (Department of Food Bioengineering, Cheju National University)
Publication Information
Preventive Nutrition and Food Science / v.13, no.4, 2008 , pp. 313-320 More about this Journal
Abstract
Efficiency of a far-infrared radiation (FIR) dryer for drying of citrus by-products (CBPs) was evaluated through their antioxidant activities. The CBPs dried through FIR were enzymatically digested by six carbohydrases (AMG, Celluclast, Pectinase, Termamyl, Ultraflo and Viscozyme) to prepare digests for evaluation of the activities. The total polyphenolic and total flavonoid contents of the digests were determined by colorimetric assays. The AMG digest was selected for the further experiments. The antioxidant potential of the digests were evaluated by DPPH, superoxide, hydroxyl and alkyl radical scavenging activities, $H_2O_2$ scavenging activity, metal chelating, lipid peroxidation inhibition and the reduction of DNA damage. The AMG digest from CBPs dried through FIR at $50^{\circ}C$ showed strong antioxidant activities in DPPH, superoxide, hydrogen peroxide, alkyl and metal chelating assays while all the digests showed strong lipid peroxidation activities. Further, enzymatic digests showed remarkable inhibitory activities against $H_2O_2$-induced DNA damage. Hence, the data obtained using different in vitro models clearly established the antioxidant potential of enzymatic digests from CBPs dried through FIR. Furthermore, they can be used as a source of natural antioxidants; hence, far-infrared radiation drying is a viable method for transforming wet CBPs into a dried form without destroying the bioactive components.
Keywords
antioxidant activity; citrus by-products; DNA damage; enzymatic digests; far-infrared radiation drying;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Havsteen B. 1983. Biochemical effects of flavonoids. Biochem Pharmacol 32: 1141-1148   DOI   ScienceOn
2 Middleton EJ, Kandaswami C. 1994. Potential health promoting properties of citrus flavonoids. Food Technol 18: 115-120
3 Kim JW, Jeon YJ, Lee JH, Lee SC. 2006. Effect of far-infrared irradiation and heat treatment on the antioxidant activity of extracts from citrus pomaces. J Korean Soc Appl Biol Chem 49: 60-64   과학기술학회마을
4 Guo Q, Zhao B, Shen S, Hou J, Hu J, Xin W. 1999. ESR study on the structure-antioxidant activity relationship of tea catechins and their epimers. Biochem Biophy Acta 1427: 13-23   DOI   ScienceOn
5 Hemmerich P, Wessiak A. 1976. The structural chemistry of flavin-dependent oxygen activation. Proceedings of the fifth international symposium, flavins and flavoproteins. Singer TP, ed. Elsevier, Amsterdam. p 9-22
6 Cook NC, Samman S. 1996. Flavonoids-chemistry, metabolism, cardioprotective effects and dietary sources. Nutr Biochem 7: 66-76   DOI   ScienceOn
7 Huang D, Ou B, Prior RL. 2005 The chemistry behind antioxidant capacity assays. J Agric Food Chem 53: 1841-1856   DOI   ScienceOn
8 Cao G, Sofic E, Prior RL. 1996. Antioxidant capacity of tea and common vegetables. J Agric Food Chem 44: 3426-3431   DOI   ScienceOn
9 Wang H, Cao G, Prior RL. 1996. Total antioxidant capacity of fruits. J Agric Food Chem 44: 701-705   DOI   ScienceOn
10 Nakayama T. 1994. Suppression of hydroxyperoxide-induced cytotoxicity by polyphenols. Cancer Res 54: 1991-1993
11 Dreher D, Junod AF. 1996. Role of oxygen free radical in cancer development. Eur J Cancer 32: 30-35   DOI   ScienceOn
12 Masamura A, Sado H, Honda T, Shimizu M, Nabethani H, Hakajima M. 1988. Drying of potato by far infrared radiation. Nippon Shokuhin Kogyo Gakkaishi 35: 309-314   DOI
13 Chawla SP, Jo C, Kang HJ, Kim MJ, Byun MW. 2003. Bioactivities of citrus (Citrus unshui) peel extract subjected to different extraction conditions, storage temperatures and irradiation. J Food Sci Nutr 8: 349-355   과학기술학회마을   DOI   ScienceOn
14 Niwa Y, Miyachi Y. 1986. Antioxidant action of natural health products and Chinese herbs. Inflammation 10: 79-97   DOI
15 Jeong SM, Kim SY, Kim DR, Jo SC, Nam KC, Ahn DU, Lee SC. 2004. Effect of heat treatment on the antioxidant activity of extracts from citrus peels. J Agric Food Chem 52: 3389-3393   DOI   ScienceOn
16 Jo C, Lee JW, Byun MW. 2001. Short communication of novel application of food irradiation. J Food Sci Nutr 6: 253-256   과학기술학회마을
17 Ginzburg AS. 1969. Application of Infra-red Radiation in Food Processing. Leonard Hill, London. p 353-367
18 Peleg H, Naim M, Rouseff RL, Zehavi U. 1991. Distribution of bound and free phenolic acids in oranges (Citrus sinensis) and grapefruit (Citrus paradise). J Sci Food Agric 57: 417-426   DOI
19 Heo SJ, Lee KW, Song CB, Jeon YJ. 2003. Antioxidant activity of enzymatic extracts from brown seaweeds. Algae 18: 71-81   DOI   ScienceOn
20 Chandler SF, Dodds JH. 1993. The effect of phosphate, nitrogen and sucrose on the production of phenolics and solasidine in callus cultures of Solanum laciniatum. Plan Cell Reports 2: 105-110
21 Nanjo F, Goto K, Seto R, Suzuki H, Sakai M, Hara Y. 1996. Scavenging effect of tea catechin and their derivatives on 1,1-diphenyl-2-picrylhydrazyl radical. Free Radical Biol Med 21: 885-902   DOI   ScienceOn
22 Slupphaug G, Kavil B, Krokan HE. 2003. The interacting pathways for prevention and repair of oxidative DNA damage. Mutat Res-Fund Mol Mech Mutag 531: 231-251   DOI   ScienceOn
23 Decker EA, Welch B. 1990. Role of ferritin as a lipid oxidation catalyst in muscle food. J Agric Food Chem 38: 674-677   DOI
24 Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. 2006. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 160: 1-40   DOI   ScienceOn
25 Rosen GM, Rauckman EJ. 1980. Spin trapping of the primary radical involved in the activation of the carcinogen N-hydroxyl-2-aacetylaminoflurene by cumene hydroperoxide hematin. Mol Pham 17: 233-238
26 Muller HE. 1995. Detection of hydrogen peroxide produced by microorganism on ABTS-peroxidase medium. Zentralbl Bakteriol. Mikrobiol Hyg 259: 151-158
27 Osawa T, Namiki M. 1985. Natural antioxidants isolated from eucalyptus leaf waxes. J Agric Food Chem 33: 777-780   DOI
28 AOAC. 1995. Officical Methods of Analysis. 15 ed. Association of Official Anlytical Chemist, Washington, DC
29 Bondet V, Williams WB, Berset C. 1997. Kinetic and mechanism of antioxidant activity using the DPPH free radical method. Lebensmittel-Wissenschaft Un Technologie 30: 609-615   DOI   ScienceOn
30 Yi ZB, Yu Y, Liang YZ, Zeng B. 2008. In vitro antioxidant and antimicrobial activities of the extract of Pericarpium Citri Reticulatae of a new citrus cultivar and its main flavonoids. Food Sci Technol 41: 597-603   DOI   ScienceOn
31 Nakayama T, Yamaden M, Osawa T, Kawakishi S. 1993. Suppression of active oxygen-induced cytotoxicity by flavonoids. Biochem Pharmacol 45: 265-267   DOI   ScienceOn
32 Hiramoto K, Johkoh H, Sako K, Kikugawa K. 1993. DNA breaking activity of the carbon-centered radical generated from 2,2-azobis (2-amidinopropane) hydrochloride (AAPH). Free Rad Res Commun 19: 323-332   DOI
33 Prior RL, Wu X, Schaich K. 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 53: 4290-4302   DOI   ScienceOn
34 Jeong SM, Kim SY, Park HR, Lee SC. 2004. Effect of far-infrared radiation on the activity of extracts from Citrus unshiu peels. J Korean Soc Food Sci Nutr 33: 1580-1583   과학기술학회마을   DOI   ScienceOn
35 Zhuang XP, Lu YY, Yang GS. 1992. Extraction and determination of flavonoid in ginkgo. Chinese Herbal Med 23: 122-124
36 Singh NP, McCoy MT, Tice RR, Schneider EL. 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175: 184-191   DOI   ScienceOn
37 Jayaprakasha GK, Patil BS. 2007. In vitro evaluation of the antioxidant activities in fruit extracts from citron and blood orange. Food Chem 101: 410-418   DOI   ScienceOn
38 Lin SD, Liu EH, Mau JL. 2008. Effect of different brewing methods on antioxidant properties on steaming green tea. Food Sci Technol 41: 1616-1623   DOI   ScienceOn
39 Anagnostopoulou MA, Kefalas P, Papageorgiou VP, Assimopoulou AN, Boskou D. 2006. Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chem 94: 19-25   DOI   ScienceOn
40 Fukumoto LR, Mazza G. 2000. Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem 48: 597-3604   DOI   ScienceOn
41 TakaoT, Kitatani F, Watanabe N, Yagi A, Sakata K. 1994. A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biotech Biochem 58: 1780-1783   DOI
42 Arts IC, Hollman PC, Feskens EJ. 2001. Catechin intake might explain the inverse relation between tea consumption and ischemic heart disease: The Zutphen Elderly Study. Am J Clinical Nutr 74: 227-232   DOI
43 Takabe W, Niki E, Uchida K, Yamada S, Satoh K, Noguchi N. 2001. Oxidative stress promotes the development of transformation: Involvement of a potent mutagenic lipid peroxidation product acrolein. Carcinogenesis 22: 935-941   DOI   ScienceOn
44 Collins AR. 2004. The comet assay for DNA damage and repair: principles, applications, and limitations. Mol Biotechnol 26: 249-261   DOI   ScienceOn
45 Ratnam DV, Ankola DD, Bhardwaj V, Sahana DK, Kumar MN. 2006. Role of antioxidants in prophylaxis and therapy: a pharmaceutical perspective. J Control Release 113: 189-207   DOI   ScienceOn
46 Dziedzic SZ, Hudson BJF. 1983. Hydroxy isoflavones as antioxidants for edible oils. Food Chem 11: 161-166   DOI   ScienceOn
47 Cheng Z, Ren J, Li Y, Chang W, Chen Z. 2003. Establishment of a quantitative structure-activity relationship model for evaluating and predicting the protective potentials of phenolic antioxidants on lipid peroxidation. J Pharm Sci 92: 475-484   DOI   ScienceOn
48 Takahama U. 1983. Redox reactions between kaempferol and illuminated chloroplasts. Plant Physiol 71: 598-601   DOI   ScienceOn