Browse > Article
http://dx.doi.org/10.3746/jfn.2005.10.1.046

Protective Effect of Acanthopanax senticosus Extract on Alloxan-induced β-cell Damage  

Rho, Hye-Won (Department of Biochemistry, Medical School and Chonbuk National University, Institute of Cardiovascular Research, Chonbuk National University)
Lee, Ji-Hyun (Department of Biochemistry, Medical School and Chonbuk National University)
Kim, Jong-Suk (Department of Biochemistry, Medical School and Chonbuk National University)
Kim, Hyung-Rho (Department of Biochemistry, Medical School and Chonbuk National University)
Park, Byung-Hyun (Department of Biochemistry, Medical School and Chonbuk National University)
Park, Jin-Woo (Department of Biochemistry, Medical School and Chonbuk National University)
Publication Information
Preventive Nutrition and Food Science / v.10, no.1, 2005 , pp. 46-51 More about this Journal
Abstract
The protective effect of Acanthopanax senticosus (AS) extract on alloxan-induced pancreatic β-cell damage was investigated in HIT T-15 cells, a Syrian hamster pancreatic β-cell line. Alloxan caused the pancreatic β-cell damage through the generation of reactive oxygen free radicals, increased DNA fragmentation, and decreased cellular NAD/sup +/ levels. The β-cell damage was significantly prevented by the pretreatment with water soluble extract of AS roots. These results suggest that the protective effect of AS extract, on alloxan-induced β-cell damage, is primarily due to the inhibition of the generation of reactive oxygen free radical species (ROS) by alloxan.
Keywords
Acanthopanax senticosus (AS); protective effect; alloxan; oxygen free radicals;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Heikkila RE, Winston B, Cohen G, Barden H. 1976. Alloxan-induced diabetes-evidence for hydroxyl radical as a cytotoxic intermediate. Biochem Pharmacol 25: 1985-1092
2 Cohen G, Heikkila RE. 1974. The generation of hydrogen peroxide, superoxide radical by 6-hydroxyl dopamine, dialuric acid and related agents. J Biol Chem 49: 2447-2452
3 Park BH, Rho HW, Park JW, Cho GG, Kim JS, Chung HT, Kim HR. 1995. Protective mechanism of glucose against alloxan-induced pancreatic $\beta$-cell damage. Biochem Biophys Res Commun 210: 1-6   DOI   ScienceOn
4 Grakvist K, Marklund S, Sehlin J, Taljedal IB. 1979. Superoxide dismutase, catalase and scavengers of hydroxyl radical protect against the toxic action of alloxan on pancreatic islet cells in vitro. Biochem J 182: 17-25   DOI
5 Fischer LJ, Hambruger SA. 1980. Inhibition of alloxan action in isolated pancreatic islets by superoxide dismutase, catalase and metal chelator. Diabetes 29: 213-216   DOI   ScienceOn
6 Grakvist K, MarkIund SL, Taljedal IB. 1981. Superoxide dismutase is a prophylatic against alloxan diabetes. Nature 294: 158-160   DOI   ScienceOn
7 Kim HR, Rho HW, Park BH, Park JW, Kim JS, Kim UH, Chung MY. 1994. Role of $Ca^{2+}$ in alloxan-induced pancreatic $\beta$-cell damage. Biochim Biophys Acta 1227: 87-91   DOI   ScienceOn
8 Steinmann GG, Esperester A, Joller P. 2001. Immunopharmacological in vitro effects of Eleutherococcus senticosus extracts. Arzneimittelforschung 51: 76-83
9 Glatthaar-Saalmuller B, Sacher F, Esperester A. 2001. Antiviral activity of an extract derived from roots of Eleutherococcus senticosus. Antiviral Res 50: 223-228   DOI   ScienceOn
10 Hibasami H, Fujikawa T, Takeda H, Nishibe S, Satoh T, Fujisawa T, Nakashima K. 2000. Induction of apoptosis by Acanthopanax senticosus HARMS and its component, sesamin in human stomach cancer KATO III cells. Oncol Rep 7: 1213-1216
11 Wagner H, Norr H, Winterhoff H. 1994. Plant adaptogens. Phytomedicine 1: 63-76   DOI
12 Rosengard BR, Cochrane DE. 1983. Complement-mediated cytolysis: A quick, simple method for determining levels of immunoglobulin E bound to mast cells. J Histochem 31: 441-444
13 Dunn JS, Sheehan HL, McLetchie NG. 1943. Necrosis of Langerhans produced experimentally. Lancet I: 484-487
14 Heikkila RE, Barden H, Cohen G. 1974. Prevention of alloxan-induced diabetes by ethanol administration. J Pharmacol Exp Ther 190: 501-506
15 Boquist L, Nelson L, Lorentzon R. 1983. Uptake of labeled in mouse organs and mitochondria in vivo and in vitro. Endocrinol 113: 943-948   DOI   ScienceOn
16 Ashcroft SJH, Hammonds P, Harrison DE. 1986. Insulin secretory responses of a clonal cell line of simian virus 40-transfected B cells. Diabetologia 29: 727-733   DOI   ScienceOn
17 Thaete LG. Grouch RK, Buse MG, Spicer SS. 1985. The protective role of copper-zinc superoxide dismutase against alloxan-induced diabetes: morphological aspects. Diabetologia 28: 677-682   DOI   ScienceOn
18 Gold G, Walker MD, Edwards DL, Grodsky GM. 1988. Biosynthetic regulation of endogenous hamster insulin and exogenous rat insulin II in transfected HIT cells. Diabetes 37: 1509-1514   DOI   ScienceOn
19 Malaisse WJ, Malaisse-Lagae F, Sener A, Pipeleers DG. Determinants of the selective toxicity of alloxan to the pancreatic B cell. Proc Natl Acad Sci USA 79: 927-930
20 Rerup CC. 1970. Drugs producing diabetes through damage of insulin secreting cells. Pharmacol Rev 22: 485-518
21 Heikkila RE, Cabbat FS. 1978. Protection against alloxaninduced diabetes in mice by the hydroxyl radical scavenger dimethylurea. Eur J Pharmacol 52: 57-60   DOI   ScienceOn
22 Heikkila RE, Cabbat FS. 1982. The prevention of alloxaninduced diabetes in mice by the iron-chelator detapac: suggestion of a role for iron in the cytotoxic process. Experientia 38: 378-379   DOI   ScienceOn
23 Okamoto H. 1985. Molecular basis of experimental diabetes: Degeneration, oncogenesis and regeneration of pancreatic $\beta$-cells of islet of Langerhans. Bio Assay 2: 15-21
24 Kim HR, Rho HW, Park JW, Kim JS, Kim UH, Jhee EC. 1991. Protection of alloxan-induced $\beta$-cell damage of rat pancreas by $Ca^{2+}$-antagonists. Kor J Biochem 23: 141-146
25 Yamamoto H, Uchigata Y, Okamoto H. 1981. DNA strand breaks in pancreatic islets by in vivo administration of alloxan or streptozotocin. Biochem Biophys Res Commun 103: 1014-1020   DOI   ScienceOn
26 Margaret MT, Irwin F. 2001. Methods of detection of vascular reactive species: nitric oxide, superoxide, hydrogen peroxide, and peroxynitrite. Circ Res 89: 224-236   DOI   ScienceOn
27 Miller FJ, Gutterman DD, Rios CD, Heistad DD, Davidson BL. 1998. Superoxide production in vascular smooth muscle contributes to oxidative stress and impaired relaxation in atherosclerosis. Circ Res 82: 1298-1305   DOI   ScienceOn
28 Bemofsky C, Swan M. 1973. An improved cycling assay for nicotinamide adenine dinucleotide. Anal Biochem 53: 452-458   DOI   ScienceOn
29 Takasu N, Asawa T, Komiya I, Nagasawa Y, Yamada T. 1991. Alloxan-induced DNA strand breaks in pancreatic islets: Evidence for $H_{2}O_{2}$ as an intermediate. J Biol Chem 266: 2112-2114
30 Cooperstein SJ, Watkins D. 1978. Effect of sulfuydryl binding reagents on islet tissue permeability: Protection and reversal by D-glucose and Phlorizin. J Pharmacol Exp Ther 204: 230-239
31 Gandy SE, Buse MG, Crouch PK. 1982. Protective role of superoxide dismutase against diabetogenic drugs. J Clin Invest 70: 650-658   DOI
32 Abdel-Rahman MS, Elrakhawy FI, Iskander FA. 1992. Protection of $\beta$-cells against the effect of alloxan. Toxicol Lett 63: 155-164   DOI   ScienceOn
33 Sui DY, Lu ZZ, Li SH, Cai Y. 1994. Hypoglycemic effect of saponin isolated from leaves of Acanthopanax senticosus (Rupr. et Maxin.) Harms. Zhongguo Zhong Yao Za Zhi 19: 683-685
34 Royall JA, Ischiropoulos H. 1993. Evaluation of 2',7'dichlorofluorescin and dihydrorhodamine 123 as fluorescent probes for intracellular $H_{2}O_{2}$ in cultured endothelial. Cells Arch Biochem Biophys 302: 348-355   DOI   ScienceOn