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http://dx.doi.org/10.3746/jfn.2004.9.3.265

Cytotoxic Effect of Fruit of Prunus mandshurica on Human Monocytic Leukemia Cells  

Chung, Ha-Sook (Department of Food and Nutrition, College of Natural Sciences, Duksung Women′s University)
Publication Information
Preventive Nutrition and Food Science / v.9, no.3, 2004 , pp. 265-269 More about this Journal
Abstract
Prunus mandshurica var. glabra Nakai (Rosaceae) is widely distributed in South Korea and bears a fruit with a bitter and astringent taste. An ethyl acetate-soluble extract of Prunus mandshurica was found to exhibit significant cytotoxicity against human leukemia cell lines. Bioassay-directed fractionation of this extract using an MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) cell proliferation assay as a monitor led to the isolation of the bioactive compounds. Two compounds, 1 and 2 were subsequently found to mediate cytotoxicity against U937, human monocytic leukemia cells. The 50% growth inhibitory concentrations ($IC_{50}$/) of compounds 1 and 2 on U937 were 40 and 62 $\mu\textrm{g}$/mL, respectively.
Keywords
Prunus mandshurica; flavonoid; cytotoxicity; human monocytic leukemia cells;
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