[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.3746/jfn.2003.8.4.301

Determination of Vitamin B₁₂ (Cyanocobalamin) in Fortified Foods by HPLC

Park, Youn-Ju (Division of Nutrition, Korea Food and Drug Administration)
Jang, Jae-Hee (Division of Nutrition, Korea Food and Drug Administration)
Park, Hye-Kyung (Division of Nutrition, Korea Food and Drug Administration)
Koo, Yong-Eui (Division of Nutrition, Korea Food and Drug Administration)
Hwang, In-Kyeong (Department Food and Nutrition, Seoul National University)
Kim, Dai-Byung (Division of Nutrition, Korea Food and Drug Administration)

Publication Information

Preventive Nutrition and Food Science / v.8, no.4, 2003 , pp. 301-305 More about this Journal

Abstract

This study was conducted to develop an HPLC method for determining vitamin B $_{12}$ in fortified foods which has typically been determined by microbiological assays according to AOAC and Korean Food Code approved methods. Vitamin B $_{12}$ (cyanocobalamin) was determined by reversed-phase HPLC with a triple column and UV/VIS dectector (550 nm) using the column switching technique after extraction with 5 mM potassium phosphate solution by sonication without a clean-up procedure. The recovery of spiked samples and limit of detection (LOD) by HPLC were 78.6 ∼107.5 % and 2 ppb ( $\mu\textrm{g}$ /kg), respectively. The LOD of the microbiological assay (MBA) was much lower than that of HPLC. The concentrations of vitamin B $_{12}$ analyzed in all tested samples (n=12) confirmed compliance with declared label claims. The range of recovery ratio by the HPLC method when compared to the microbiological assay was 76.2 ∼140.0 %. There was not significant difference between the HPLC and MBA methods (p < 0.01) with r=0.9791 and linear regression y=0.9923x-0.04. The HPLC method for determining vitamin B $_{12}$ using the column-switching technique appears to be suitable for determining vitamin B $_{12}$ concentrations above 1 $\mu\textrm{g}$ /100 g in fortified foods.ied foods.

Keywords

vitamin $B_{12}$ ; HPLC method; microbiological assay;

Citations & Related Records

Reference

1	/ [ Tom B ] / Nutritional Biochemistry
2	/ [ Eitenmiller RR;Landen WO Jr. ] / Vitamin Analysis for the Health and Food Sciences
3	/ [ Korea food & drug daministration ] / Korean food code
4	/ [ Korea food & drug administration ] / Korean food code(attend.)
5	Determination of cyanocobalmin, betamethason, and diclofenac sodium in pharmaceutical formulation, by high performance liquid chromatography / [ Gonzalez L;Yuln G;Volonte MG ] / J Pharm Biomed Anal DOI ScienceOn
6	Determination of cyanocobalamin in foods by high-performance liquid chromatography with visible detection after solid-phase extraction and membrance filtration for the precolumn separation of lipophilic species. / [ Iwase H;Ichiro O ] / J Chromatogr DOI ScienceOn
7	High performance liquid chromatographic separation of cobalamins / [ Frankel EP;Kitchens RL;Prough R ] / J Chromatogr DOI ScienceOn
8	Simultaneous determination of vitamin <TEX>$B_1$</TEX>, <TEX>B_2$</TEX>, <TEX>$B_6$</TEX>and <TEX>$B_12$</TEX> and C, nicotinamide and folic acid in capsule preparations by ion-pair reversed-phase high-performance liquid chromatography / [ Amin M;Reusch J ] / Analyst DOI ScienceOn
9	Official Methods Analysis, (16th ed.) / [ AOAC ] / Association of official analytical chemists
10	High performance liquid chromatography of water-soluble vitamins. II. Simultaneous determination of vitamins<TEX>$B_1$</TEX>, <TEX>B_2$</TEX>, <TEX>$B_6$</TEX>, and <TEX>$B_12$</TEX> in pharmaceutical preparations / [ Amin M;Reusch J ] / J Chromatogr DOI ScienceOn
11	Determination of cobalamins and cobinamides by microbore reversed-phase HPLC with spectrophotometric, ion-spray ionizaion MS and inductively coupled plasma MS detection. / [ Hubert C;Ryszard L ] / Analysis Chimica Acta
12	Determination of Vitamin <TEX>$B_12$</TEX> in foods using column-switching technique in <TEX>$\mu$</TEX>-HPLC / [ Park SJ;Kim HK;Hahm TS;Kim B ] / J Korean Soc Food Sci Nutr
13	/ [ The Korean Nutrition Society ] / RDA for Koreans
14	Ultramicrodetermination of cyanacobalamin in elemental diet by solid-phase extraction and high-performance liquid chromatography with visible detection / [ Iwase H ] / J Chromatogr DOI ScienceOn
15	Simultaneous liquid chromatographic determination water-soluble vitamins, caffeine, and preservative in oral liquid tonics / [ Yumie M;Masayoshi Y;Kazuhiro O;Shiro S;Toshio M;Hiroyuki N ] / J AOAC International
16	Simutaneous determination of cefoxitin, cefuroxine, cephalexin and cepaoridirine in plasma using HPLC and column-switching technique / [ Lee YJ;Lee HS ] / Chromatogrphia DOI
17	Quantitative analysis of retinoids in biological fluids by high performance liquid chromatography using column switching. I. determination of isotretinoin and tretinoin and their 4-oxo metabolites in plasma / [ Wyss R;Buchell F ] / J Chromatogr DOI ScienceOn
18	Simultaneous determination of parathion and metabolites in serum by HPLC column switching / [ Lee HS;Kim K;Kim JH;Do DS;Lee SK ] / Chromatographia DOI ScienceOn
19	Determination of pantothenic acid, biotin, and vitamin <TEX>$B_12$</TEX> in nutritional products, instrumental method for the analysis of vitamins / [ Thomas SH;Shyamala S;Rebecca A ] / J AOAC International
20	Analysis of an anti-inflammatory steroidal drug, difluprednate in aueous humor by combination of semi-micro HPLC and column switching method / [ Yasueda S;Kimura M;Ohtori A;Kakehi K ] / J Pharm Biomed Anal DOI ScienceOn
21	Column-switching high performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection / [ Nielsen SE;Dtrasted LO ] / J Chromatogr DOI ScienceOn
22	/ [ Choi HM ] / 21st Century Nutrition
23	Column-switching high performance liquid chromatographic assay for the determination of quercetin in human urine with ultraviolet absorbance detection / [ Nielsen SE;Dtrasted LO ] / J Chromatogr DOI ScienceOn
24	Analysis of an anti-inflammatory steroidal drug, difluprednate in aueous humor by combination of semi-micro HPLC and column switching method / [ Yasueda S;Kimura M;Ohtori A;Kakehi K ] / J Pharm Biomed Anal DOI ScienceOn
25	New ion-pair reagent for the high performance liquid chromatographic separation of B-group vitamins in pharmaceuticals / [ Woollard DC ] / J Chromatogr DOI ScienceOn

1	Analysis of Vitamin $B_{12}$ in the Korean Representative Foods and Dietary Intake Assessment for Koreans / [Choi, Youn-Ju;Kim, Ji-Yung;Lee, Haeng-Shin;Kim, Cho-Il;Hwang, In-Kyeong;Park, Hye-Kyung;Kim, Tae-Hwa;Oh, Chang-Hwan;] / Food Science and Biotechnology
2	Investigating Performance of Cotton and Lyocell Knit Treated with Microcapsules Containing Citrus unshiu Oil / [Lee, An Rye;Yi, Eunjou;] / Fibers and Polymers
3	Bioactive Properties of Citrus unshiu's Essential Oil for Its Application to Textiles / [Lee, An Rye;Yi, Eunjou;] / Fibers and Polymers
4	Preparation and Characterization of Melamine-formaldehyde Microcapsules Containing Citrus unshiu Essential Oil / [Lee, An Rye;Han, Chung Hun;Yi, Eunjou;] / Fibers and Polymers

12	An Rye Lee. (2013) Fibers and Polymers Investigating performance of cotton and lyocell knit treated with microcapsules containing Citrus unshiu oil / 14 (12) , 2088
8	An Rye Lee. (2013) Fibers and Polymers Bioactive properties of Citrus unshiu’s essential oil for its application to textiles / 14 (8) , 1295
1	An Rye Lee. (2014) Fibers and Polymers Preparation and characterization of melamine-formaldehyde microcapsules containing Citrus unshiu essential oil / 15 (1) , 35
6	(2018) Molecules A Review of the Extraction and Determination Methods of Thirteen Essential Vitamins to the Human Body: An Update from 2010 / 23 (6) , 1484

KSCI

Determination of Vitamin B12 (Cyanocobalamin) in Fortified Foods by HPLC

Determination of Vitamin B₁₂ (Cyanocobalamin) in Fortified Foods by HPLC