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http://dx.doi.org/10.3746/jfn.2003.8.2.196

Inhibition of Listeria monocytogenes in Vacuum or Modified Atmosphere-Packed Ground Beef by Lactococcal Bacteriocins  

Park, Hye-Jung (Division of Life Sciences, Kyungnam University)
Lee, Na-Kyoung (Division of Life Sciences, Kyungnam University)
Kim, Kee-Tae (Proco Biotech Co.)
Ha, Jung-Uk (Division of Life Sciences, Kyungnam University)
Lee, Dong-Sun (Division of Life Sciences, Kyungnam University)
Paik, Hyun-Dong (Division of Life Sciences, Kyungnam University)
Publication Information
Preventive Nutrition and Food Science / v.8, no.2, 2003 , pp. 196-199 More about this Journal
Abstract
We investigated the antagonistic effects of two lactococcal bacteriocins, nisin or lacticin NK24, on the growth and the survival of Listeria monocytogenes in vacuum or modified atmosphere-packaged ground beef, Ground beef was inoculated with approximately 3 log colony-forming units (CFU) of L. monocytogenes ATCC 15313 culture per gram of ground beef. Inoculated samples were blended with/without 100 AU/g nisin or lacticin NK24, and subsequently vacuum or modified atmosphere packed at 4$^{\circ}C$. Listeria in the bacteriocin-treated and control samples was subsequently isolated from both vacuum and modified atmosphere packs and enumerated as CFU on Listeria Isolation Agar medium. Microbial counts in ground beef treated with bacteriocin declined steadily, while those of non-treated beef samples increased steadily. The results obtained demonstrate that nisin inhibits the growth of L. monocytogenes more effectively than lacticin NK24 at 100 AU/g. The use of lactococcal bacteriocins, such as nisin or lacticin NK24, in vacuum or modified atmosphere packaging offers a promising approach for eliminating or reducing the risk of L monocytogenes contamination in ground beef.
Keywords
ground beef; bacteriocin; nisin; lacticin NK24; Listeria monocytogenes; modified atmosphere packaging; vacuum packaging;
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