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http://dx.doi.org/10.3746/jfn.2003.8.2.124

Suppressive Effect of Chlorella Methanol Extract on Oxidative Stress and NFkB Activation in RAW 264.7 Macrophages  

Park, Ji-Young (School of Food Life Science and Biohealth Product Research Center, Inje University)
Lee, Hyo-Sun (School of Food Life Science and Biohealth Product Research Center, Inje University)
Song, Young-Sun (School of Food Life Science and Biohealth Product Research Center, Inje University)
Publication Information
Preventive Nutrition and Food Science / v.8, no.2, 2003 , pp. 124-129 More about this Journal
Abstract
This study was designed to investigate whether a methanol extract of chlorella can suppress oxidative stress and nuclear factor kB (NFkB) activation in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cells. Treatment of RAW 264.7 cells with chlorella methanol extract (25, 50, and 100 $\mu$g/mL) significantly reduced LPS-stimulated nitric oxide production in a dose-dependent manner. Treatments with chlorella methanol extract at all concentrations also reduced thiobarbituric acid-reactive substances accumulation and enhanced glutathione level at 50 and 100 $\mu$g/mL levels. The specific DNA binding activities of NFkB on nuclear extracts in cells treated with 50 $\mu$g/mL and 100 $\mu$g/mL chlorella methanol extracts were significantly suppressed. These results suggest that chlorella methanol extract has mild antioxidative activity and the ability to suppress intracellular oxidative stress and NFkB activation.
Keywords
chlorella methanol extract; oxidative stress; NFkB; NO; macrophages;
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