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http://dx.doi.org/10.3746/jfn.2002.7.4.421

Purification and Characterization of an Intracellular Inulinase from Bacillus sphaericus 188-1  

Kim, Jae-Ho (Department of Genetic Engineering and Bio-Med. RRC, Paichai University)
Kwak, Yoon-Jin (Department of Genetic Engineering and Bio-Med. RRC, Paichai University)
Lee, Jong-Tae (Central Research Institute, Korea Tabacco and Ginseng Corporation)
Park, Shin-Yang (Korea Food Research Institute)
Lee, Jong-Soo (Department of Genetic Engineering and Bio-Med. RRC, Paichai University)
Publication Information
Preventive Nutrition and Food Science / v.7, no.4, 2002 , pp. 421-426 More about this Journal
Abstract
In order to obtain basal data for industrial application of inulinase from Bacillus sphaeicus 188-1, its intracellular inulinase was purified by ammonium sulfate fractionation and column chromatography on DEAE-Sephadex A-50 and Sephadex G-100. The enzyme was homogeneous as judged by SDS-polyacrylamide gel electrophoresis, with an apparent molecular weight of 29 kDa. Inulinase activity was optimal at pH 6.5 and 4$0^{\circ}C$. The enzyme activity was significantly inhibited by Cu$^{2＋}$, Cd$^{2＋}$ and Hg$^{2＋}$. The inulinase exhibited an apparent Km value of 0.014% for inulin.
Keywords
Bacillus sphaericus 188-1; intracellular inulinase; characterization;
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