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http://dx.doi.org/10.3746/jfn.2002.7.4.417

Fibrinolytic Enzyme Production by Bacillus subtilis KH-4 Isolated from Deonjang  

Kim, J.M. (Department of Food and Nutrition, College of Health Science, Korea University)
Suh, H.J. (Department of Food and Nutrition, College of Health Science, Korea University)
Ahn, S.W. (Neurotide LTD)
Kim, M.S. (Biotech. Division, Pacific Corporation)
Oh, S.H. (Department of Food Engineering, Ansan College of Technology)
Publication Information
Preventive Nutrition and Food Science / v.7, no.4, 2002 , pp. 417-420 More about this Journal
Abstract
A strong fibrin-specific fibrinolytic enzyme was produced from Bacillus subtilis KH-4 isolated from Deonjang, a Korean fermented soybean paste similar to Japanese miso. The addition of glucose as a carbon source resulted in the highest levels of caseinolytic and fibrinolytic activities. Likewise, the addition of yeast extract as the nitrogen source resulted in the highest caseinolytic and fibrinolytic activities (3473.2 unit and 47.4 munit, respectively), It was observed that out of all metal ion sources only calcium (chloride) enhanced caseinolytic and fibrinolytic activities, with increases of 4949.3 unit and 58.2 unit/mg, respectively. The optimal temperature for the production of the enzyme was found to be 4$0^{\circ}C$ in the optimal medium (glucose 20 g, yeast extract 5 g, CaCl$_2$l g, and NaCl 2 g). The maximum fibrinolytic activity was observed at the late stationary phase. B. subtilis KH-4 produced a fibrinolytic enzyme at 4$0^{\circ}C$, after 30 h growth, which increased up to 54 h and then remained constant. These results suggest that Deonjang has potential as a source of physiologically active anti-thromotic enzymes.
Keywords
fibrinolytic enzyme; caseinolytic activity; Bacillus subtilis;
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1 Jang SA, Kim MH, Lee MS, Lee MJ, Jhee OH, Oh TK, Sohn CB. 1999. Isolation and identification of fibrinolytic enzyme producing strain from shrimp Jeot-gal, a tiny salted shrimps, and medium optimization for enzyme production. Korean J Food Sci Technol 31: 1648-1653
2 Lijnen HR, van Hoef B, de Cock F, Okada K, Ueshima S, Matsuo O, Collen D. 1991. On the mechanism of fibrin- specific plasminogen activation by staphylokinase. J Biol Chem 266: 11826-11832
3 Medved LV, Solovjov DA, Ingham KC. 1996. Domain structure, stability and interactions in streptokinase. Eur J Biochem 239: 333-339   DOI   ScienceOn
4 Arah K, Mimuro J, Madoiwa S, Matsuda M, Sako T, Sakata Y. 1995. Effect of staphylokinase concentration of plasminogen activation. Biochim Biophy Acta 1245: 69-75   DOI   ScienceOn
5 Sumi H, Hamada H, Tsushima H, Mihara H, Muraki H. 1987. A nevel fibrinolytic enzyme in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet. Experientia 43: 1110-1111   DOI   ScienceOn
6 Nakamura T, Yamagata Y, Ichishima E. 1992. Nucleotide sequence of the subtilis NAT gene, arpN, of Bacillus subtilis (natto). Biosci Biotechnol Biochem 56: 1869-1871   DOI   ScienceOn
7 Sumi H, Hamada H, Nakanishi K, Hiratani H. 1990. Enhancement of the fibrinolytic activity in plasma by oral administration of NK. Acta Haematol 84: 139-143   DOI
8 El-Aassar SA. 1995. Production and properties of fibrinolytic enzyme in solid state culture of cultures of Fusarium pallidoroseum. Biotechnol Lett 17: 943-948   DOI
9 Reed GL, Lin LF, Parhami-Seren B, Kussie P. 1995. Identification of plasminogen binding region in streptokinase that is necessary for the creation of functional streptokinase- plasminogen activator complex. Biochemistry 34: 10266-10271   DOI   ScienceOn
10 Harerkate F, Traas DW. 1974. Dose response curves in the fibrin plate assay to determined the fibrinolytic activity of proteases. Thromb Haemostasis 32: 357-365
11 Bascaran V, Hardisson C, Brana AF. 1990. Regulation of extra-cellular protease production in Streptomyces clavultigenas. Appl Microbiol Biotechnol 39: 208-213
12 Sumi H, Maruyama M, Yoneta T, Mihara H. 1983. Activation of plasma fibrinolysis after intrarectal administration of high molecular weight urokinase and its derivative. Acta Haematol 70: 289-295   DOI
13 Longinova LG, Usaite IA, Seregina LM. 1980. Isolation of unpigmented producer of proteolytic enzymes from Thermoactinomyces vulgaris. Appl Biochem Microbiol 16: 24-30
14 Mohamedin AH. 1999. Isolation, identification and some cultural conditions of a protease-producing thermophilic Streptomyces strain grown on chicken feather as a substrate. Int Biodeter Biodegr 43: 13-21   DOI   ScienceOn
15 Chitte RR, Dey S. 2002. Production of a fibrinolytic enzyme by thermophilic Streptomyces species. World J Microb Biotechnol 18: 289-294   DOI
16 Heo S, Lee SK, Joo HK. 1998. Isolation and identification of fibrinolytic bacteria from Korean traditional Chungkoojang. Kor J Agr Chem Biotechnol 41: 119-124
17 Wun TC, Schleuning WD, Reich E. 1982. Isolation and characterization of urokinase from human plasmin. J Biol Chem 257: 3276-3283
18 Pennica D, Holmes WE, Kohr WJ, Harkins RN, Vehar GA, Ward CA, Bennett WF, Yelverton E, Seeburg PH, Heyneker HL, Goeddel DV, Collen D. 1983. Cloning and expression of human tissue-type plasminogen activator cDNA in E. coli. Nature 301: 214-221   DOI   ScienceOn
19 Kim WK, Choi KH, Kim YT, Park HH, Choi JY, Lee YS, Oh HI, Kwon IB, Lee SY. 1996. Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. Strain CK 11-4 screened from Chung-Kook- Jang. Appl Environ Microbiol 62: 2482-2488
20 Fayek KI, El-Sayed ST. 1980. Fibrinolytic activity of an enzyme produced by Bacillus subtilis. Z Ernaehrwiss 19: 21-23
21 Kim HK, Kim GT, Kim DK, Choi WA, Park SH, Jeong YK, Kong IS. 1997. Purification and characterization of a novel fibrinolytic enzyme produced from Bacillus sp. KA 38 orginated from fermented fish. J Ferment Bioeng 84: 307-312   DOI   ScienceOn