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http://dx.doi.org/10.3746/jfn.2002.7.2.123

Inhibitory Effects of Ninety Nine Korean Plants on Human Immunodeficiency Virus Type 1 Pretense Activity  

Park, Jong-Cheol (Department of Oriental Medicine Resources)
Hur, Jong-Moon (Department of Oriental Medicine Resources)
Park, Ju-Gwon (Department of Oriental Medicine Resources)
Hyun, Kyu-Hawn (Department of Resources Plants, Sunchon National University)
Lee, Kab-Yeon (Sobu Forest Experiment Station, Korea Forest Genetics Research Institute)
Hirotsugu Miyashiro (Institute of Natural Medicine, Toyama Medical and Pharmaceutical University)
Masao Hattori (Institute of Natural Medicine, Toyama Medical and Pharmaceutical University)
Publication Information
Preventive Nutrition and Food Science / v.7, no.2, 2002 , pp. 123-127 More about this Journal
Abstract
Ninety nine extracts from Korean plants were screened for their inhibitory activities on human immunodeficiency virus (HIV) type 1 pretense by an HPLC method. The pretense inhibitory activities were determined by incubating the extracts in reaction mixtures containing pretense and substrate (His-Lys-Ala-Arg-Val-Leu-(p-NO$_2$- Phe)-Glu-Ala-Nle-Ser-NH$_2$) to perform proteolytic cleavage reactions. Of the extracts tested, the water extracts of Viburnum awabuki (stem and leaves) and Distylium racemosum (leaves) had the highest pretense inhibitory activities at a concentration of 100ug/mL. Activity-guided fractionation, revealed that the n-butanol fraction of the V. awabuki extract and the ethyl acetate fraction from the D. racemosum extract had the greatest inhibitory activity on HIV-1 pretense.
Keywords
HIV-1 protease; protease inhibitor; Korea plants;
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