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http://dx.doi.org/10.17495/easdl.2016.10.26.5.389

Hepatotoxicity Reducing Effect of Ethanol Extracts from Fermented Youngia denticulata Houtt Kitamura in Ethanol-treated Rats  

Kim, Mee-Jung (Dept. of Food Science and Technology, Chung-ang University)
Lee, Hyun-Koo (LHK Fermentation Lab.)
Publication Information
Journal of the East Asian Society of Dietary Life / v.26, no.5, 2016 , pp. 389-399 More about this Journal
Abstract
This study was performed to investigate the hepatotoxicity reducing effect of ethanol extracts from Youngia denticulata Houtt Kitamura (YD) fermented by Aspergillus oryzae, which is well known as a Korean traditional meju microbe. Experimental rats were divided into 4 diets groups (six rats/group) (NC: normal control group, EC: ethanol supplemented control group, ENY: ethanol extracts 0.5% supplemented diets of the non-fermented YD group, EFY: ethanol extracts 0.5% supplemented diets of the fermented YD group). After feeding them for four weeks, the activities GOT and GPT in serum, contents of neutral lipid, total cholesterol, LPO and GSH, and activities of XO (O type) enzyme, SOD and GST in liver tissue were observed. Compared to the NC group, the GOT and GPT activities in the EC group were markedly increased by 87.91% and 57.02%, and the contents of neutral lipid, total cholesterol and LPO were increased by 77.51%, 18.56% and 193.86%, respectively. On the other hand, the GSH content was decreased by 29.52%. The activities of SOD and GST which are anti-oxidation-related enzymes, were decreased by 42.78% and 0.94%, respectively, compared to the NC group, but the XO (O type) enzyme activity was significantly increased by 70.83%. These results confirmed that the liver tissue and anti-oxidation system of the EC group had been damaged. Compared to the EC group, the GOT and GPT activities of the ENY and EFY groups were significantly decreased by 24.73~41.99% and 22.91~30.11%, respectively. The contents of neutral lipid, total cholesterol and LPO were decreased by 16.65~33.33%, 22.44~23.11% and 63.34~66.13%, respectively, but the GSH content was significantly increased by 24.61~35.60%. The GST activity was not significantly affected, but the SOD activity was significantly increased by 214.94~261.19%. The XO (O type) enzyme activity was significantly decreased by 40.34~51.20%. Compared to the ENY group, the GOT and GPT activities of the EFY group were significantly decreased by 22.93% and 9.34%, respectively. The plasma contents of neutral lipid, total cholesterol and LPO were decreased by 20.01%, 0.86% and 7.59%, respectively. The SOD activity was significantly increased by 21.52%, and the XO (O type) enzyme activity was decreased by 18.19%. Given decreasde TG, total cholesterol, LPO contents of liver tissue, GOT, GPT and XO activity, and increased SOD activity, these study results that ethanol extracts from fermented Youngia denticulata Houtt Kitamura may be effective in reducing hepatotoxicity.
Keywords
Youngia denticulata Houtt Kitamura; fermentation; Aspergillus oryzae; hepatotoxicity; anti-oxidation-related enzymes activity;
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1 Choi JS, Young HS, Kim BW (1991) Hypoglycemic and hypolipidemic effects of Ireris dentata in diabetes rats. Arch Pharm Res 13: 269-274.
2 Son JC, Kim SH, Lee SI, Lee YK, Kim SD (2012) Effect of ethanol extracts of Youngia denticulata and Youngia sonchifolia on the serum and hepatic lipids and activities of ethanol metabolizing enzymes in acute ethanol-treated rats. J Korean Soc Food Sci Nutr 41(2): 187-204.
3 Song JT, Jeong HB, Kim BW, So HS (1989) Plant Large Handbook (resources side). Il-Dong Publishing Co, Incheon, Korea. pp 334-336.
4 Stirpe F, Della Corte E (1969) The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase(type D) to oxidase (type O). J Biol Chem 244(14): 3855-3863.
5 Wang RS, Nakajima T, Honma T (1999) Different change patterns of the isozymes of cytochrome P450 and glutathione S-transferase in chemically induced liver damage in rat. Ind Health 37(4): 440-448.   DOI
6 Williams R (2006) Global challenges in liver disease. Hepatology 44: 521-526.   DOI
7 Yamamoto Y, Yasuoka A, Tachikawa N, Reruya K, Genka, Yamaguchi M, Yasuoka C, Kikuchi Y, Aoki M, Okas S (1999) Mitigation of hepatocellular injury caused by HAART with glycyrrhizin compound in patients co-infected wth HIV and HCV. Jpn J Infect Dis 52: 248-249.
8 Zeng T, Guo FF, Zhang CL, Zhao S, Dou DD, Gao XC, Xie KG (2008) The anti-fatty liver dffects of garlic oil on acute ethanol-exposed mice. Chem Biol Interact 176: 234-242.   DOI
9 Choi JS, Yoon TJ, Kang KR, Lee KH, Kim WH, Suh YH, Song J, Jung MH (2006) Glycoprotein isolated from Acanthopanax senticosus protects against hepatotoxicity induced by acute and chronic alcohol treatment. Biol Pharm Bull 29: 306-314.   DOI
10 Cunningham CC, Bailey SM (2001) Ethanol consumption and liver mitochondria function. Biol Signals Recept 10: 271-282.   DOI
11 Kim YH (2004) Anti-HBV viral multiplication by the extracts of Lactuca indica. J Natual Sciences, Suwon University 3(2): pp 237-244.
12 Kim JG (1989) Illustrated Natural Drugs Encyclopedia. Published by Nam-San Dang. pp 58-60.
13 Kim JG (1992) Illustrated Natural Drugs Encyclopedia(Color edition). Published by Nam-San Dang, Seoul, Korea, Vol.1 pp 42-47.
14 Kim SH (1995) Inhibitory effects of Ixeris dentata on the mutagenicity of aflatoxin B1, N-methyl-N'-nitro-N-nitrosoguanidine and the growth of MG-63 human osteosarcoma cells. J Korean Soc of Food Sci Nutr 24(2): 305-320.
15 Kim YJ, You YH, Jun WJ (2012) Hepatoprotective activity of fermented Curcuma longa L. on galactosamine-intoxicated rats. J Korean Soc Food Sci Nutr 41(6): 790-795.   DOI
16 Korean National Statistical Office (2016) Statistics Index of Alcohol Delivery Amount in 2014. http://www.index.go.kr/main/eachDtlpageDetail.do?idx-cd=2824 Accessed 2016.
17 Kundu TK, Hille F, Velayutham M, Zweier JL (2007) Characterization of superoxide production from aldehyde oxidase:An important source of oxidants in biological tissues. Arch Biochem Biophys 460: 113-121.   DOI
18 Lands WEM, Pawlosky RJ, Salem N (1999) Alcoholism, antioxidant status and essential fatty acids. In Antioxidant Status, Diet, Nutrition and Health. Papas AM, ed. CRC Press, Boca Raton, FL, USA. pp 299-344.
19 Kim MJ, Lee E, Cha BC, Choi MY, Rhim TJ, Park HJ (1997) Serum cholesterol lowering effect of triterpene acetate obtained from Lactuca indica. Korean J Pharmacogn 28: 21-25.
20 Satoh K (1978) Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Czlinica Chimica Acta 90(1): 37-43.   DOI
21 De Ritis R, Giusti G, Prccinino F, Cacciatore L (1965) Biochemical laboratory tests in viral hepatitis and other hepatic diseases: Evaluation and follow-up. Bull World Health Organ 32: 59-72.
22 Das SK, Nayak P, Vasudevan DM (2005) Consequences of ethanol consumption. J Indian Soc Toxicol 1: 1-10.
23 Das SK, Vasudevan DM (2007) Alcohol-induced oxidative stress. Life Sci 81: 177-187.   DOI
24 Demori I, Voci A, Fugassa E, Burlando B (2006) Combined effects of high-fat diet and ethanol induce oxidative stress in rat liver. Alcohol 40: 185-191.   DOI
25 Ellman GL (1959) Tissue sulfhydryl group. Arch Biochem Biophys 82(1): 70-77.   DOI
26 Fang JL, Vaca CE (1997) Detection of DNA adducts of acetaldehyde in peripheral white blood cells of alcohol abusers. Carcinogenesis 18: 627-632.   DOI
27 Folch J, Less M. (1957) A simple method for the isolation and purification of total lipids from animal tissues. J Biolchem 226(1): 497-509.
28 Forrnan HJ, Fridovich I (1973) Superoxide dismutase: A comparison of rate constants. Arch Biochem Biophys 158: 396-402.   DOI
29 Ahn HY, Park KR, Kim YR, Yoon KH, Kim JW, Cho YS (2013) Effects of Monascus-fermented Angelica gigas Nakai on the contents of serum lipid and tissue lipid peroxidation in alcohol feeding rats. J of Life Science 23(11): 1371-1380.   DOI
30 Lee J, Jeong JY, Cho YS, Park SK, Kim K, Kim MJ, Lee MK (2010) Effect of young Phragmites communis leaves powder on lipid metabolism and erythrocyte antioxidant enzyme activities in highfat diet fed mice. J Korean Soc Food Sci Nutr 39(5): 677-683.   DOI
31 Lieber CS (1992) Medical and Nutritional Complications of Alcoholism: Mechanisms and Management. Plenum Press, New York, NY, USA, pp 515-530.
32 Arteel G, Marsano L, Mendez C, Bentley F, McClain C (2003) Advances in alcoholic liver disease. Best Pract Res Clin Gastroenterol 17(1): 625-647.   DOI
33 Lee SI, Lee YK, Kim SD, Lee IA, Choi JK, Suh JW (2013) Effects of hot-water extract of mulberry leaf tea fermented by Monascus pilosus on body weight and hepatic antitoxidant enzyme activities in mouse fed a normal diet. J Korea Academia-Industrial Cooperation Society 14(11): 5646-5657.   DOI
34 Lee SI, Lee YK, Kim SD, Cheng JH, Yang SH, Suh YJ (2014) Xanthine and aldehyde oxidase inhibitory activities, and antihyperuricemic effects of fermented Smilax china L. leaf extracts and fractions. J Appl Biol Chem 57(1): 53-59.   DOI
35 Lieber CS (2004) Alcoholic fatty liver: Its pathogenesis and mechanism of progression to inflammation and fibrosis. Alcohol 34: 9-19.   DOI
36 Lieber CS (1997) Ethanol metabolism, cirrhosis and alcoholism. Clin Chim Acta 257: 59-84.   DOI
37 Lieber CS (1985) Alcohol and the liver: Metabolism of ethanol, metabolism effects and pathogenesis of injury. Acta Med Scand Suppl 703: 11-55.
38 Lin RC, Smith RS, Lumeng L (1988) Detection of a proteinacetaldehyde adduct in the liverof rats fed alcohol chronically. J Clin Invest 81: 615-619.   DOI
39 Lee K (1991) Developmental aspects of experimental pulmonary oxygen toxicity. Free Rad Biol 11: 463-494.   DOI
40 Machida M, Tamada O, Gomi K (2008) Genomics of Aspergillus oryzae: learning from the history if koji mold and exploration of its future. DNA Res 15: 173-183.   DOI
41 Bompart GJ, Prevot DS, Bascands JL (1990) Rapid automated analysis of glutathione reductase, peroxidase and S-transferase activity: Application to cisplatin-induced toxicity. Clin Bio Ilem 23: 501-510.   DOI
42 Bae SJ, Kim NH, Ha BJ, Jung BM, Roh SB (1997) Effects of Godulbaegi (Ixeris sonchifolia H.) diet on enzyme activities of CCl4 induced hepatotoxicity in rats. J Korean Nutr 30(1): 19-24.
43 Bae SJ, Kim NH, Ha BJ, Jung BM, Roh SB (1997) Effects of Godulbaegi leaf extracts on CCl4 induced hepatotoxicity in rats. J Korean Soc Food Sci Nutr 26: 137-143.
44 Baek GY (2000) Effect of hot water extracts from Plantago asiatica var. densiuscula Pilg ilg, Ixeris dentata Nakai and Morus alba L. on detoxication of liver in $CCL_4$ treated rats. Ph D Dissertation Daegu Catholic University, Daegu. pp 30-45.
45 Cederbaum AI (1991) Micrisimal generation of reactive oxygenspecies and their possible role in alcohol hepatotoxicity. Alcohol Suppl 1: 291-296.
46 Chirtchai P, Chaiyavat C, Sunee T, Somsak V, Wonnop PS (2011) 8-Hydroxygenistein formation of soybean fermented with Aspergillus oryzae BCC 3088. Afr J Agric Res 6: 785-789.
47 Ahn HY (2014) Effect of fermeneted Angelica gigas Nakai on hepatotoxicity and oxidative stress in the rat. Ph D Dissertation, Dong A University, Pusan. pp 170-186.
48 Choi JS, Chung HY, Young HS (1990) Aprelinary study on hypocholesterolemia and hypoglycemic activities of some medical plants. Kor T Pharmacogn 21: 153-156.
49 Habig WH, Pabist M, Jakoby WB (1974) Glutathione-S-transferase: The frrst enzymatic steps in mercapturic acid forrnation. J Biol Chem 249: 7130-7135.
50 Mantle D, Preedy VR (1999) Free radicals as mediators of alcohol toxicity. Adverse Drug React Toxicol Rev 18: 235-252.
51 Niemala O, Klajner F, Orrego H, Vidins E, Blendis L, Israel Y (1987) Antibodies against acetaldehyde-modified protein epitopes in human alcoholics. Hepatology 7: 1210-1214.   DOI
52 Marui J, Tada S, Fukuoka M, Wagu T, Shiraishi Y, Kitamoto N, Sugomoto T, Hattori R, Suzuki S, Kusumoto K (2013) Reduction of the degradation activity of umami-enhancing purinic ribonucleotide supplement in miso by the targeted suppression of acid phosphatases in the Aspergillus oryzae starter culture. Int J Food Microbiol 166: 238-243.   DOI
53 Martin JP, Dailey JM, Sugarmanand E (1987) Negative and positive assays of superoxide dismutase based on hematoxylin autoxidant. Arch Biochem Biophys 255: 329-336.   DOI
54 Mello T, Ceni E, Surrenti C, Galli A (2008) Alcohol induced hepatic fibrosis: Role of acetaldehyde. Mol Aspects Med 29: 17-21.   DOI
55 Ohkawa H, Ohishi N, Yagi K (1979) Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95(2): 351-358.   DOI
56 Park JK, Park CM, Kim JJ, Song YS (2008) Hepatoprotective activity of dandelion (Taraxacum officinale) water extract against D-galactosamine-induced hepatitis in rats. J Korean Soc Nutr 37: 177-183.   DOI
57 Piras G, Makino M, Baba M (1997) Sho-saiko-to, a traditional kampo medicine, enhances the anti-HIV-1 activity of lamivudine (3TC) in vitro. Micorbio Immunol 41(10): 835-839.   DOI
58 Reitman S, Frankel S (1957) A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am J Clin Pathol 28: 56-63.   DOI
59 Han EK, Jin YX, Yoo YS, Jung EJ, Lee JY, Chung CK (2009) Effect of Artemisia capillaris and Paecilomyces japonica on the reduction of hepatotoxicity and lipid metabolism induced by ethanol. J Korean Sic Food Sci Nutr 38(1): 1016-1023.   DOI
60 Ham YK, Kim SW (2004) Protective effects of plant extract on the hepatocytes of rat treated with carbon tetrachloride. J Korean Soc Food Sci Nutr 33(3): 1246-1251.   DOI
61 Hashim MS, Lincy S, Remya V, Teena M, Anila L (2005) Effect of polyphenolic compounds from Coriandrum sativum on $H_2O_2$-induced oxidative stree in human lympocytes. Food Chem 92(4): 653-660.   DOI
62 Hilton JW, Hodson PV, Slinger SJ (1980) The requirement and toxicity of selenium in rainbow trout (Salmo gairdneri). J Nutr 110: 2527-2535.   DOI
63 Hong KJ, Lee CH, Kim SW (2004) Aspergillus oryzae CB-107 fermentation improves nutritional quality of food soybeans and feed soybean meals. J Med Food 7: 403-435.
64 Jeon HJ, Yu S, Kim SH, Park SK, Choi HD, Kim KY, Lee SY, Chun SS, Ahn SC (2014) Antiobesty effect of Citrus peel extract fermented with Aspergillus oryzae. J Life Sci 24: 827-836.   DOI
65 Jin JI (1985) Encyclopedia of Chinese Medical Science III. Chinese Dictionary Association. Published by Song-Ak. pp130.
66 Kang YH, Kim K, Kim TW, Yang CS, Choe M (2015) Evaluation of the anti-obesity activity of Platycodon grandiflorum root and Curcuma Ionga root fermented with Aspergillus oryzae. Korean J Food Sci Technol 47(1): 111-118.   DOI
67 Khanal T, Choi JH, Hwang YP, Chung YC, Jeong HG (2009) Saponins isolated from the root of Platycodon grandiflorum protect against acute ethanol-induced hepatotoxicity in mice. Food Chem Toxicol 47: 530-535.   DOI
68 Ryle PR, Chakraborty J, Thomson AD (1986) The role of the hepatocellular redox state in the hepatic triglyceride accumulation following acute ethanol administration. Biochem Phamacol 35: 3159-3164.   DOI