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http://dx.doi.org/10.17495/easdl.2015.2.25.1.87

Effect of Grape Skin Extract on Apoptosis in MDA-MB-231 Human Breast Cancer Cells  

Park, Min-A (Dept. of Food & Nutrition, College of Natural Science, DukSung Women's University)
Kang, Keum-Jee (Dept. of Food & Nutrition, College of Natural Science, DukSung Women's University)
Publication Information
Journal of the East Asian Society of Dietary Life / v.25, no.1, 2015 , pp. 87-98 More about this Journal
Abstract
We investigated the apoptotic effects of grape skin extracts (GSE) and related gene expressions in human breast cancer MDA-MB-231 cells cultured in the presence of 0, 0.5, 1 and 1.5 mg/mL of GSE for 72 hours. MTT assay, trypan blue and nuclei staining showed lower cellular mitochondrial activities and increased cell deaths with a higher concentration of GSE (p<0.05). Increased cell number with fragmentated DNA of sub-G1 phase was calculated as a measure of apoptotic cell death by FACS analysis (p<0.05). In particular, apoptotic cell death caused markedly increased in the 1 and 1.5 mg/mL of GSE groups, as revealed by flow cytometry (Annexin V-FITC). RT-PCR analysis was performed on apoptotic and preapoptotic genes. Expression of the apoptosis suppressor gene bcl-2 significantly decreased, proapoptotic gene bax was significantly increased and procaspase-3 showing the presence of caspase-3 significantly decreased (p<0.05). Furthermore, bcl-2/bax ratio which is considered to be an important indicator of apoptosis, significantly decreased in a concentration-dependent manner (p<0.05). These results indicated that GSE induces apoptosis in MDA-MB-231 human breast cancer cells.
Keywords
Grape skin; MDA-MB-231 cell; apoptosis; Bcl-2; Bax; caspase-3;
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