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http://dx.doi.org/10.5187/JAST.2004.46.4.547

Chromosomal Localization and Distribution of the Telomeric DNA in Cattle and Pigs  

Sohn, S.H. (Department of Animal Science and Biotechnology & RAIRC, Jinju National University)
Multani, A.S. (The University of Texas, MD Anderson Cancer Center)
Pathak, S. (The University of Texas, MD Anderson Cancer Center)
Cho, E.J. (Department of Animal Science and Biotechnology & RAIRC, Jinju National University)
Ha, H.B. (Department of Animal Science and Biotechnology & RAIRC, Jinju National University)
Publication Information
Journal of Animal Science and Technology / v.46, no.4, 2004 , pp. 547-554 More about this Journal
Abstract
Telomeres are nucleoprotein structures at the ends of chromosomes consisting of tandem repeat sequences of . (TTAGGG)n. Telomeres serve as guardians of the genome, protect individual chromosomes within the nucleus, and help in meiotic pairing of homologous chromosomes. To investigate the telomere distributions of cattle and pig chromosomes, fluorescence in situ hybridization(FISH) was carried out on metaphase spreads of in vitro fibroblast cultures from Holstein and Landrace using a human telomeric DNA repeat probe. Results indicate that the distinct double spots on both ends of chromosomes of cattle and pigs were observed. In cattle, there was a random variation in the intensity of telomere signals among chromosomes. In pigs, an interstitial telomeric signal was observed on the chromosome 6q1 of all the cells examined. According to quantitative fluorescence in situ hybridization(Q-FISH) analysis, some chromosomes had consistently much more telorneres at one end of chromosomes. In general, both species had consistently much more telomeres at q-end than p-end on most of chromosomes. The relative amount of telomeres on bovine chromosomes was higher than that on pig chromosomes. In additions, Y chromosome had the highest relative amount of telorneres in cattle and pigs.
Keywords
Telomeres; Q-FISH; Chromosomes; Cattle; Pigs;
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1 Slijepcevic, P. 2001. Telomere length measurement by Q-FISH. Cell Sci. 23:17-22.
2 Sohn, S. H., Multani, A. S., Gugnani, P. K. and Pathak, S. 2002. Telomere erosion-induced mitotic catastrophe in continuously grown Chinese hamster Don cells. Exptl. Cell Res. 279(2):271-276.
3 Ahmed, A. and Tollefsbol, T. 2001. Telomeres and telomerase: basic science implications for aging. J. Am. Geriatr. Soc. 49(8):1105-1109.
4 Betts, D. H., Bordingnon, V., Hill, J. R., Winger, Q., Westhusin, M. E., Smith, L. C. and King, W. A. 2001. Reprogramming of telomerase activity and rebuilding of telomere length in cloned cattle. Proc. Natl. Sci. USA 98:1077-1082.   DOI   ScienceOn
5 Cibelli, J. B., Stice, S. L., Golueke, P. J., Kane, J. J., Jerry, J., Blackwell, C., Ponce de Leon, F. A. and Robl, J. M. 1998. Cloned transgenic calves produced from non-quiescent fetal fibroblasts. Sci. 280:1256-1258.   DOI   ScienceOn
6 Hultdin, M., Gronlund, E., Norrback, K., ErikassonLindstrom, E., Just, T. and Roos, G. 1998. Telomere analysis by fluorescence in situ hybridization and flow cytometry. Nucleic Acids Res. 26(16):3651-3656.
7 ISCNDA 1990. International system for cytogenetic nomenclature of domestic animals. Cytogenet. Cell Genet. 53:65-79.
8 Meeker, A K and Coffey, D. S. 1997. Telomerase: a promising marker of biological immortality of germ, stem, and cancer cells. A review. Biochemistry 62(11): 1323-1331.
9 Miyashita, N., Shiga, K., Yonai, M., Kaneyama, K., Kobayashi, S., Kojima, T., Goto, Y., Kishi, M., Aso, H., Suzuki, T., Sakaguchi, M. and Nagai, T. 2002. Remarkable difference in telomere lengths among cloned cattle derived from different cell types. BioI. Reprod. 66:1649-1655.
10 Multani, AS., Ozen, M., Narayan, S., Kumar, V., Chandra, J., McConkey, D. J., Newman, R A and Pathak, S. 2000. Caspase-dependent apoptosis induced by telomere cleavage and TRF2 loss. Neoplasia 2(4):339-45.
11 Yamaguchi, Y., Nozawa, K., Savoysky, E., Hayakawa, N., Nimura, Y. and Yoshida, S. 1998. Change in telomerase activity of rat organs during growth and aging. Exptl. Cell Res. 242:120-127.
12 Tian, X. C., Xu, J. and Yang, X. 2000. Normal telomere lengths found in cloned cattle. Nat. Genet. 26:272-273.
13 Ulaner, G. A and Giudice, L. C. 1997. Developmental regulation of telomerase activity in human fetal tissues during gestation. Mol. Hum. Reprod. 3(9):769-773.   DOI   ScienceOn
14 Wilmut, I., Schnieke, A. E., McWhir, J., Kind, A. J. and Campbell, K. H. 1997. Viable offspring derived from fetal and adult mammalian cells. Nature 385:810-813.   DOI   ScienceOn
15 손시환, 이재익. 1998. 고분염분석(High-resolution banding)에 의한 한우 염색체의 표준표지 설정. 한국축산학회지 40(5):467-484.
16 Gan, Y., Engelke, K. J., Brown, C. A. and Au, J. L. 2001. Telomere amount and length assay. Pharma. Res. 18(12):1655-1659.   DOI   ScienceOn
17 Cottliar, A. S. and Slavutsky, I. R. 2001. Telomeres and telomerase activity: their role in aging and in neoplastic development. Medicina 61:335-342.
18 de la Sena, C., Chowdhary, B. P. and Gustavsson, I. 1995. Localization of telomeric(TTAGGG)n sequences in chromosomes of some domestic animals by fluorescence in situ hybridization. Hereditas 123:269-274.
19 Fradiani, P. A., Ascenzioni, F., Lavitrano, M. and Donini, P. 2004. Telomeres and telomerase activity in pig tissues. Biochimie. 86:7-12.
20 Greider, C. W. and Blackburn, E. H. 1985. Identification of a specific telomere terminal transferase activity in Tetrahymena extracts. Cell 43:405-413.   DOI   ScienceOn
21 Gu, F., Hindkjaer, J., Gustavsson, I. and Bolund, L. 1996. A signal of telomeric sequences on porcine chromosome 6q21 ~q22 detected by primed in situ labelling. Chromosome Res. 4:251-252.
22 Poon, S. S., Martens, U. M., Ward, R. K. and Lansdorp, P. M. 1999. Telomere length measurements using digital fluorescence microscopy. Cytometry 36:267-278.   DOI   ScienceOn
23 Hemann, M. T., Strong, M. A, Hao, L. Y. and Greider, C. W. 2001. The shortest telomere, not average telomere length, is critical for cell viability and chromosome stability. Cell 107:67-77.   DOI   ScienceOn
24 Onishi, A., Iwamoto, M., Akita, T., Mikawa, S., Takeda, K, Awata, T., Hanada, H. and Perry, A. C. 2000. Pig cloning by microinjection of fetal fibroblast nuclei. Sci. 289:1188-1190.   DOI   ScienceOn
25 Pathak, S., Mutani, A S., Furlong, C. L. and Sohn, S. H. 2002. Telomere dynamics, aneuploidy, stem cells and cancer. Int. J. Oncology 20(3):637-641.
26 Robinson, M. O. 2000. Telomerase and cancer. Genet. Eng. 22:209-222.
27 Shiels, P. G., Kind, A. J., Campbell, K. H., Waddington, D., Wilmut, I., Colman, A. and Schnieke, A. E. 1999. Analysis of telomere lengths in cloned sheep. Nature 399:316-317.