[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5851/kosfa.2021.e53

Effect of Pesticide Residue in Muscle and Fat Tissue of Pigs Treated with Propiconazole

Jeong, Jin Young (Animal Nutrition & Physiology Division, National Institute of Animal Science, Rural Development Administration)
Kim, Byeonghyeon (Animal Nutrition & Physiology Division, National Institute of Animal Science, Rural Development Administration)
Ji, Sang Yun (Animal Nutrition & Physiology Division, National Institute of Animal Science, Rural Development Administration)
Baek, Youl Chang (Animal Nutrition & Physiology Division, National Institute of Animal Science, Rural Development Administration)
Kim, Minji (Animal Nutrition & Physiology Division, National Institute of Animal Science, Rural Development Administration)
Park, Seol Hwa (Animal Nutrition & Physiology Division, National Institute of Animal Science, Rural Development Administration)
Kim, Ki Hyun (Animal Welfare Research Team, National Institute of Animal Science, Rural Development Administration)
Oh, Sang-Ik (Division of Animal Disease & Health, National Institute of Animal Science, Rural Development Administration)
Kim, Eunju (Division of Animal Disease & Health, National Institute of Animal Science, Rural Development Administration)
Jung, Hyunjung (Animal Nutrition & Physiology Division, National Institute of Animal Science, Rural Development Administration)

Publication Information

Food Science of Animal Resources / v.41, no.6, 2021 , pp. 1022-1035 More about this Journal

Abstract

This study estimated the effect of exposure to propiconazole through implementation and residues in finishing pigs. We analyzed the expression of fibrosis-related genes and performed histological analysis of the blood, liver, kidney, muscle, ileum, and fat tissues. The animals were exposed for 28 d to different concentrations of propiconazole (0.09, 0.44, 0.88, 4.41, and 8.82 mg/kg bw/d). Quantitative, gene expression, and histological analyses in tissues were performed using liquid chromatography mass spectrometry, real-time PCR, and Masson's trichrome staining, respectively. Final body weight did not differ among groups. However, genes involved in fibrosis were significantly differentially regulated in response to propiconazole concentrations. Glucose, alanine aminotransferase, and total bilirubin levels were significantly increased compared with those in the control group, while alkaline phosphatase level was decreased (p<0.05) after exposure to propiconazole. The residue limits of propiconazole were increased in the finishing phase at 4.41 and 8.82 mg/kg bw/d. The liver, kidney, and ileum showed blue staining after propiconazole treatment, confirmed by Masson's trichrome staining. In conclusion, these findings suggest that propiconazole exposure disturbs the expression of fibrosis-related genes. This study on dietary propiconazole in pigs can provide a basis for determining maximum residue limits and a better understanding of metabolism in pigs and meat products.

Keywords

pig; propiconazole; fibrosis;

Citations & Related Records

Reference

1	Hester SD, Wolf DC, Nesnow S, Thai SF. 2006. Transcriptional profiles in liver from rats treated with tumorigenic and non-tumorigenic triazole conazole fungicides: Propiconazole, triadimefon, and myclobutanil. Toxicol Pathol 34:879-894. DOI
2	Sun G, Thai SF, Tully DB, Lambert GR, Goetz AK, Wolf DC, Dix DJ, Nesnow S. 2005. Propiconazole-induced cytochrome P450 gene expression and enzymatic activities in rat and mouse liver. Toxicol Lett 155:277-287. DOI
3	Tao R, Fan XX, Yu HJ, Ai G, Zhang HY, Kong HY, Song QQ, Huang Y, Huang JQ, Ning Q. 2018. MicroRNA-29b-3p prevents Schistosoma japonicum-induced liver fibrosis by targeting COL1A1 and COL3A1. J Cell Biochem 119:3199-3209. DOI
4	Van den Bosch F, Oliver R, van den Berg F, Paveley N. 2014. Governing principles can guide fungicide-resistance management tactics. Annu Rev Phytopathol 52:175-195. DOI
5	Velisek J, Stara A, Li ZH, Silovska S, Turek J. 2011. Comparison of the effects of four anaesthetics on blood biochemical profiles and oxidative stress biomarkers in rainbow trout. Aquac 310: 369-375. DOI
6	VoPham T, Bertrand KA, Hart JE, Laden F, Brooks MM, Yuan JM, Talbott EO, Ruddell D, Chang CCH, Weissfeld JL. 2017. Pesticide exposure and liver cancer: a review. Cancer Causes Control 28:177-190. DOI
7	Allen JW, Wolf DC, George MH, Hester SD, Sun G, Thai SF, Delker DA, Moore T, Jones C, Nelson G, Roop BC, Leavitt S, Winkfield E, Ward WO, Nesnow S. 2006. Toxicity profiles in mice treated with hepatotumorigenic and non-hepatotumorigenic triazole conazole fungicides: Propiconazole, triadimefon, and myclobutanil. Toxicol Pathol 34:853-862. DOI
8	Bennewith KL, Huang X, Ham CM, Graves EE, Erler JT, Kambham N, Feazell J, Yang GP, Koong A, Giaccia AJ. 2009. The role of tumor cell-derived connective tissue growth factor (CTGF/CCN2) in pancreatic tumor growth. Cancer Res 69:775-784. DOI
9	Brittain C, Potts SG. 2011. The potential impacts of insecticides on the life-history traits of bees and the consequences for pollination. Basic Appl Ecol 12:321-331. DOI
10	Eid RA. 2017. Apoptosis of rat renal cells by organophosphate pesticide, quinalphos: Ultrastructural study. Saudi J Kidney Dis Transpl 28:725-736.
11	George B, You D, Joy MS, Aleksunes LM. 2017. Xenobiotic transporters and kidney injury. Adv Drug Deliv Rev 116:73-91. DOI
12	Godin RE, Takaesu NT, Robertson EJ, Dudley AT. 1998. Regulation of BMP7 expression during kidney development. Development 125:3473-3482. DOI
13	Hester S, Moore T, Padgett WT, Murphy L, Wood CE, Nesnow S. 2012. The hepatocarcinogenic conazoles: Cyproconazole, epoxiconazole, and propiconazole induce a common set of toxicological and transcriptional responses. Toxicol Sci 127:54-65. DOI
14	Li ZH, Velisek J, Grabic R, Li P, Kolarova J, Randak T. 2011. Use of hematological and plasma biochemical parameters to assess the chronic effects of a fungicide propiconazole on a freshwater teleost. Chemosphere 83:572-578. DOI
15	Huang Y, Yu Y, Zhan S, Tomberlin JK, Huang D, Cai M, Zheng L, Yu Z, Zhang J. 2020. Dual oxidase Duox and Toll-like receptor 3 TLR3 in the Toll pathway suppress zoonotic pathogens through regulating the intestinal bacterial community homeostasis in Hermetia illucens L. PLOS ONE 15:e0225873. DOI
16	Alengebawy A, Abdelkhalek ST, Qureshi SR, Wang MQ. 2021. Heavy metals and pesticides toxicity in agricultural soil and plants: Ecological risks and human health implications. Toxics 9:42. DOI
17	Uitto J, Kouba D. 2000. Cytokine modulation of extracellular matrix gene expression: Relevance to fibrotic skin diseases. J Dermatol Sci Suppl 1:S60-S69.
18	Igbedioh SO. 1991. Effects of agricultural pesticides on humans, animals, and higher plants in developing countries. Arch Environ Health 46:218-224. DOI
19	Karabelas AJ, Plakas KV, Solomou ES, Drossou V, Sarigiannis DA. 2009. Impact of European legislation on marketed pesticides: A view from the standpoint of health impact assessment studies. Environ Int 35:1096-1107. DOI
20	Klink H, Verreet JA, Hasler M, Birr T. 2021. Will triazoles still be of importance in disease control of Zymoseptoria tritici in the future? Agronomy 11:933. DOI
21	Firat O, Cogun HY, Yuzereroglu TA, Gok G, Firat O, Kargin F, Kotemen Y. 2011. A comparative study on the effects of a pesticide (cypermethrin) and two metals (copper, lead) to serum biochemistry of Nile tilapia, Oreochromis niloticus. Fish Physiol Biochem 37:657-666. DOI
22	Ivkovic S, Yoon BS, Popoff SN, Safadi FF, Libuda DE, Stephenson RC, Daluiski A, Lyons KM. 2003. Connective tissue growth factor coordinates chondrogenesis and angiogenesis during skeletal development. Development 130:2779-2791. DOI
23	Costa NO, Vieira ML, Sgarioni V, Pereira MRF, Montagnini BG, Mesquita SFP, Gerardin DCC. 2015. Evaluation of the reproductive toxicity of fungicide propiconazole in male rats. Toxicology 335:55-61. DOI
24	Manson SR, Austin PF, Guo Q, Moore KH. 2015. BMP-7 signaling and its critical roles in kidney development, the responses to renal injury, and chronic kidney disease. Vitam Horm 99:91-144. DOI
25	Naik PK, Bozyk PD, Bentley JK, Popova AP, Birch CM, Wilke CA, Fry CD, White ES, Sisson TH, Tayob N, Carnemolla B, Orecchia P, Flaherty KR, Hershenson MB, Murray S, Martinez FJ, Moore BB, COMET Investigators. 2012. Periostin promotes fibrosis and predicts progression in patients with idiopathic pulmonary fibrosis. Am J Physiol Lung Cell Mol Physiol 303:L1046-L1056. DOI
26	Seralini GE, Cellier D, Spiroux de Vendomois J. 2007. New analysis of a rat feeding study with a genetically modified maize reveals signs of hepatorenal toxicity. Arch Environ Contam Toxicol 52:596-602. DOI
27	Steiner D, Krska R, Malachova A, Taschl I, Sulyok M. 2020. Evaluation of matrix effects and extraction efficiencies of LC-MS/MS methods as the essential part for proper validation of multiclass contaminants in complex feed. J Agric Food Chem 68:3868-3880. DOI
28	Lenz B, Braendli-Baiocco A, Engelhardt J, Fant P, Fischer H, Francke S, Fukuda R, Groters S, Harada T, Harleman H, Kaufmann W, Kustermann S, Nolte T, Palazzi X, Pohlmeyer-Esch G, Popp A, Romeike A, Schulte A, Silva Lima B, Tomlinson L, Willard J, Wood CE, Yoshida M. 2018. Characterizing adversity of lysosomal accumulation in nonclinical toxicity studies: Results from the 5th ESTP International Expert Workshop. Toxicol Pathol 46:224-246. DOI
29	Arnal N, Astiz M, de Alaniz MJT, Marra CA. 2011. Clinical parameters and biomarkers of oxidative stress in agricultural workers who applied copper-based pesticides. Ecotoxicol Environ Saf 74:1779-1786. DOI
30	Chen CC, Chen N, Lau LF. 2001. The angiogenic factors Cyr61 and connective tissue growth factor induce adhesive signalling in primary human skin fibroblasts. J Biol Chem 276:10443-10452. DOI
31	Kwon HC, Sohn H, Kim DH, Shin DM, Jeong CH, Chang YH, Yune JH, Kim YJ, Kim DW, Kim SH, Han SG. 2021. In vitro and in vivo study on the toxic effects of propiconazole fungicide in the pathogenesis of liver fibrosis. J Agric Food Chem 69:7399-7408. DOI
32	Kim WR, Flamm SL, Bisceglie AMD, Bodenheimer HC Jr. 2008. Serum activity of alanine aminotransferase (ALT) as an indicator of health and disease. Hepatology 47:1363-1370. DOI
33	Herrera J, Forster C, Pengo T, Montero A, Swift J, Schwartz MA, Henke CA, Bitterman PB. 2019. Registration of the extracellular matrix components constituting the fibroblastic focus in idiopathic pulmonary fibrosis. JCI Insight 4:e125185. DOI
34	Horiuchi K, Amizuka N, Takeshita S, Takamatsu H, Katsuura M, Ozawa H, Toyama Y, Bonewald LF, Kudo A. 1999. Identification and characterization of a novel protein, periostin, with restricted expression to periosteum and periodontal ligament and increased expression by transforming growth factor beta. J Bone Miner Res 14:1239-1249. DOI
35	Jeong J, Kwon EG, Im SK, Seo KS, Baik M. 2012. Expression of fat deposition and fat removal genes is associated with intramuscular fat content in longissimus dorsi muscle of Korean cattle steers. J Anim Sci 90:2044-2053. DOI
36	Kendall RT, Feghali-Bostwick CA. 2014. Fibroblasts in fibrosis: Novel roles and mediators. Front Pharmacol 5:123. DOI
37	Lushchak VI, Matviishyn TM, Husak VV, Storey JM, Storey KB. 2018. Pesticide toxicity: A mechanistic approach. EXCLI J 17:1101-1136.
38	Mamane A, Raherison C, Tessier JF, Baldi I, Bouvier G. 2015. Environmental exposure to pesticides and respiratory health. Eur Respir Rev 24:462-473. DOI
39	Meng XM, Tang PMK, Li J, Lan HY. 2015. TGF-β/Smad signaling in renal fibrosis. Front Physiol 6:82. DOI
40	Moser VC, Macphail RC. 1989. Neurobehavioral effects of triadimefon, a triazole fungicide, in male and female rats. Neurotoxicol Teratol 11:285-293. DOI
41	Sharma P, Soni K, Guttikar S, Singhal P, Patel DP, Shrivastav PS. 2012. Separation of all-trans- and 13-cis-isomers of acitretin on a reversed-phase column, optimization of stability conditions and their simultaneous quantitation in human plasma by liquid chromatography-tandem mass spectrometry. Anal Methods 4:791-806. DOI
42	Yang C, Song G, Lim W. 2020. Effects of endocrine disrupting chemicals in pigs. Environ Pollut 263:114505. DOI
43	Verrecchia F, Mauviel A. 2007. Transforming growth factor-beta and fibrosis. World J Gastroenterol 13:3056-3062. DOI
44	Wang H, Ran J, Jiang T. 2014. Urea. Subcell Biochem 73:7-29. DOI
45	Qu D, Zhou X, Yang F, Tian S, Zhang X, Ma L, Han J. 2017. Development of class model based on blood biochemical parameters as a diagnostic tool of PSE meat. Meat Sci 128:24-29. DOI
46	Ramani S, Recharla N, Hwang O, Jeong J, Park S. 2021. Meta-analysis identifies the effect of dietary multi-enzyme supplementation on gut health of pigs. Sci Rep 11:7299. DOI
47	Sharma U, Pal D, Prasad R. 2014. Alkaline phosphatase: An overview. Indian J Clin Biochem 29:269-278. DOI
48	Wang Q, Usinger W, Nichols B, Gray J, Xu L, Seeley TW, Brenner M, Guo G, Zhang W, Oliver N, Lin A, Yeowell D. 2011. Cooperative interaction of CTGF and TGF-β in animal models of fibrotic disease. Fibrogenesis Tissue Repair 4:4. DOI