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http://dx.doi.org/10.5851/kosfa.2020.e49

Genetic Analysis and Characterization of a Bacteriophage ØCJ19 Active against Enterotoxigenic Escherichia coli  

Kim, Gyeong-Hwuii (Department of Biological Science and Technology, Yonsei University)
Kim, Jae-Won (Institute of Biotechnology, CJ CheilJedang)
Kim, Jaegon (Department of Biological Science and Technology, Yonsei University)
Chae, Jong Pyo (Institute of Biotechnology, CJ CheilJedang)
Lee, Jin-Sun (Department of Biological Science and Technology, Yonsei University)
Yoon, Sung-Sik (Department of Biological Science and Technology, Yonsei University)
Publication Information
Food Science of Animal Resources / v.40, no.5, 2020 , pp. 746-757 More about this Journal
Abstract
Enterotoxigenic Escherichia coli (ETEC) is the major pathogenic E. coli that causes diarrhea and edema in post-weaning piglets. In this study, we describe the morphology and characteristics of ØCJ19, a bacteriophage that infects ETEC, and performed genetic analysis. Phage ØCJ19 belongs to the family Myoviridae. One-step growth curve showed a latent phase of 5 min and burst size of approximately 20 phage particles/infected cell. Phage infectivity was stable for 2 h between 4℃ and 55℃, and the phage was stable between pH 3 and 11. Genetic analysis revealed that phage ØCJ19 has a total of 49,567 bases and 79 open reading frames (ORFs). The full genomic sequence of phage ØCJ19 showed the most similarity to an Escherichia phage, vB_EcoS_ESCO41. There were no genes encoding lysogeny, toxins, virulence factors, or antibiotic resistance in this phage, suggesting that this phage can be used safely as a biological agent to control ETEC. Comparative genomic analysis in terms of the tail fiber proteins could provide genetic insight into host recognition and the relationship with other coliphages. These results showed the possibility to improve food safety by applying phage ØCJ19 to foods of animal origin contaminated with ETEC and suggests that it could be the basis for establishing a safety management system in the animal husbandry.
Keywords
animal husbandry; animal source food; bacteriophage; enterotoxigenic Escherichia coli; genome annotation;
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1 Mohammed M, Cormican M. 2015. Whole genome sequencing provides possible explanations for the difference in phage susceptibility among two Salmonella Typhimurium phage types (DT8 and DT30) associated with a single foodborne outbreak. BMC Res Notes 8:728.   DOI
2 O'Flynn G, Ross RP, Fitzgerald GF, Coffey A. 2004. Evaluation of a cocktail of three bacteriophages for biocontrol of Escherichia coli O157:H7. Appl Environ Microbiol 70:3417-3424.   DOI
3 Pattabiraman V, Katz LS, Chen JC, McCullough AE, Trees E. 2018. Genome wide characterization of enterotoxigenic Escherichia coli serogroup O6 isolates from multiple outbreaks and sporadic infections from 1975-2016. PLOS ONE 13:e0208735.   DOI
4 Peng Q, Yuan Y. 2018. Characterization of a newly isolated phage infecting pathogenic Escherichia coli and analysis of its mosaic structural genes. Sci Rep 8:8086.   DOI
5 Romero-Calle D, Guimaraes BR, Goes-Neto A, Billington C. 2019. Bacteriophages as alternatives to antibiotics in clinical care. Antibiotics (Basel) 8:138.   DOI
6 Simoliunas E, Kaliniene L, Stasilo M, Truncaite L, Zajanckauskaite A, Staniulis J, Nainys J, Kaupinis A, Valius M, Meskys R. 2014. Isolation and characterization of vB_ArS-ArV2-First Arthrobacter sp. infecting bacteriophage with completely sequenced genome. PLOS ONE 9:e111230.   DOI
7 Wei C, Zhao X. 2018. Induction of viable but nonculturable Escherichia coli O157:H7 by low temperature and its resuscitation. Front Microbiol 9:2728.   DOI
8 Yan X, Huang X, Ren J, Zou Z, Yang S, Ouyang J, Zeng W, Yang B, Xiao S, Huang L. 2009. Distribution of Escherichia coli F4 adhesion phenotypes in pigs of 15 Chinese and Western breeds and a White Duroc$\times$ Erhualian intercross. J Med Microbiol 58:1112-1117.   DOI
9 Yoichi M, Abe M, Miyanaga K, Unno H, Tanji Y. 2005. Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157:H7. J Biotechnol 115:101-107.   DOI
10 Yang SC, Lin CH, Aljuffali IA, Fang JY. 2017. Current pathogenic Escherichia coli foodborne outbreak cases and therapy development. Arch Microbiol 199:811-825.   DOI
11 Yosef I, Goren MG, Globus R, Molshanski-Mor S, Qimron U. 2017. Extending the host range of bacteriophage particles for DNA transduction. Mol Cell 66:721-728.   DOI
12 Zhou Y, Bao H, Zhang H, Wang R. 2015. Isolation and characterization of lytic phage vB_EcoM_JS09 against clinically isolated antibiotic-resistant avian pathogenic Escherichia coli and enterotoxigenic Escherichia coli. Intervirology 58:218-231.   DOI
13 Abedon ST, Herschler TD, Stopar D. 2001. Bacteriophage latent-period evolution as a response to resource availability. Appl Environ Microb 67:4233-4241.   DOI
14 Al-Gallas N, Bahri O, Bouratbeen A, Haasen AB, Aissa RB. 2007. Etiology of acute diarrhea in children and adults in Tunis, Tunisia, with emphasis on diarrheagenic Escherichia coli: Prevalence, phenotyping, and molecular epidemiology. Am J Trop Med Hyg 77:571-582.   DOI
15 Alves DR, Gaudion A, Bean JE, Esteban PP, Arnot TC, Harper DR, Kot W, Enright MC, Jenkins ATA. 2014. Combined use of bacteriophage K and a novel bacteriophage to reduce Staphylococcus aureus biofilm formation. Appl Environ Microbiol 80:6694-6703.   DOI
16 Bai J, Kim YT, Ryu S, Lee JH. 2016. Biocontrol and rapid detection of food-borne pathogens using bacteriophages and endolysins. Front Microbiol 7:474.
17 Abedon ST, Hyman P, Thomas C. 2003. Experimental examination of bacteriophage latent-period evolution as a response to bacterial availability. Appl Environ Microb 69:7499-7506.   DOI
18 Chang Y, Bai J, Lee JH, Ryu S. 2019. Mutation of a Staphylococcus aureus temperate bacteriophage to a virulent one and evaluation of its application. Food Microbiol 82:523-532.   DOI
19 Bao H, Zhang P, Zhang H, Zhou Y, Zhang L, Wang R. 2015. Bio-control of Salmonella enteritidis in foods using bacteriophages. Viruses 7:4836-4853.   DOI
20 Cao Z, Zhang J, Niu YD, Cui N, Ma Y, Cao F, Xu Y. 2015. Isolation and characterization of a "phiKMV-Like" bacteriophage and its therapeutic effect on mink hemorrhagic pneumonia. PLOS ONE 10:e0116571.   DOI
21 Fabian NJ, Mannion AJ, Feng Y, Madden CM, Fox JG. 2020. Intestinal colonization of genotoxic Escherichia coli strains encoding colibactin and cytotoxic necrotizing factor in small mammal pets. Vet Microbiol 240:108506.   DOI
22 Cheng G, Hao H, Xie S, Wang X, Dai M. Huang,L, Yuan Z. 2014. Antibiotic alternatives: The substitution of antibiotics in animal husbandry? Front Microbiol 5:217.   DOI
23 De Souza GM, Neto ERDS, da Silva AM, de Souza Iacia MVM, Rodrigues MVP, Pereira VC, Winkelstroter LK. 2019. Comparative study Of genetic diversity, virulence genotype, biofilm formation and antimicrobial resistance of Uropathogenic Escherichia coli (UPEC) isolated from nosocomial and community acquired urinary tract infections. Infect Drug Resist 12:3595-3606.   DOI
24 Endersen L, O'Mahony J, Hill C, Ross RP, McAuliffe O, Coffey A. 2014. Phage therapy in the food industry. Annu Rev Food Sci Technol 5:327-349.   DOI
25 Gutierrez D, Rodriguez-Rubio L, Fernandez L, Martinez B, Rodriguez A, Garcia P. 2017. Applicability of commercial phage-based products against Listeria monocytogenes for improvement of food safety in Spanish dry-cured ham and food contact surfaces. Food Control 73:1474-1482.   DOI
26 Kim GH, Kim J, Kim KH, Lee JS, Lee NG, Lim TH, Yoon SS. 2019. Characterization and genomic analysis of novel bacteriophage ${\Phi}CS01$ targeting Cronobacter sakazakii. J Microbiol Biotechnol 29:696-703.   DOI
27 Hesse S, Adhya S. 2019. Phage therapy in the twenty-first century: Facing the decline of the antibiotic era; is it finally time for the age of the phage? Annu Rev Microbiol 73:155-174.   DOI
28 Jamalludeen N, Johnson RP, Friendship R, Kropinski AM, Lingohr EJ, Gyles CL. 2007. Isolation and characterization of nine bacteriophages that lyse O149 enterotoxigenic Escherichia coli. Vet Microbiol 124:47-57.   DOI
29 Jang J, Hur HG, Sadowsky MJ, Byappanahalli MN, Yan T, Ishii S. 2017. Environmental Escherichia coli: Ecology and public health implications-a review. J Appl Microbiol 123:570-581.   DOI
30 Kebriaei R, Lev K, Morrisette T, Stamper KC, Abdul-Mutakabbir JC, Lehman SM, Morales S, Rybak MJ. 2020. Bacteriophage-antibiotic combination strategy: An alternative against methicillin-resistant phenotypes of Staphylococcus aureus. Antimicrob Agents Chemother 64:e00461-20.
31 Kim J, Kim GH, Lee NG, Lee JS, Yoon SS. 2018. Whole-genome sequencing and genomic analysis of a virulent bacteriophage infecting Bacillus cereus. Intervirology 61:272-280.   DOI
32 Laxminarayan R, Duse A, Wattal C, Zaidi AKM, Wertheim HFL, Sumpradit N, Vlieghe E, Hara GL, Gould IM, Goossens H, Greko C, D So A, Bigdeli M, Tomson G, Woodhouse W, Ombaka E, Peralta AQ, Qamar FN, Mir F, Kariuki S, Bhutta ZA, Coates A, Bergstrom R, Wright GD, Brown ED, Cars O. 2013. Antibiotic resistance- the need for global solutions. Lancet Infect Dis 13:1057-1098.   DOI
33 Lee CY, Kim SJ, Park BC, Han JH. 2017. Effects of dietary supplementation of bacteriophages against enterotoxigenic Escherichia coli (ETEC) K88 on clinical symptoms of post-weaning pigs challenged with the ETEC pathogen. J Anim Physiol Anim Nutr 101:88-95.   DOI
34 Mayo MA, Haenni AL. 2006. Report from the 36th and the 37th meetings of the executive committee of the international committee on taxonomy of viruses. Arch Virol 151:1031-1037.   DOI
35 Lee HJ, Kim WI, Kwon YC, Cha KE, Kim M, Myung H. 2016. A Newly isolated bacteriophage, PBES 02, infecting Cronobacter sakazakii. J Microbiol Biotechnol 26:1629-1635.   DOI
36 Luppi A. 2017. Swine enteric colibacillosis: Diagnosis, therapy and antimicrobial resistance. Porcine Health Manag 3:16.   DOI