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http://dx.doi.org/10.5851/kosfa.2019.e93

Duck Oil-loaded Nanoemulsion Inhibits Senescence of Angiotensin II-treated Vascular Smooth Muscle Cells by Upregulating SIRT1  

Kang, Eun Sil (Department of Food Science and Biotechnology of Animal Resources, College of Sang-Huh Life Science, Konkuk University)
Kim, Hyo Juong (Taekyung Food and Processing R&D Center)
Han, Sung Gu (Department of Food Science and Biotechnology of Animal Resources, College of Sang-Huh Life Science, Konkuk University)
Seo, Han Geuk (Department of Food Science and Biotechnology of Animal Resources, College of Sang-Huh Life Science, Konkuk University)
Publication Information
Food Science of Animal Resources / v.40, no.1, 2020 , pp. 106-117 More about this Journal
Abstract
Cellular senescence is associated with age-related vascular disorders and has been implicated in vascular dysfunctions. Here, we show that duck oil-loaded nanoemulsion (DO-NE) attenuates premature senescence of vascular smooth muscle cells (VSMCs) triggered by angiotensin II (Ang II). Compared with control nanoemulsion (NE), DO-NE significantly inhibited the activity of senescence-associated β-galactosidase, which is a biomarker of cellular senescence, in Ang II-treated VSMCs. SIRT1 protein expression was dose- and time-dependently induced in VSMCs exposed to DO-NE, but not in those exposed to NE, and SIRT1 promoter activity was also elevated. Consistently, DO-NE also dose-dependently rescued Ang II-induced repression of SIRT1 expression, indicating that SIRT1 is linked to the anti-senescence action of DO-NE in VSMCs treated with Ang II. Furthermore, the SIRT1 agonist resveratrol potentiated the effects of DO-NE on VSMCs exposed to Ang II, whereas the SIRT1 inhibitor sirtinol elicited the opposite effect. These findings indicate that DO-NE inhibits senescence by upregulating SIRT1 and thereby impedes vascular aging triggered by Ang II.
Keywords
angiotensin II; duck oil; nanoemulsion; senescence; SIRT1;
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Times Cited By KSCI : 2  (Citation Analysis)
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