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http://dx.doi.org/10.5851/kosfa.2019.e91

Enzymatic Hydrolysis of Egg White Protein Exerts a Hypotensive Effect in Spontaneously Hypertensive Rats  

Lee, Da-Eon (Department of Food and Biotechnology, Sahmyook University)
Jung, Tae-Hwan (Convergence Research Center, Sahmyook University)
Jo, Yu-Na (Department of Food and Biotechnology, Sahmyook University)
Yun, Sung-Seob (R&D Department, Bioprofoods Co. Ltd.)
Han, Kyoung-Sik (Department of Food and Biotechnology, Sahmyook University)
Publication Information
Food Science of Animal Resources / v.39, no.6, 2019 , pp. 980-987 More about this Journal
Abstract
This study was conducted to investigate the hypotensive effect of egg white protein (EWP) hydrolysate (EWH) in spontaneously hypertensive rats (SHRs). The hydrolysis of EWP was effectively performed with a combination of 0.5% bromelain and 1% papain at 50℃ for 60 min. The resulting hydrolysate did not elicit an allergic reaction as confirmed by human mast cell activation test. The systolic and diastolic blood pressures of the SHRs fed the EWH diet were observed to be significantly or numerically lower than those of the other groups during the experimental period of 28 d. EWH treatment significantly (p<0.05) upregulated the nitric oxide levels in hCMEC/D3 cells and the plasma of the SHRs compared to those in the control. Moreover, EWH ingestion significantly (p<0.01) reduced the plasma angiotensin II level of the SHRs compared with that in the control. In conclusion, beyond its basic nutritional value, EWH prevents and manages hypertension, and thus can be an invaluable resource for functional food development.
Keywords
egg white protein hydrolysate; spontaneously hypertensive rats; hypotensive effect; nitric oxide; angiotensin II;
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1 Okamoto K, Aoki K. 1963. Development of a strain of spontaneously hypertensive rats. Jpn Circ J 27:282-293.   DOI
2 Phelan M, Kerins D. 2011. The potential role of milk-derived peptides in cardiovascular disease. Food Funct 2:153-167.   DOI
3 Rajapakse NW, Head GA, Kaye DM. 2016. Say NO to obesity-related hypertension: Role of the l-Arginine-Nitric oxide pathway. Hypertension 67:813-819.   DOI
4 Viera AJ. 2012. Resistant hypertension. J Am Board Fam Med 25:487-495.   DOI
5 Wu J, Liao W, Udenigwe CC. 2017. Revisiting the mechanisms of ACE inhibitory peptides from food proteins. Trends Food Sci Technol 69:214-219.   DOI
6 Aluko RE. 2015. Antihypertensive peptides from food proteins. Annu Rev Food Sci Technol 6:235-262.   DOI
7 Chockalingam A. 2008. World hypertension day and global awareness. Can J Cardiol 24:441-444.   DOI
8 Behrendt D, Ganz P. 2002. Endothelial function. From vascular biology to clinical applications. Am J Cardiol 90:40L-48L.   DOI
9 Boonla O, Kukongviriyapan U, Pakdeechote P, Kukongviriyapan V, Pannangpetch P, Thawornchinsombut S. 2015. Peptidesderived from thai rice bran improves endothelial function in 2K-1C renovascular hypertensive rats. Nutrients 7:5783-5799.   DOI
10 Chen S, Chen G, Shu S, Xu Y, Ma X. 2019. Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats. R Soc Open Sci 6:181081.   DOI
11 Danaei G, Singh GM, Paciorek CJ, Lin JK, Cowan MJ, Finucane MM, Farzadfar F, Stevens GA, Riley LM, Lu Y, Rao M, Ezzati M. 2013. The global cardiovascular risk transition: Associations of four metabolic risk factors with national income, urbanization, and western diet in 1980 and 2008. Circulation 127:1493-1502.   DOI
12 Davalos A, Miguel M, Bartolome B, Lopez-Fandino R. 2004. Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Prot 67:1939-1944.   DOI
13 Duan X, Wu F, Li M, Yang N, Wu C, Jin Y, Yang Y, Jin Z, Xu X. 2014. Naturally occurring angiotensin I-converting enzyme inhibitory peptide from a fertilized egg and its inhibitory mechanism. J Agric Food Chem 62:5500-5506.   DOI
14 Endemann DH, Schiffrin EL. 2004. Endothelial dysfunction. J Am Soc Nephrol 15:1983-1992.   DOI
15 Heiss C, Rodriguez-Mateos A, Kelm M. 2015. Central role of eNOS in the maintenance of endothelial homeostasis. Antioxid Redox Signal 22:1230-1242.   DOI
16 Bader M, Ganten D. 2008. Update on tissue renin-angiotensin systems. J Mol Med 86:615-621.   DOI
17 FitzGerald RJ, Murray BA, Walsh DJ. 2004. Hypotensive peptides from milk proteins. J Nutr 134:980S-988S.   DOI
18 Ganguly A, Sharma K, Majumder K. 2019. Food-derived bioactive peptides and their role in ameliorating hypertension and associated cardiovascular diseases. Adv Food Nutr Res 89:165-207.   DOI
19 Gobbetti M, Minervini F, Rizzello CG. 2004. Angiotensin I-converting-enzyme-inhibitory and antimicrobial bioactive peptides. Int J Dairy Technol 57:173-188.   DOI
20 Grootaert C, Matthijs B, Voorspoels S, Possemiers S, Smagghe G, Van Camp J. 2017. Egg-derived bioactive peptides with ACE-inhibitory properties: A literature update. Food Funct 8:3847-3855.   DOI
21 Hermann M, Flammer A, Luscher TF. 2006. Nitric oxide in hypertension. J Clin Hypertens 8:17-29.   DOI
22 Ikarashi N, Toda T, Hatakeyama Y, Kusunoki Y, Kon R, Mizukami N, Kaneko M, Ogawa S, Sugiyama K. 2018. Antihypertensive effects of acacia polyphenol in spontaneously hypertensive rats. Int J Mol Sci 19:700.   DOI
23 Jahandideh F, Chakrabarti S, Majumder K, Li Q, Panahi S, Morton JS, Davidge ST, Wu J. 2016. Egg white protein hydrolysate reduces blood pressure, improves vascular relaxation and modifies aortic angiotensin II receptors expression in spontaneously hypertensive rats. J Funct Foods 27:667-673.   DOI
24 Manso MA, Miguel M, Even J, Hernandex R, Aleixandre A, Lopez-Fandino R. 2008. Effect of the long-term intake of an egg white hydrolysate on the oxidative status and blood lipid profile of spontaneously hypertensive rats. Food Chem 109:361-367.   DOI
25 Jahandideh F, Majumder K, Chakrabarti S, Morton JS, Panahi S, Kaufman S, Davidge ST, Wu J. 2014. Beneficial effects of simulated gastro-intestinal digests of fried egg and its fractions on blood pressure, plasma lipids and oxidative stress in spontaneously hypertensive rats. PLOS ONE 9:e115006.   DOI
26 Jung TH, Hwang HJ, Yun SS, Lee WJ, Kim JW, Ahn JY, Jeon WM, Han KS. 2017. Hypoallergenic and physicochemical properties of the A2 ${\beta}$-casein fraction of goat milk. Korean J Food Sci Anim Resour 37:940-947.   DOI
27 Khanna A, Lefkowitz L, White WB. 2008. Evaluation of recent fixed-dose combination therapies in the management of hypertension. Curr Opin Nephrol Hypertens 17:477-483.   DOI
28 Kishi T, Hirooka Y, Sakai K, Shigematsu H, Shimokawa H, Takeshita A. 2001. Overexpression of eNOS in the RVLM causes hypotension and bradycardia via GABA release. Hypertension 38:896-901.   DOI
29 Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.   DOI
30 Miguel M, Alvarez Y, Lopez-Fandino R, Alonso MJ, Salaices M. 2007. Vasodilator effects of peptides derived from egg white proteins. Regul Pept 140:131-135.   DOI
31 Miguel M, Lopez-Fandino R, Ramos M, Aleixandre A. 2005. Short-term effect of egg-white hydrolysate products on the arterial blood pressure of hypertensive rats. Br J Nutr 94:731-737.   DOI