Browse > Article
http://dx.doi.org/10.5851/kosfa.2019.e74

Manufacture and Physicochemical Properties of Chitosan Oligosaccharide/A2 β-Casein Nano-Delivery System Entrapped with Resveratrol  

Kim, Mi Young (Department of Food and Nutrition, Daegu University)
Ha, Ho-Kyung (Department of Animal Science and Technology, Sunchon National University)
Ayu, Istifiani Lola (Department of Food and Nutrition, Daegu University)
Han, Kyoung-Sik (Department of Food and Nutrition, Sahmyook University)
Lee, Won-Jae (Department of Animal Bioscience (Institute of Agriculture and Life Science), Gyeongsang National University)
Lee, Mee-Ryung (Department of Food and Nutrition, Daegu University)
Publication Information
Food Science of Animal Resources / v.39, no.5, 2019 , pp. 831-843 More about this Journal
Abstract
The purposes of this research were to form chitosan oligosaccharide (CSO)/A2 ${\beta}$-casein nano-delivery systems (NDSs) and to investigate the effects of production variables, such as CSO concentration levels (0.1%, 0.2%, and 0.3%, w/v) and manufacturing temperature ($5^{\circ}C$, $20^{\circ}C$, and $35^{\circ}C$), on the production and physicochemical characteristics of CSO/A2 ${\beta}$-casein NDSs to carry resveratrol. The morphological characteristics of CSO/A2 ${\beta}$-casein NDSs were assessed by the use of transmission electron microscopy (TEM) and particle size analyzer. High-performance liquid chromatography (HPLC) was applied to determine the entrapment efficiency (EE) of resveratrol. In the TEM images, globular-shaped particles with a diameter from 126 to 266 nm were examined implying that NDSs was successfully formed. As CSO concentration level was increased, the size and zeta-potential values of NDSs were significantly (p<0.05) increased. An increase in manufacturing temperature from $5^{\circ}C$ to $35^{\circ}C$ resulted in a significant (p<0.05) increase in the size and polydispersity index of NDSs. Over 85% of resveratrol was favorably entrapped in CSO/A2 ${\beta}$-casein NDSs. The entrapment efficiency (EE) of resveratrol was significantly (p<0.05) enhanced with an increase in manufacturing temperature while CSO concentration level did not significantly affect EE of resveratrol. There were no significant (p<0.05) changes observed in the size and polydispersity index of NDSs during heat treatments and storage in model milk and yogurt indicating that CSO/A2 ${\beta}$-casein NDSs exhibited excellent physical stability. In conclusion, the CSO concentration level and manufacturing temperature were the crucial determinants affecting the physicochemical characteristics of CSO/A2 ${\beta}$-casein NDSs containing resveratrol.
Keywords
A2 ${\beta}$-casein; chitosan oligosaccharide; nano-delivery system; resveratrol;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Ha HK, Nam GW, Khang D, Park SJ, Lee MR, Lee WJ. 2017. Development of two-step temperature process to modulate the physicochemical properties of beta-lactoglobulin nanoparticles. Korean J Food Sci Anim Resour 37:123-133.   DOI
2 Haenlein GFW. 2004. Goat milk in human nutrition. Small Ruminant Res 51:155-163.   DOI
3 Hamed I, Ozogul F, Regenstein JM. 2016. Industrial applications of crustacean by-products (chitin, chitosan, and chitooligosaccharides): A review. Trends Food Sci Technol 48:40-50.   DOI
4 Horne DS. 1998. Casein interactions: Casting light on the black boxes, the structure in dairy products. Int Dairy J 8:171-177.   DOI
5 Ishihara T, Mizushima T. 2010. Techniques for efficient entrapment of pharmaceuticals in biodegradable solid micro/nanoparticles. Expert Opin Drug Del 7:565-575.   DOI
6 Jeon YO, Lee JS, Lee HG. 2016. Improving solubility, stability, and cellular uptake of resveratrol by nanoencapsulation with chitosan and ${\gamma}$-poly (glutamic acid). Colloid Surface B Biointerfaces 147:224-233.   DOI
7 Jeong H, Samdani KJ, Yoo DH, Lee DW, Kim NH, Yoo IS, Lee JH. 2016. Resveratrol cross-linked chitosan loaded with phospholipid for controlled release and antioxidant activity. Int J Biol Macromol 93:757-766.   DOI
8 Kango S, Kalia S, Celli A, Njuguna J, Habibi Y, Kumar R. 2013. Surface modification of inorganic nanoparticles for development of organic-inorganic nanocomposites: A review. Prog Polym Sci 38:1232-1261.   DOI
9 Kumar R, Prakash KH, Cheang P, Khor KA. 2004. Temperature driven morphological changes of chemically precipitated hydroxyapatite nanoparticles. Langmuir 20:5196-5200.   DOI
10 Ahmed M, Akter MS, Lee JC, Eun JB. 2010. Encapsulation by spray drying of bioactive components, physicochemical and morphological properties from purple sweet potato. LWT-Food Sci Technol 43:1307-1312.   DOI
11 Amigo L, Fontecha J. 2011. Milk: Goat milk. In Encyclopedia of dairy sciences. 2nd ed. Fuquay JW (ed). Academic Press, London, UK. pp 484-493.
12 Bhattarai N, Gunn J, Zhang M. 2010. Chitosan-based hydrogels for controlled, localized drug delivery. Adv Drug Deliv Rev 62:83-99.   DOI
13 Anal AK, Tobiassen A, Flanagan J, Singh H. 2008. Preparation and characterization of nanoparticles formed by chitosan-caseinate interactions. Colloids Surf B Biointerfaces 64:104-110.   DOI
14 Baldrick P. 2010. The safety of chitosan as a pharmaceutical excipient. Regul Toxicol Pharmacol 56:290-299.   DOI
15 Barchar M, Mandelbaum A, Portnaya I, Perlstein H, Even-Chen S, Barenholz Y, Danino D. 2012. Development and characterization of a novel drug nanocarrier for oral delivery, based on self-assembled ${\beta}$-casein micelles. J Control Release 160:164-171.   DOI
16 Bowman K, Leong K. 2006. Chitosan nanoparticles for oral drug and gene delivery. Int J Nanomed 1:117-128.   DOI
17 Liu F, Antoniou J, Li Y, Majeed H, Liang R, Ma Y, Ma J, Zhong F. 2016. Chitosan/sulfobutylether-${\beta}$-cyclodextrin nanoparticles as a potential approach for tea polyphenol encapsulation. Food Hydrocolloid 57:291-300.   DOI
18 Lee MR, Choi HN, Ha HK, Lee WJ. 2013. Production and characterization of ${\beta}$-lactoglobulin/alginate nanoemulsion containing coenzyme $Q_{10}$: Impact of heat treatment and alginate concentrate. Korean J Food Sci Anim Resour 33:67-74.   DOI
19 Leung HW. 2001. Ecotoxicology of glutaraldehyde: Review of environmental fate and effects studies. Ecotox Environ Saf 49:26-39.   DOI
20 Li FQ, Su H, Wang J, Liu JY, Zhu QG, Fei YB, Pan YH, Hu JH. 2008. Preparation and characterization of sodium ferulate entrapped bovine serum albumin nanoparticles for liver targeting. Int J Pharm 349:274-282.   DOI
21 Lopez-Aliaga I, Diaz-Castro J, Alferez MJM, Barrionuevo M, Campos MS. 2010. A review of the nutritional and health aspects of goat milk in cases of intestinal resection. Dairy Sci Technol 90:611-622.   DOI
22 Mohanraj VJ, Chen Y. 2006. Nanoparticles: A review. Trop J Pharm Res 5:561-573.
23 Pangeni R, Sahni JK, Ali J, Sharma S, Baboota S. 2014. Resveratrol: Review on therapeutic potential and recent advances in drug delivery. Expert Opin Drug Del 11:1285-1298.   DOI
24 Park YW, Juarez M, Ramos M, Haenlein GFW. 2007. Physico-chemical characteristics of goat and sheep milk. Small Ruminant Res 68:88-113.   DOI
25 Priyadarshini P, Mishra C, Mishra B, Swain K, Rout M, Mishra SP. 2018. Impact of milk protein on human health: A1 verses A2. Int J Chem Stud 6:531-535.
26 Wenzel E, Somoza V. 2005. Metabolism and bioavailability of trans-resveratrol. Mol Nutr Food Res 49:472-481.   DOI
27 Ron N, Zimet P, Bargarum J, Livney YD. 2010. ${\beta}$-Lactoglobulin-polysaccharide complexes as nanovehicles for hydrophobic nutraceuticals in non-fat foods and clear beverages. Int Dairy J 20:686-693.   DOI
28 Sanna V, Roggio AM, Siliani S, Piccinini M, Marceddu S, Mariani A, Sechi M. 2012. Development of novel cationic chitosan-and anionic alginate-coated poly (D,L-lactide-co-glycolide) nanoparticles for controlled release and light protection of resveratrol. Int Nanomed 7:5501-5516.
29 Summerlin N, Soo E, Thakur S, Qu Z, Jambhrunkar S, Popat A. 2015. Resveratrol nanoformulations: Challenges and opportunities. Int J Pharm 479:282-290.   DOI
30 Yangilar F. 2013. As a potentially functional food: Goats' milk and products. J Food Nutr Res 1:68-81.
31 Yuan Q, Shah J, Hein S, Misra RDK. 2010. Controlled and extended drug release behavior of chitosan-based nanoparticle carrier. Acta Biomater 6:1140-1148.   DOI
32 Zhang HL, Wu SH, Tao Y, Zang LQ, Su ZQ. 2010. Preparation and characterization of water-soluble chitosan nanoparticles as protein delivery system. J Nanomater 2010:1-5.
33 Zhang Y, Yang M, Portney NG, Cui D, Budak G, Ozbay E, Ozkan M, Ozkan CS. 2008. Zeta potential: A surface electrical characteristic to probe the interaction of nanoparticles with normal and cancer human breast epithelial cells. Biomed Microdevices 10:321-328.   DOI
34 Chen L, Remondetto GE, Subirade M. 2006. Food protein-based materials as nutraceutical delivery systems. Trends Food Sci Technol 17:272-283.   DOI
35 Bu L, Gan LC, Guo XQ, Chen FZ, Song Q, Zhao Q, Gou XJ, Hou SX, Yao Q. 2013. Trans-resveratrol loaded chitosan nanoparticles modified with biotin and avidin to target hepatic carcinoma. Int J Pharm 452:355-362.   DOI
36 Cao Y, Li J, Liu F, Li X, Jiang Q, Cheng S, Gu Y. 2016. Consideration of interaction between nanoparticles and food components for the safety assessment of nanoparticles following oral exposure: A review. Environ Toxicol Pharmacol 46:206-210.   DOI
37 Champagne CP, Fustier P. 2007. Microencapsulation for the improved delivery of bioactive compounds into foods. Curr Opin Biotechnol 18:184-190.   DOI
38 Desai P, Patlolla RR, Singh M. 2010. Interaction of nanoparticles and cell-penetrating peptides with skin for transdermal drug delivery. Mol Memb Biol 27:247-259.   DOI
39 Zhao L, Zhang S, Uluko H, Liu L, Lu J, Xue H, Kong F, Lv J. 2014. Effect of ultrasound pretreatment on rennet-induced coagulation properties of goat's milk. Food Chem 165:167-174.   DOI
40 Desai MP, Labhasetwar V, Amidon GL, Levy RJ. 1996. Gastrointestinal uptake of biodegradable microparticles: Effect of particle size. Pharm Res 13:1838-1845.   DOI
41 Du YZ, Wang L, Yuan H, Wei XH, Hu FQ. 2009. Preparation and characteristics of linoleic acid-grafted chitosan oligosaccharide micelles as a carrier for doxorubicin. Colloids Surf B Biointerfaces 69:257-263.   DOI
42 Brooke-Taylor S, Dwyer K, Woodford K, Kost N. 2017. Systematic review of the gastrointestinal effects of A1 compared with A2 ${\beta}$-casein. Adv Nutr 8:739-748.   DOI
43 Esmaili M, Ghaffari SM, Moosavi-Movahedi Z, Atri MS, Sharifizadeh A, Farhadi M, Tousefi R, Chobert JM, Haertle T, Moosavi-Movahedi AA. 2011. ${\beta}$-Casein-micelle as a nano vehicle for solubility enhancement of curcumin; food industry application. LWT-Food Sci Technol 44:2166-2172.   DOI
44 Ha HK, Jeon NE, Kim JW, Han KS, Yun SS, Lee MR, Lee WJ. 2016. Physicochemical characterization and potential prebiotic effect of whey protein isolate/inulin nano complex. Korean J Food Sci Anim Resour 36:267-274.   DOI
45 Zou P, Yang X, Wang J, Li Y, Yu H, Zhang Y, Liu G. 2016. Advances in characterisation and biological activities of chitosan and chitosan oligosaccharides. Food Chem 190:1174-1181.   DOI
46 Zu Y, Zhang Y, Wang W, Zhao X, Han X, Wang K, Ge Y. 2014. Preparation and in vitro/in vivo evaluation of resveratrolloaded carboxymethyl chitosan nanoparticles. Drug Deliv 23:971-981.   DOI
47 Estevinho BN, Rocha F, Santos L, Alves A. 2013. Microencapsulation with chitosan by spray drying for industry applications: A review. Trends Food Sci Technol 31:138-155.   DOI
48 Faizullin DA, Konnova TA, Haertle T, Zuev YF. 2013. Self-assembly and secondary structure of ${\beta}$-casein. Russ J Bioorg Chem 39:366-372.   DOI
49 George M, Abraham TE. 2006. Polyionic hydrocolloids for the intestinal delivery of protein drugs: Alginate and chitosan: A review. J Control Release 114:1-14.   DOI
50 Gokce EH, Korkmaz E, Dellera E, Sandri G, Bonferoni MC, Ozer O. 2012. Resveratrol-loaded solid lipid nanoparticles versus nanostructured lipid carriers: Evaluation of antioxidant potential for dermal applications. Int J Nanomed 7:1841-1850.
51 Ha HK, Kim JW, Lee MR, Jun WJ, Lee WJ. 2015. Cellular uptake and cytotoxicity of ${\beta}$-lactoglobulin nanoparticles: The effects of particle size and surface charge. Asian-Australas J Anim Sci 28:420-427.   DOI
52 Ha HK, Kim JW, Lee MR, Lee WJ. 2013. Formation and characterization of quercetin-loaded chitosan oligosaccharide/${\beta}$-lactoglobulin nanoparticle. Food Res Int 52:82-90.   DOI
53 Ha HK, Lee MR, Lee WJ. 2018a. Bioaccessibility of ${\beta}$-lactoglobulin nanoemulsions containing coenzyme $Q_{10}$: Impact of droplet size on the bioaccessibility of coenzyme $Q_{10}$. Korean J Food Sci Anim Resour 38:1294-1304.   DOI
54 Ha HK, Lee MR, Lee WJ. 2018b. Oxidative stability of DHA in ${\beta}$-lactoglobulin/oleic acid-modified chitosan oligosaccharide nanoparticles during storage in skim milk. LWT-Food Sci Technol 90:440-447.   DOI