Browse > Article
http://dx.doi.org/10.5851/kosfa.2018.e54

Optimization of 1D 1H Quantitative NMR (Nuclear Magnetic Resonance) Conditions for Polar Metabolites in Meat  

Kim, Hyun Cheol (Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University)
Ko, Yoon-Joo (National Center for Inter-University Research Facilities, Seoul National University)
Kim, Minsu (Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University)
Choe, Juhui (Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University)
Yong, Hae In (Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University)
Jo, Cheorun (Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute of Agriculture and Life Science, Seoul National University)
Publication Information
Food Science of Animal Resources / v.39, no.1, 2019 , pp. 1-12 More about this Journal
Abstract
The objective of this study was to establish an optimized 1D $^1H$ quantitative nuclear magnetic resonance (qNMR) analytical method for analyzing polar metabolites in meat. Three extraction solutions [0.6 M perchloric acid, 10 mM phosphate buffer, water/methanol (1:1)], three reconstitution buffers [20 mM 3-morpholinopropane-1-sulfonic acid, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, phosphate buffer], and two pulse programs (zg30, noesypr1d) were evaluated. Extraction with 0.6 M perchloric acid and 20 mM phosphate resulted in a stable baseline and no additional overlap for quantifying polar metabolites in chicken breast. In qNMR analysis, zg30 pulse program (without water-suppression) showed smaller relative standard deviation (RSD) and faster running time than noesypr1d (water-suppression). High-performance liquid chromatography was compared with qNMR analyses to validate accuracy. The zg30 pulse program showed good accuracy and lower RSD. The optimized qNMR method was able to apply for beef and pork samples. Thus, an optimized 1D $^1H$ qNMR method for meat metabolomics was established.
Keywords
qNMR; extraction solution; reconstitution buffer; polar metabolites; meat;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Gallo V, Intini N, Mastrorilli P, Latronico M, Scapicchio P, Triggiani M, Bevilacqua V, Fanizzi P, Acquotti D, Airoldi C, Arnesano F, Assfalg M, Benevelli F, Bertelli D, Cagliani LR, Casadei L, Marincola FC, Colafemmina G, Consonni R, Cosentino C, Davalli S, De Pascali SA, D'Aiuto V, Faccini A, Gobetto R, Lamanna R, Liguori F, Longobardi F, Mallamace D, Mazzei P, Menegazzo I, Milone S, Mucci A, Napoli C, Pertinhez T, Rizzuti A, Rocchigiani L, Schievano E, Sciubba F, Sobolev A, Tenori L, Valerio M. 2015. Performance assessment in fingerprinting and multi component quantitative NMR analyses. Anal Chem 87:6709-6717.   DOI
2 Govindaraju V, Young K, Maudsley AA. 2000. Proton NMR chemical shifts and coupling constants for brain metabolites. NMR Biomed 13:129-153.   DOI
3 Graham SF, Kennedy T, Chevallier OP, Gordon A, Farmer L, Elliott C, Moss BW. 2010. The application of NMR to study changes in polar metabolite concentrations in beef longissimus dorsi stored for different periods post mortem. Metabolomics 6:395-404.   DOI
4 Henderson JW, Ricker RD, Bidlingmeyer BA, Woodward C. 2000. Rapid, accurate, sensitive, and reproducible HPLC analysis of amino acids. Available from https://www.agilent.com/cs/library/chromatograms/59801193.pdf. Accessed at Sep 12, 2018.
5 Jayasena DD, Ahn DU, Nam KC, Jo C. 2013. Factors affecting cooked chicken meat flavour: A review. Worlds Poult Sci J 69:515-526.   DOI
6 Jayasena DD, Jung S, Kim HJ, Yong HI, Nam KC, Jo C. 2015. Taste-active compound levels in Korean native chicken meat: The effects of bird age and the cooking process. Poult Sci 94:1964-1972.   DOI
7 Jung S, Bae YS, Kim HJ, Jayasena DD, Lee JH, Park HB, Heo KN, Jo C. 2013. Carnosine, anserine, creatine, and inosine 5′-monophosphate contents in breast and thigh meats from 5 lines of Korean native chicken. Poult Sci 92:3275-3282.   DOI
8 Lin CY, Wu H, Tjeerdema RS, Viant MR. 2007. Evaluation of metabolite extraction strategies from tissue samples using NMR metabolomics. Metabolomics 3:55-67.   DOI
9 Krssak M, Mlynarik V, Meyerspeer M, Moser E, Roden M. 2004. $^1H$ NMR relaxation times of skeletal muscle metabolites at 3 T. Magn Reson Mater Phys Biol Med 16:155-159.   DOI
10 Lee HJ, Choe J, Kim KT, Oh J, Lee DG, Kwon KM, Choi YI, Jo C. 2017. Analysis of low-marbled Hanwoo cow meat aged with different dry-aging methods. Asian Australas J Anim Sci 30:1733-1738.   DOI
11 Martineau E, Tea I, Akoka S, Giraudeau P. 2012. Absolute quantification of metabolites in breast cancer cell extracts by quantitative 2D $^1H$ INADEQUATE NMR. NMR Biomed 25:985-992.   DOI
12 Mckay RT. 2011. How the 1D-NOESY suppresses solvent signal in metabolomics NMR spectroscopy: An examination of the pulse sequence components and evolution. Concepts Mag Reson A 38:197-220.   DOI
13 Nowick JS, Khakshoor O, Hashemzadeh M, Brower JO. 2003. DSA: A new internal standard for NMR studies in aqueous solution. Org Lett 5:3511-3513.   DOI
14 Aristoy MC, Toldra F. 1991. Deproteinization techniques for HPLC amino acid analysis in fresh pork muscle and dry-cured ham. J Agr Food Chem 39:1792-1795.   DOI
15 Piao MY, Lee HJ, Yong HI, Kim HJ, Jo C, Wiryawan KG, Baik M. 2019. Comparison of reducing sugar content, sensory traits, and fatty acids and volatile compound profiles of the longissimus thoracis among Korean cattle, Holsteins, and Angus steer. Asian Australas J Anim Sci 32:126-136.   DOI
16 Ramakrishnan V, Luthria DL. 2017. Recent applications of NMR in food and dietary studies. J Sci Food Agric 97:33-42.   DOI
17 Jung Y, Lee J, Kwon J, Lee KS, Ryu DH, Hwang GS. 2010. Discrimination of the geographical origin of beef by $^1H$ NMR-based metabolomics. J Agr Food Chem 58:10458-10466.   DOI
18 Aliani M, Farmer LJ, Kennedy JT, Moss BW, Gordon A. 2013. Post-slaughter changes in ATP metabolites, reducing and phosphorylated sugars in chicken meat. Meat Sci 94:55-62.   DOI
19 Araníbar N, Ott KH, Roongta V, Mueller L. 2006. Metabolomic analysis using optimized NMR and statistical methods. Anal Biochem 355:62-70.   DOI
20 Bharti SK, Roy R. 2012. Quantitative $^1H$ NMR spectroscopy. Trends Analyt Chem 35:5-26.   DOI
21 Choe JH, Nam KC, Jung S, Kim BN, Yun HJ, Jo C. 2010. Differences in the quality characteristics between commercial Korean native chickens and broilers. Korean J Food Sci Anim Resour 30:13-19.   DOI
22 Schreurs FJG. 2000. Post-mortem changes in chicken muscle. Worlds Poult Sci J 56:319-346.   DOI
23 Romisch-Margl W, Prehn C, Bogumil R, Rohring C, Suhre K, Adamski J. 2012. Procedure for tissue sample preparation and metabolite extraction for high-throughput targeted metabolomics. Metabolomics 8:133-142.   DOI
24 Saito T, Nakaie S, Kinoshita M, Ihara T, Kinugasa S, Nomura A, Maeda T. 2004. Practical guide for accurate quantitative solution state NMR analysis. Metrologia 41:213-218.   DOI
25 Saranadasa H. 2000. RSD requirement for different sample size for blend sampling. Drug Dev Ind Pharm 26:1213-1216.   DOI
26 Schwarz EL, Roberts WL, Pasquali M. 2005. Analysis of plasma amino acids by HPLC with photodiode array and fluorescence detection. Clin Chim Acta 354:83-90.   DOI
27 Siciliano C, Belsito E, De Marco R, Di Gioia ML, Leggio A, Liguori A. 2013. Quantitative determination of fatty acid chain composition in pork meat products by high resolution $^1H$ NMR spectroscopy. Food Chem 136:546-554.   DOI
28 Cornet M, Bousset J. 1999. Free amino acids and dipeptides in porcine muscles: Differences between 'red' and 'white' muscles. Meat Sci 51:215-219.   DOI
29 Dietmair S, Timmins NE, Gray PP, Nielsen LK, Kromer JO. 2010. Towards quantitative metabolomics of mammalian cells: Development of a metabolite extraction protocol. Anal Biochem 404:155-164.   DOI
30 Feidt C, Petit A, Bruas-Reignier F, Brun-Bellut J. 1996. Release of free amino-acids during ageing in bovine meat. Meat Sci 44:19-25.   DOI
31 Simmler C, Napolitano JG, McAlpine JB, Chen SN, Pauli GF. 2014. Universal quantitative NMR analysis of complex natural samples. Curr Opin Biotechnol 25:51-59.   DOI
32 Warris PD. 1979. The extraction of haem pigments from fresh meat. Int J Food Sci Technol 14:75-80.   DOI
33 Wishart DS. 2008. Quantitative metabolomics using NMR. Trends Analyt Chem 27:228-237.   DOI
34 Xiao C, Hao F, Qin X, Wang Y, Tang H. 2009. An optimized buffer system for NMR-based urinary metabolomics with effective pH control, chemical shift consistency and dilution minimization. Analyst 134:916-925.   DOI
35 Zhu LG, Bidner B, Brewer MS. 2001. Postmortem pH, muscle, and refrigerated storage effects on ability of vacuum-packaged pork to bloom. J Food Sci 66:1230-1235.   DOI