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http://dx.doi.org/10.5851/kosfa.2013.33.2.239

First Report on Isolation of Salmonella Enteritidis from Eggs at Grocery Stores in Korea  

Kim, Young Jo (Livestock Product Standard Division, Food Microbiology Division, and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety)
Song, Bo Ra (Livestock Product Standard Division, Food Microbiology Division, and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety)
Lim, Jong Su (College of Veterinary Medicine, Konkuk University)
Heo, Eun Jeong (Livestock Product Standard Division, Food Microbiology Division, and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety)
Park, Hyun Jung (Livestock Product Standard Division, Food Microbiology Division, and Agro-Livestock & Fishery Products Policy Division, Ministry of Food and Drug Safety)
Wee, Sung Hwan (Veterinary Pharmaceutical Management Division, Animal and Plant Quarantine Agency)
Oh, Soon Min (Quarantine Policy Division, Ministry of Agriculture, Food and Rural Affairs)
Moon, Jin San (Veterinary Pharmaceutical Management Division, Animal and Plant Quarantine Agency)
Publication Information
Food Science of Animal Resources / v.33, no.2, 2013 , pp. 239-243 More about this Journal
Abstract
Salmonella Enteritidis is responsible for causing foodborne diseases upon consumption of egg products. While cases of S. Enteritidis isolation from eggs have been reported in other countries, no such cases have previously been reported in Korea. In this study, we report the first isolation and identification of S. Enteritidis from domestically distributed eggs in Korea. Eggs were collected from eight countrywide grocery stores during different seasons between 2011 and 2012. Egg contents and washing solution of egg shells were incubated in buffered peptone water, and the enriched broth was further enriched in tetrathionate broth and Rappaport-Vassiliadis. The secondary enriched broth was streaked on xylose lysine desoxycholate agar. The suspected colonies were confirmed to S. Enteritidis by a biochemical test, serotyping, and PCR test. Genetic relatedness among the isolates was analyzed using Diversilab Salmonella kit. Three strains of S. Enteritidis were isolated from egg contents and egg shells collected from grocery stores of the Eumseong-city in the fall of 2011. All three stains showed resistance to chloramphenicol, streptomycin, nalidixic acid, and ampicillin by the disk diffusion method. In addition, the isolates showed more than 99% DNA homology, indicating that they were presumably identical strains. Therefore, there is a requirement to monitor and control against S. Enteritidis from eggs in Korea.
Keywords
egg; Salmonella Enteritidis; isolation; antibiotic resistance;
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Times Cited By KSCI : 5  (Citation Analysis)
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1 Duguid, J. P. and North, R. A. E. (1991) Egg and Salmonella food poisoning: an evaluation. J. Med. Microbiol. 34, 65-67.   DOI
2 Ewing, W. H. (1986) Antigenic Schema for Salmonella. In: Edwards and Ewing's Identification of Enterobacteriaceae, 4th Ed. Elsevier Science Publishing Co. Inc., New York, pp. 247-318.
3 FDA (Food and Drug Administration). (2000) Prevention of Salmonella Enteritidis in Shell Eggs During Production, Storage, and Transportation (FDA-2000-N-0190).
4 Henzler, D. J., Kradel, D. C., and Sischo, W. M. (1998) Management and environmental risk factors for Salmonella enteritidis contamination of eggs. Am. J. Vet. Res. 59, 824-829.
5 Henzler, D. J., Ebel, E., and Sanders, J. (1994) Salmonella Enteritidis in eggs from commercial chicken layer flocks implicated in human outbreak. Avian Dis. 38, 37-43.   DOI   ScienceOn
6 Cho, J. K., Kang, M. S., and Kim, K. S. (2011) Serotypes, antimicrobial resistance of Salmonella spp. and plasmid profiles, phage types, PFGE of S. Enteritidis and S. Typhimurium isolated from ducks in Daegu-Gyeongbuk province. Korean J. Vet. Serv. 34, 217-226.   과학기술학회마을   DOI   ScienceOn
7 Choi, W. Z., Jung, J. H., Won, H. K., Kang, Z. W., and Hahn, T. W. (2008) Serotypes and genotypes of Salmonella isolates from slaughtered pigs. Korean J. Vet. Serv. 31, 1-16.   과학기술학회마을
8 Chun, M. S. and Hong, S. H. (2009) Identification of microorganisms from eggs in hypermarket in the Northern Gyeonggi area. Korean J. Food Nutr. 22, 394-401.   과학기술학회마을
9 CLSI (Clinical Laboratory and Standards Institute). (2012) Performance standards for antimicrobial susceptibility testing: Twenty-second informational supplement. M100-S22.
10 Codex (1976) Code of Hygienic Practice for Eggs and Egg products (CAC/RCP 12-1976).
11 Cowden, J. M., Chisholm, D., O'Mahony, M., Lynch, D., Mawer, S. L., Spain, G. E., Ward, L., and Rowe, B. (1989) Two outbreaks of Salmonella Enteritidis PT4 infection associated with the consumption of fresh shell egg products. Epidemiol. Infect. 103, 47-52.   DOI
12 Coyle, E. F., Ribeiro, C. D., Howard, A. J., Palmer, S. R., Jones, H. I., Ward, L., and Rowe, B. (1988) Salmonella Enteritidis PT4 infection: Association with hens' eggs. Lancet 2, 1295-1297.
13 Wilson, I. G., Heaney, J. C., and Powell, G. G. (1998) Salmonella in raw shell eggs in Northern Ireland. Commun. Dis. Public Health. 1, 156-160.
14 Soumet, C., Ermel, G., Rose, N., Drouin, P., Salvat, G., and Colin, P. (1999) Evaluation of a Multiplex PCR assay for simultaneous identification of Salmonella spp., Salmonella Enteritidis and Salmonella typhimurium from environmental swabs of poultry house. Lett. Appl. Microbiol. 28, 113-117.   DOI   ScienceOn
15 St. Louis, M. E., Morse, D. L., Potter, M. E., DeMelfi, T. M., Guzewich, J. J., Tauxe, R. V., and Blake, P. A. (1988) The emergence of grade A eggs as a major source of Salmonella Enteritidis infections. J. Am. Med. Assoc. 259, 2103-2107.   DOI   ScienceOn
16 Watkins B. A. (1995) The nutritive value of the egg. In: Egg science and technology Stadelman, W. J., and Cotterill, O. J. The Haworth press. 4th, pp. 177-194.
17 Woo, Y. K. (2005) Microbial hygienic status of poultry meats and eggs collected at the public markets in Seoul and Kyunggi regions in 1996. Korean J. Microbiol. 41, 38-46.
18 Altin, T., Bizena, B., Kapllan, S., and Elvira, B. (1999) Occurrence of Salmonella spp. in imported eggs into Albania. Int. J. Food Microbiol. 49, 169-171.   DOI   ScienceOn
19 Amedo, A., Bellido, J. B., Pac, M. R., Criado, J., Usera, M. A., Mesanza, I., Gonzales, F., Perez, R., and Cortes, J. M. (1998) Epidemic outbreaks of salmonellosis caused by eating eggs. Enferm. Infecc. Microbiol. Clin. 16, 408-412.
20 Bacteriological Analytical Manual (BAM, U.S. food and drug adminstration).
21 Baker, R. C., Golf, J. P., and Timoney, J. P. (1980) Prevalence of Salmonella on eggs from poultry farms in New York State. Poult. Sci. 59, 289-292.   DOI   ScienceOn
22 Lee, H. W., Hong, C. H., and Jung, B. Y. (2007) Characteristics of Salmonella spp. isolated from poultry carcasses. Korean J. Vet. Serv. 30, 339-351.   과학기술학회마을
23 Humphrey, T. J., Greenwood, M., Gilbert, R. J., Rowe, B., Chapman, P. A. (1989) The survival of Salmonellas in shell eggs cooked under simulated domestic conditions. Epiderm. Infect. 103, 35-45.   DOI
24 Jang, K. I., Park, J. H., and Kim, K. Y. (1999) Studies on Salmonella Enteritidis contamination in chicken egg using confocal scanning laser microscopy. Korean J. Food Sci. Technol. 31, 771-777.   과학기술학회마을
25 Jones, F. T., Rives, D. V., and Carey, J. B. (1995) Salmonella contamination in commercial eggs and an egg production facility. Poultry Sci. 74, 753-757.   DOI   ScienceOn
26 Lee, S. M., Kim, K. H., Lee, J. G., Park, E. J., Lee, S. W., and Hong, C. H. (2002) Hygienic quality of eggs in the department food stores in the Incheon Metropolitan area. J. Fd. Hyg. Safety 17, 129-136.   과학기술학회마을
27 Rowe, B. (1989) Salmonella Enteritidis: The program nationally and internationally. In: proc. Xth. Int. Symp. World Assoc. Vet Food Hyg., Stockholm. 326.
28 Lin, F. Y., Morris, J. R., Trump, D., Tilghman, D., Wood, P. K., Jackman, N., Israel, E., and Libonati, J. P. (1988) Investigation of an outbreak of Salmonella Enteritidis gastroenteritis associated with consumption of eggs in a restaurant chain in Maryland. Am. J. Epidemiol. 128, 839-844.
29 MAFRA (Ministry of Agriculture, Food and Rural Affairs). (2009) Microbiological and chemical residual inspection on shell egg (MiFAFF Notice 2009-169).
30 QIA (Animal and Plant Quarantine Agency). (2012) Standards for processing and ingredients specifications of livestock products (QIA notice 2012-162).