Browse > Article
http://dx.doi.org/10.5851/kosfa.2011.31.2.290

Development of Probiotic Dairy Product for the Normalization of Microbial Flora in Korean Infants  

Kim, Min-Kyung (National Institute of Animal Science, RDA)
Choi, A-Ri (National Institute of Animal Science, RDA)
Han, Gi-Sung (National Institute of Animal Science, RDA)
Jeong, Seok-Geun (National Institute of Animal Science, RDA)
Chae, Hyun-Seok (National Institute of Animal Science, RDA)
Jang, Ae-Ra (National Institute of Animal Science, RDA)
Seol, Kuk-Hwan (National Institute of Animal Science, RDA)
Oh, Mi-Hwa (National Institute of Animal Science, RDA)
Kim, Dong-Hun (National Institute of Animal Science, RDA)
Ham, Jun-Sang (National Institute of Animal Science, RDA)
Publication Information
Food Science of Animal Resources / v.31, no.2, 2011 , pp. 290-295 More about this Journal
Abstract
This study was conducted to develop an effective probiotic dairy product to normalize the microbial flora in Korean infants. A total of 2,200 colonies were isolated from 25 Korean neonates, and 16S rRNA of 348 isolates was analyzed. Approximately 40% of the lactic-acid producing bacterial isolates were Enterococcus faecalis, and 34.2% of them were strains similar to XR7 in the GenBank database. The fastest growing strain in MRS broth was registered as 91532 by the KACC. The selected strain was freeze-dried and utilized to ferment a milk-containing rice soup, tarakjuk. Microbiological, physico-chemical, and sensory characteristics of the fermented tarakjuk were compared with fermented milk and tarakjuk. E. faecalis KACC 91532 increased from $6.14{\pm}0.19$ to $7.36{\pm}0.13$ Log CFU/mL, and can be useful as a probiotic, as described in the Standards for Functional Health Foods.
Keywords
probiotics; Enterococcus faecalis; fermented tarakjuk;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
1 Lee, S. H., Yang, E. H., Kwon, H. S., Kang, J. H., and Kang, B. H. (2008) Potential probiotic properties of Lactobacillus johnsonii IDCC 9203 isolated from infant feces. Korean J. Microbiol. Biotechnol. 36, 121-127.   과학기술학회마을
2 Mainville, I., Y. Arcand, and E. R. Farnworth. (2005) A dynamic model that simulates the human upper gastrointestinal tract for the study of probiotics. Int. J. Food Microbiol. 99, 287-296.   DOI   ScienceOn
3 McKinley, M. C. (2005) Effects of probiotics on biotechnological characteristics of yogurt. Int. J. Dairy Technol. 58, 1-12.   DOI   ScienceOn
4 Mishra, V. and Prasad, D. N. (2005) Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics. Int. J. Food. Microbiol. 103, 109-115.   DOI   ScienceOn
5 Moon, B. Y., Lee, S. K., and Park, J. H. (2006) Antibiotic resistant characteristics of Bifidobacterium from Korean institute origin and commercial yoghurts. Korean J. Food Sci. Technol. 38, 313-316.
6 Park, J. G., Yun, S. Y., Oh, S, Shin, J. G., and Baek, Y. J. (2003) Probiotic characteristics of Lactobacillus acidophilus KY 1909 isolated from Korean breast-fed infant. Korean J. Food Sci. Technol. 35, 1244-1247.
7 Penders, J., Thijs, C., Vink, C., Stelma, F. F., Snijders, B., Kummeling, I, van den Brand, P. A., and Stobberingh, E. E. (2009) Factors influencing the composition of the intestinal microbiota in early infancy. Pediatrics 118, 511-521.
8 Pinto, M. G. V., Franz, C. M. A. P., Schillinger, U., and Holzapfel, W. H. (2006) Lactobacillus sp. with in vitro probiotic properties from human faeces and traditional fermented products. Int. J. Food Microbiol. 109, 205-214.   DOI   ScienceOn
9 Schillinger, U., Guigas, C., and Holzapfel, W. H. (2005) In vitro adherence and other properties of lactobacilli used in probiotic yoghurt-like products. Int. Dairy J. 15, 1289-1297.   DOI   ScienceOn
10 Fanaro, S., Chierici, R., Guerrini, P., and Vigi, V. (2003) Intestinal microflora in early infancy: composition and development. Acta Paediatr. Suppl. 91, 48-55.
11 Kang, D. G., Kang, S. P., Chang, D. H., Kim, S. H., and Yoon, S. S. (2001) Isolation and characterization of Lactobacillus strains isolated from Korean feces. Korean J. Food Sci. Technol. 33, 567-573.   과학기술학회마을
12 Klevin, G. (2003) Taxonomy, ecology and antibiotic resistance of enterococci from food and gastro-intestinal tract. Int. J. Food Microbiol. 88, 123-131.   DOI   ScienceOn
13 KFDA (Korea Food and Drug Administration) (2008) Standard for Functional Health Foods. KFDA Notice 2008-64.
14 KDIA (Korea Dairy Industries Association) (2008) Domestic Statistics. Available from: http://www.koreadia.or.kr/statistics/sub04.html. http://www.koreadia. or.kr/statistics/sub11.html Accessed May. 7. 2010.
15 Lee, S. G., Han, G. S., Jeong, S. G., Oh, M. H., Jang, A., Kim, D. H., Bae, I. H., and Ham, J. S. (2010) A study on the sensory characteristics of yogurt and antimicrobial activity of Lactobacillus plantarum LHC52 isolated from Kimchi. Korean J. Food Sci. Ani. Resour. 30, 328-335.   DOI   ScienceOn