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http://dx.doi.org/10.5851/kosfa.2010.30.1.87

Development of Polyclonal Antibodies to Abdominal and Subcutaneous Adipocyte for Producing Fat-reduced High Quality Pork  

Choi, Chang-Weon (Nutrition and Physiology Team, National Institute of Animal Science, RDA)
Baek, Kyung-Hoon (School of Biotechnology, Yeungnam University)
Choi, Chang-Bon (School of Biotechnology, Yeungnam University)
Oh, Young-Kyoon (Nutrition and Physiology Team, National Institute of Animal Science, RDA)
Hong, Seong-Koo (Nutrition and Physiology Team, National Institute of Animal Science, RDA)
Publication Information
Food Science of Animal Resources / v.30, no.1, 2010 , pp. 87-94 More about this Journal
Abstract
The aim of the present study was to develop polyclonal antibodies to regional inedible adipocytes of pigs and investigate the effect of these antibodies on adipocytes in vitro. As antigens, abdominal and subcutaneous adipocyte PMPs from pigs were injected into sheep 3 times per 3 wk intervals for passive immunization, and non-immunized serum, antisera against abodominal (AAb) or subcutaneous adipocyte PMPs (SAb) were collected before and after the injections. Titers of the antisera obtained from sheep and their cross-reactivities with the heart, kidney, liver, lung, muscle, and spleen of pig were determined by ELISA. Isolation and culture of abdominal and subcutaneous adipocytes from pigs were performed to analyze LDH concentration. At a 1:1,000 dilution, little antibody reactivity was observed for non-immunized serum whereas both AAb and SAb had relatively strong reactivity up to a dilution of 1:16,000. These findings may indicate that strong antibodies against adipocyte PMPs can be developed using an immunological approach. Extremely low reactivity of AAb and SAb was detected with the PMPs of the organs. Both antisera most strongly reacted with each adipocyte PMPs and showed statistically (p<0.05) higher cross-reactivities compared with the non-immunized serum. In conclusion, these results may indicate that the present polyclonal antibodies against regional inedible adipocyte PMPs are well developed and are safe against cross-reactivities with the organs of pigs. Further studies on the in vivo nutritional safety and fat reduction of these antibodies in pigs will be required fat-reduced high quality pork production.
Keywords
high quality pork; antibody; subcutaneous adipocyte; cross-reactivity;
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