Browse > Article
http://dx.doi.org/10.5851/kosfa.2009.29.5.647

Rapid Identification of Cow and Goat Milk in Milk Products Using a Duplex PCR Technique  

Lee, Seung-Bae (Division of Animal Resources and Life Science, Sangji University)
Choi, Suk-Ho (Division of Animal Resources and Life Science, Sangji University)
Publication Information
Food Science of Animal Resources / v.29, no.5, 2009 , pp. 647-652 More about this Journal
Abstract
A duplex PCR technique was applied for specific identification of cow and goat milk in milk products by using primers targeting the mitochondrial 12S rRNA gene. Duplex PCR using primers specific for cow and goat generated specific fragments of 223bp and 326bp from cow and goat milk DNA, respectively. Duplex PCR was applied to 15 milk products purchased from the market to verify label statements. The labeling statements of four market milk products, three yoghurt products, and one whole milk powder product were confirmed in the duplex PCR. The labeling statements of five of seven infant milk powder products were also confirmed by duplex PCR but the other two products were shown to be contaminated with either cow or goat milk. The proposed duplex PCR provides a rapid and sensitive approach to detection of as little as 0.1% cow milk in goat milk and one-step detection of cow or goat milk in milk products.
Keywords
duplex PCR; milk products; species identification; goat milk; cow milk;
Citations & Related Records

Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 De Noni, I., Tirelli, A., and Masotti. F. (1996) Detection of cows' milk in non-bovine cheese by HPLC of whey protein: Application to goat milk cheese. Sci. Tec. Latt. Casear. 47, 7-17
2 EC Regulation No. 1081/96 of the commission June 14 (1996) Reference method for detection of cow's milk and cow's milk casein in cheese made from ewes, goats and buffalo milk or mixtures of ewes, goats and buffalo milk. Official J. Euro. Commission L142, 15-25
3 Koh, M. C., Lim, C. H., Chua, S. B., Chew, S. T., and Phang, S. T. W. (1998) Random amplified polymorphic DNA (RAPD) fingerprints for identification of red meat animal species. Meat Sci. 48, 275-285   DOI   ScienceOn
4 Rodríguez, M. A., García, T., González, I., Asensio, L., Mayoral, B. L., Löpez-Calleja, I., Hernández, P. E., and Martín. R. (2003) Identification of goose, mule duck, chicken, turkey, and swine in foie gras by species-specific polymerase chain reaction. J. Agr. Food Chem. 51, 1524-1529   DOI   ScienceOn
5 Unseld, M., Beyermann, D., Brabdt, P., and Hiesel, R. (1995) Identification of the species origin of highly processed meat products by mitochondrial DNA sequences. PCR Meth. Appl. 4, 241-243   DOI
6 Wal, J. M. (2004) Bovine milk allergenicity. Ann Allergy Asthma Immun. 93, 2-11   DOI   ScienceOn
7 Meyer, R., Hofelein, C., Luthy, J., and Candrian, U. (1995) Polymerase chain reaction-restriction fragment length polymorphism analysis: a sample method for species identification in food. J. AOAC Int. 78, 1542-1551   PUBMED
8 Sampson, H. A. (2003) Food allergy. J. Allergy Clin. Immun. 111, 540-547   DOI   ScienceOn
9 Addeo, F., Moio, L., Chianese, L., Stingo, C., Resmini, P., Berner, I., Krause, I., Di Luccia, A., and Bocca, A. (1990) Use of plasmin to increase the sensitivity of the detection of bovine milk in ovine and/or caprine cheese by gel isoelectric focusing of $\gamma_2$-caseins. Milchwissenschaft 45, 708-711
10 Branciari, R., Nijman, I. J., Plas, M. E., Di Antonio, E., and Lenstra. J. A. (2000) Species origin of milk in Italian Mozzarella and Greek Feta cheese. J. Food Prot. 63, 408-411   DOI   PUBMED
11 Kotowiez, M., Adamczyk, E., and Bania, J. (2007) Application of a duplex-PCR for detection of cows' milk in goats' milk. Ann. Agr. Env. Med. 14, 215-218
12 Bottero, M. T., Civera, T., Nucera, D., Rosati, S., Sacchi, P., and Turi, R. M. (2003) A multiplex polymerase chain reaction for the identification of cows', goats' and sheeps' milk in dairy products. Int. Dairy J. 13, 277-282   DOI   ScienceOn
13 Lopez-Calleja, I., González, I., Fajardo, V., Rodriguez, M. A., Hernández, P. E., García, T., and Martin, R. (2004) Rapid detection of cows' milk in sheeps' and goats' milk by a species- specific polymerase chain reaction technique. J. Dairy Sci. 87, 2839-2845   DOI   ScienceOn
14 Anguita, G., Martin, R., Garcia, T.. Morales, P., Haza, A. I., Gonzalez, I., Sanz, B., and Hernandez, P. E. (1996) Immunostick ELISA for detection of cow's milk in ewe's milk and cheese using a monoclonal antibody against bovine $\beta$-casein. J. Food Prot. 59, 436-437   DOI
15 Haza, A. I., Morales, P., Martin, R., Garcia, T., Aguita, G., Gonzalez, I., Sanz, B., and Hernandez, P. E. (1997) Use of a monoclonal antibody and two enzyme-linked immunosorbent assay formats for detection and quantification of the substitution of caprine milk for ovine milk. J. Food Prot. 60, 973-977   DOI
16 Mafra, I., Ferreira, I. M., Faria, M. A., and Oliveira, B. P. (2004) A novel approach to the quantification of bovine milk in ovine cheese using a duplex polymerase chain reaction method. J. Agr. Food Chem. 52, 4943-4947   DOI   ScienceOn
17 Dalmasso, A., Fontanella, E., Piatti, P., civera, T., Rosati, S., and Bottero, M. T. (2004) A multiplex PCR assay for the identification of animal species in feedstuffs. Mol. Cell. Probes 18, 81-87   DOI   ScienceOn
18 Molina, E., Martín-Álvarez, P. J., and Ramos, M. (1999) Analysis of cows', ewes' and goats' milk mixtures by capillary electrophoresis: Quantification by multivariate regression analysis. Int. Dairy J. 9, 99-105   DOI   ScienceOn
19 Chikuni, K., Tabata, T., Kosugiyama, M., Monma, M., and Saito, M. (1994) Polymerase chain reaction assay for detection of sheep and goat meats. Meat Sci. 37, 337-345   DOI   ScienceOn
20 Rea, S., Chikuni, K., Branciari, R., Sangamayya, R. S., Ranucci, A., and Avellini, P. (2001) Use of duplex polymerase chain reaction (duplex-PCR) technique to identify bovine and water buffalo milk used in making mozzarella cheese. J. Dairy Res. 68, 689-698   DOI   ScienceOn
21 Maudet, C. and Taberlet, P. (2001) Detection of cows' milk in goats' cheeses inferred from mitochondrial DNA polymorphism. J. Dairy Res. 68, 229-235   DOI   ScienceOn
22 Amills, M., Francino, O., Jansa, M., and Sánchez, A. (1997) Isolation of genomic DNA from milk samples by using Chelex resin. J. Dairy Res. 64, 231-238   DOI   ScienceOn
23 Chianese, L., Laezza, P., Smaldone, L. A., Stingo, C., Del Giovine, L., and Addeo, F. (1990) Evaluation of bovine milk in the buffalo Mozzarella cheese by two-dimensional electrophoresis. Sci. Tec. Latt. Casear. 41, 315-326
24 Haenlein, G. F. W. (1995) Status and prospects of the dairy goat industry in the united states. J. Anim. Sci. 74, 1173-1181
25 Kocher, T. D., Thomas, W. K., Mayer, A., Edwards, S. V., Paabo, S., Villablanca, F. X., and Wilson A. C. (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc. Natl. Acad. Sci. 86, 6196-6200   DOI   ScienceOn
26 Calvo, J. H., Osta, R., and Zaragoza, P. (2002) Species-specific amplification for detection of bovine, ovine and caprine cheese. Milchwissenschaft 57, 4-446