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http://dx.doi.org/10.5851/kosfa.2008.28.4.457

Purification and Characterization of Lacticin NK34 Produced by Lactococcus lactis NK34 against Bovine Mastitis  

Lee, Na-Kyoung (Division of Animal Life Science, Konkuk University)
Park, Yeo-Lang (Division of Animal Life Science, Konkuk University)
Kim, Hyoun-Wook (Division of Animal Life Science, Konkuk University)
Park, Yong-Ho (College of Veterinary Medicine, Seoul University)
Rhim, Seong-Lyul (Department of Biomedical Science, Hallym University)
Kim, Jong-Man (National Veterinary Research & Quarantine Service)
Kim, Jae-Myung (National Veterinary Research & Quarantine Service)
Nam, Hyang-Mi (National Veterinary Research & Quarantine Service)
Jung, Suk-Chan (National Veterinary Research & Quarantine Service)
Paik, Hyun-Dong (Division of Animal Life Science, Konkuk University)
Publication Information
Food Science of Animal Resources / v.28, no.4, 2008 , pp. 457-462 More about this Journal
Abstract
Lactococcus lactis NK34, isolated from jeotgal (Korean traditional fermented fish), produces bacteriocin against bovine mastitis pathogens such as Staphylococcus aureus 7, S. aureus 8, Staphylococcus chromogenes 10, S. chromogenes 19, Staphylococcus hominis 9, Streptococcus uberis E290, Enterococcus faecium E372, Streptococcus agalactiae ATCC 13813, Pseudonocardia autotrophia KCTC 9455, and Staphylococcus simulans 78. Lacticin NK34 was inactivated by protease XIV but not by protease IX, protease XIII, proteinase K, $\acute{a}$-chymotrypsin, trypsin, and pepsin. Also, lacticin NK34 was stable over a pH range of 2 to 9 for 4 hr and withstood exposure to temperatures of 30-$100^{\circ}C$ for 30 min. Lacticin NK34 showed bactericidal effects against S. simulans 78. This bacteriocin was purified using ammonium sulfate precipitation, ion exchange chromatography, ultrafiltration, and hydrophobic chromatography. Tricin-SDS-PAGE of purified bacteriocin gave the same molecular weight (3.5 kDa) as nisin. The gene encoding this bacteriocin was amplified by PCR using nisin gene-specific primers. It showed similar sequences to this nisin Z gene. These results indicate that lacticin NK34 is a nisin-like bacteriocin, and could be used as an antimicrobial alternative for livestock.
Keywords
Lactococcus lactis NK34; lacticin NK34; bovine mastitis; purification; characterization; nisin;
Citations & Related Records
Times Cited By KSCI : 6  (Citation Analysis)
Times Cited By Web Of Science : 2  (Related Records In Web of Science)
Times Cited By SCOPUS : 3
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