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Effects of IGF-I Rich Fraction from Bovine Colostral Whey on Immune Activity of Mouse In Vitro  

Hwang Hyung-A (Department of Food Science & Biotechnology, Sunggkyunkwan University)
Yang Hee-Jin (Department of Food Science & Biotechnology, Sunggkyunkwan University)
Lee Soo-Won (Department of Food Science & Biotechnology, Sunggkyunkwan University)
Publication Information
Food Science of Animal Resources / v.26, no.1, 2006 , pp. 113-120 More about this Journal
Abstract
Insulin-like growth factor-I(IGF-I) rich fraction, which was obtained molecules ranged between 30 and 1 kDa, was fractionated by ultrafiltration from bovine colostral whey. IGF-I rich fraction was confirmed by SDS-PACE and western blotting and then the quantity of IGF-I was measured by sandwich ELISA. ICF-I concentration in IGF-I rich fraction was 10 ng/mg proteins. IGF-I rich fraction, standard IGF-I and colostral whey weie treated to murine peritoneal macrophages. And then we experimented that effect of immune activity on macrophage and splenocyte. As a result, in group treated with IGF-I rich fraction $1{mu}g/mL$, production of interleukin-6 and nitric oxide were 9.85 ng and $17.17{\mu}M$ and production of phagocytosis, tumor necrosis factor-${\alpha}\;and\;H_{2}O_{2}$ were 78.3, 34.5 and 6% compared to the control group. In splenocyte immune response, B cell and T cell proliferation and NK cell activity were 103, 126 and 22.2% in group treated with IGF-I rich fraction $1{\mu}g/mL$ to compared to the control, respectively.
Keywords
IGF-I; macrophage; splenocyte; immune response;
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1 Battelli, M. G., Abbondanza, A., Musiani, S., Buonamici, L., Stroccm, P., Tazzari, P. L., Gramantieri, L., and Stirpe, F. (1999) Determination of xanthine oxidase in human serum by a competitive enzyme-linked immunosorbent assay(ELISA). Clin. Chim. Acta. 281, 147-158   DOI   ScienceOn
2 Green, L. C., Wagner, D. A., Glogowski, J., Skipper, P. L., Wishnok, J. S. and Tannenbaum, S. R. (1982) Analy­sis of nitrate, nitrite and [$^{15}N$] nitrate in biological fluids. Anal. Biochem. 126, 131-138   DOI   ScienceOn
3 Keller, R., Geiges, M., and Keist, R. (1990) L-arginine­dependent reactive nitrogen intermediates as mediators of tumor cell killing by activated macrophages. Cancer Res. 50, 1421-1425
4 Nathan, C. F. and Root, R. K. (1977) Hydrogen peroxide release from mouse peritoneal macrophages: dependen­ceon sequential activation and triggering. J. Exp. Med. 146. 1648-1662   DOI   ScienceOn
5 Pakkanen, R. and Aalto, J. 1997. Growth factors and antimicrobial factors of bovine colostrum. Int. Dairy J. 7, 285-297   DOI   ScienceOn
6 Regester, G. O., Smithers, G. W., Mitchell, I. R., McIn­tosh, G. H., and Dionysius, D. A. (1997) Bioactive factors in milk: Natural and induced. In: Milk composition, production and biotechnology. Welch, R. A. S., Bums, D. J. W., Davis, S. R., Popay, A. I., and Prosser, C. G. (eds.), CAB International. NY, pp. 119-132
7 Simmen, F. A., Simmen, R. C. M., and Reihnart, G. (1988) Maternal and neonatal somatomedin-C/insulin-like growth factor-I(IGF-I) and IGF binding proteins during early lactation in the pig. Dev. Biol. 130, 16-27   DOI   ScienceOn
8 Holsapple, M. P., Tucker, A. N., McNerney, P. J., and White, K. L. Jr. (1984) Effects of N-nitroso-dimetylamine on humoral immunity. J. Pharmacal. Exp. Ther. 229, 493-500
9 Okimura, T., Ogawa, M., and Yamauchi, T. (1986) Stress and immune responses. III. Effect of restraint stress on delayed type hypersensitivity(DTH) response, natural killer(NK) activity and phagocytosis in mice. Jpn. J. Phamacol. 41, 229-235
10 Wilson, J. (1997) Immune system breakthrough: Colos­trum. J. Longevity Res. 3, 7-10
11 Hossner, K. L. and Yemm, R. S. (2000) Improved reco­very of insulin-like growth factors(IGFs) from bovine colostrum using alkaline diafiltration. Biotechnol. Appl. Biochem. 32, 161-166   DOI   ScienceOn
12 Ortaldo, J. R. and Hernerman, R. B. (1984) Heterogeneity of natural killer cells. Annu. Rev. Immunol. 2, 359-394   DOI   ScienceOn
13 Trinchieri, G. (1989) Biology of natural killer cells. Adv. Immunol. 47, 187-376   DOI
14 Landreth, K. S., Narayanan, R., and Dorshkind, K. (1992) Insulin-like growth factor-I regulates pro-B cell differen­tiation. Blood. 80, 1207-1212
15 Ding, A. H., Nathan, C. F., and Stuher, D. J. 1988. Release of reactive oxygen intermediates from mouse peritoneal macrophage. Composition of activating cytoki­nes and evidences for independent production. J. Immu­nol. 141, 2407-2412
16 Hossenlopp, P., Seurin, D., Segovia-Quinson, B., Har­douin, S., and Binoux, M. (1986) Analysis of serum insulin-like growth factor binding protein using western blotting: use of the method for titration of the binding proteins and competitive binding studies. Anal. Biochem. 154, 138-143   DOI   ScienceOn
17 Riches, D. W., Chan, E. D., and Winston, B. W. (1996) TNF-alpha-induced regulation and signalling in macropha­ges. Immunobiology. 195, 477-90   DOI
18 Flick, D. A. and Grifford, G. E. (1984) Comparison of in vitro cell cytotoxic assay for tumor necrosis factor. J. Immunol. Methods. 68, 167-175   DOI   ScienceOn
19 Kashyap, S. R., Roman, L. J., Lamont, J., Masters, B. S., Bajaj, M., Suraamomkul, S., Belfort, R., Bema, R., Kel­logg, D. L. Jr., Liu, Y., and DeFronzo, R. A. (2005) Insulin resistance is associated with impaired nitric oxide synthase activity in skeletal muscle of type 2 diabetic subjects. J. Clin. Endocrinol. Metab. 90, 1100-1105   DOI
20 Klimetzek, V. and Remold, H. G. (1980) The murine bone marrow macrophage, a sensitive indicator cell for murine migration inhibitory factor and a new method for their harvest. Cell. Immunol. 53, 257-266   DOI   ScienceOn
21 Schams, D. (1994) Growth factors in milk. Endocr. Regul. 28, 3-8
22 Butler, J. E. (1994) Passive immunity and immuno­globulin diversity. In Proceedings of the IDF seminar: Indigenous antimicrobal agents of milk-recent develop­ments. Brussels: International Dairy Federation. 14-50
23 Stossel, T. P. (1973) Evaluation of opsonic and leukocyte function with a spectrophotomeric test in patients with infection and with phagocytic disorders. Blood. 42, 121-­130
24 Clark, R., Strasser, J., McCabe, S., Robbins, K., and Jardieu, P. (1993) Insulin-like growth factor-I stimulation of lymphopoiesis. J. Clin. Invest. 92, 540-548   DOI   ScienceOn
25 Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227, 680-685   DOI   ScienceOn
26 Heemskerk, M. H., Hooijberg, E., Ruizendaal, J. J., van der Weide, M. M., Kueter, E., Bakker, A. Q., Schu­macher, T. N., and Spits, H. (1999) Enrichment of an antigen-specific T cell response by retrovirally transduced human dendritic cells. Cell. Immunol. 195, 10-17   DOI   ScienceOn
27 Muraguchi, A., Hirano, T., Tang, B., Matsuda, T., Horii, Y., Nakajima, K., and Kishimoto, T. (1988) The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells. J. Exp. Med. 167, 332-344   DOI
28 Hagiwara, K., Kataoka, S., Yamanaka, H., Kirisawa. R., and Iwai, H. (2000) Detection of cytokines iri bovine colostrum. Vet. Immunol. Immunopathol. 76, 183-90   DOI   ScienceOn