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Extraction of Short Peptide Using Supported Liquid Membranes  

Lee, Jae-Heung (Department of Food Science and Biotechnology, Sungkyunkwan University)
Park, Ki-Moon (Department of Food Science and Biotechnology, Sungkyunkwan University)
Publication Information
Food Science of Animal Resources / v.25, no.3, 2005 , pp. 340-343 More about this Journal
Abstract
The objective of this work was to study separation of short peptide (glycine-tyrosine) by using supported liquid membranes (SLMs) containing Aliquat as a cationic carrier, In the present investigation, the influence of pH of donor phase, concentrations of carrier and salt concentrations of acceptor phase on separation flux rate were investigated. Below pH 7.0 the flux rate was not affected by NaCl concentration or carrier concentration. However, the rate was increased significantly above pH 7.0. The rate with Hossain's SLM(H-SLM) containing $20\%$ Aliquat was about 3-fold higher with pH 9.0 at 0.25 M NaCl and 10-fold higher with pH 8.0 at 1.0 M NaCl than that with Duggan's SLM(D-SLM) containing $8\%$ Aliquat respectively. Furthermore, the rate with H-SLM was 10-fold higher at 1.0 M NaCl than the rate with 0.25 M NaCl, In conclusion, it would appear that the rate of separation was facilitated by using high salt concentrations together with high carrier concentrations above pH 7.0.
Keywords
short peptide; supported liquid membrane; Aliquat;
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