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Effect of RFLP Marker of the Mitochondrial DNA D-Loop Region on Milk Production in Korean Cattle  

Chung Eui-Ryong (Department of Biotechnology, College of Life Science and Natural Resources, Sangji University)
Chung Ku-Young (Department of Animal Science and Technology, Sangji University)
Publication Information
Food Science of Animal Resources / v.25, no.2, 2005 , pp. 218-225 More about this Journal
Abstract
This study was performed to determine sequence variation and RFLP of the mt DNA D-loop region using Southern blot hybridization analysis and to develop mt DNA marker affecting milk production traits in Hanwoo cows. The PCR was used to amplify an 1142 bp fragment within the D-loop region of mt DNA using specific primers. Mt DNA were digested with seven restriction enzymes and hybridized using DIG-labeled D-loop probe. The mt DNA RFLP polymorphisms were observed in the four enzymes, BamHI, RsaI, XbaI and HpaII. Nucleotide substitutions were detected at positions 441 (G/C), 469 (T/C), 503 (C/T), 569 (G/A), 614 (C/A) and 644 (C/T) of the mt DNA D-loop region between two selected lines. Significant relationship between the XbaI RFLP type and breeding value was found(p<0.05). Cows with A type had higher estimated breeding values than those with B type (P<0.05) between high and low milk production lines. Therefore, the RFLP marker of mt DNA could be used as a selection assisted tool for individuals with high milk producing ability in Hanwoo.
Keywords
mt DNA; RFLP marker; nucleotide sequence; milk production; Hanwoo cow;
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