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Feasibility Study on the Use of Liposomes for Detecting Food-borne Pathogenic Bacteria  

김명희 (한국식품개발연구원)
김왕준 (한국식품개발연구원)
신원선 (한국식품개발연구원)
손동화 (한국식품개발연구원)
차성관 (한국식품개발연구원)
Publication Information
Food Science of Animal Resources / v.23, no.3, 2003 , pp. 278-283 More about this Journal
Abstract
Feasibility tests on using liposomes for detecting food-borne pathogenic bacteria were studied with E. coli 0157:H7 as a model analyte. lmmunoliposomes, whose surface was conjugated with anti-E. coli 0157:H7 IgG and which encapsulated the marker dye, sulforhodamine B, were used for the detection label. Among the feasibility tests, the first test was to use a test-strip on which antibodies to anti-E. coli O157:H7 IgG were immobilized. In this format, immunoliposomes that did not bind to E. coli O157:H7 in sample were captured and then exhibited a visible signal which was inversely related with the number of E. coli O157:H7 in sample. The second test was a direct liposome assay followed by immunomagnetic separation. In this format, immunoliposomes which were bound to E. coli O157:H7 were lysed with detergent and produced a signal which was proportionally related with the number of E. coli O157:H7 in sample. The results from both formats indicate that liposomes can be utilized as a detection label.
Keywords
liposome; test-strip; immunomagnetic bead; E. coli O157:H7; immunoassay; safety;
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