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Safety Evaluation of IGEs Separated and Refined from Colostrum  

조양희 (한국보건산업진흥원)
이수원 (성균관대학교 식품생명자원학과)
정명섭 (한국보건산업진흥원)
백승화 (충북과학대학 식품생명과학과)
제갈승주 (원광보건대학 임상병리과)
박강용 (한국보건산업진흥원)
Publication Information
Food Science of Animal Resources / v.23, no.2, 2003 , pp. 137-144 More about this Journal
Abstract
This study was carried out to investigate safety evaluation of IGEs separated and refined from bovine milk and commercial recombinant human IGFs. In order to evaluate toxicity of these samples, acute toxicity test and short term toxicity test were investigated with IGF-I separated and refined from colostrum and commercial recombinant human IGF-I from R&D systems company. for acute toxicity test, we selected recombinant human IGF-I from R&D systems company and establish one control group and three dose-level groups(0, 10, 20 and 50 $\mu\textrm{g}$ per rat). We have intravenously injected tail of rats with selected sample once. After 20 days, pathological cellular tissue analyses were investigated with liver, kidney and spleen of 12 rats in all test groups. However, Morbid tissue and abnormal statistical results were not discovered in all cellular tissues. For short term toxicity test, we selected IGF-I separated and refined from colostrum and establish one control group and three dose-level groups(0, 5, 10 and 15 $\mu\textrm{g}$/day per rat). Rats were orally injected with selected sample once a day during two weeks. After short term toxicity test period, Pathological cellular tissue analyses were investigate with liver, kidney and spleen of 12 rats in all test groups. However, Morbid tissue and abnormal statistical results were not discovered in all cellular tissues. These results suggest that IGF-I treated groups show no significant toxicological findings with changes of body weight, food consumption, water consumption, and pathological findings compared with control groups.
Keywords
IGFs; Acute toxicity; Short term toxicity; Pathological cellular tissue analyses;
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