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http://dx.doi.org/10.17642/jcc.23.4.9

Anti-allergic Effect of Eckolona cava Ethyl Acetate Fraction of on IgE/BSA-stimulated Bone Marrow-derived Cultured Mast Cells  

Han, Eui Jeong (Department of Food Technology and Nutrition, Chonnam National University)
Kim, Hyun Soo (Department of Marine Life Science, Jeju National University)
Shin, Eun Ji (Department of Food Technology and Nutrition, Chonnam National University)
Kim, Min Ju (Department of Food Technology and Nutrition, Chonnam National University)
Han, Hee-Jin (Department of Food Technology and Nutrition, Chonnam National University)
Jeon, You-Jin (Department of Marine Life Science, Jeju National University)
Jee, Youngheun (Department of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University)
Ahn, Ginnae (Department of Food Technology and Nutrition, Chonnam National University)
Publication Information
Journal of Chitin and Chitosan / v.23, no.4, 2018 , pp. 277-284 More about this Journal
Abstract
In this study, we investigated the anti-allergic effect of the ethyl acetate fraction of Ecklonia cava (EC-EtoAc) on the immunoglobulin E (IgE)/bovine serum albumin (BSA)-mediated activation of bone marrow-derived cultured mast cells (BMCMCs). We revealed that the $62.5{\mu}g/ml$ of EC-fractions ($EC-CHCl_3$, EC-Hexane and EC-EtoAc) inhibited IgE/BSA-activated ${\beta}$-hexosaminidase release from BMCMCs without cytotoxicity. Especially, EC-EtoAc showed the higher ${\beta}$-hexosaminidase release than the others. Also, EC-EtoAc reduced the expression levels of cytokines such as interleukin (IL)-$1{\beta}$, IL-4, IL-5, IL-6, IL-10, IL-13, interferon (IFN)-${\gamma}$ and tumor necrosis factor (TNF)-${\alpha}$ and a chemokine, thymus- and activation-regulated chemokine (TARC), compared to the only IgE/BSA-treated BMCMCs. Furthermore, EC-EtoAc significantly prevented the binding of IgE to Fc epsilon receptor $(Fc{\varepsilon}R)I$ and reduced the $Fc{\varepsilon}RI$ expression on the sensitized BMCMCs. Taken together, these results suggest that E. cava may be the natural agent with beneficial potentials for the treatment of type I allergic diseases induced by mast cell activation.
Keywords
Ecklonia cava; Ethyl acetate fraction; Anti-allergic effect; BMCMCs;
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