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http://dx.doi.org/10.14480/JM.2021.19.4.261

Antioxidant and inflammation inhibitory effects from fruiting body extracts of Ganoderma applanatum  

Im, Kyung Hoan (Division of Life Sciences, Incheon National University)
Choi, Jaehyuk (Division of Life Sciences, Incheon National University)
Baek, Seung A (Honam National Institute of Biological Resources)
Lee, Tae Soo (Division of Life Sciences, Incheon National University)
Publication Information
Journal of Mushroom / v.19, no.4, 2021 , pp. 261-271 More about this Journal
Abstract
In this study, the antioxidant and anti-inflammatory effects of methanol extract (ME) and hot water extracts (HE) from the fruiting bodies of Ganoderma applanatum were investigated. The 1,1-diphenyl-2-picryl-hydrazy (DPPH) radical scavenging activity of 2.0 mg/mL ME (94.83%) was comparable to that of butylated hydroxytoluene (96.97%), the reference standard. The hydroxyl radical scavenging activities of ME and HE were similar to that of BHT at 2.0 mg/mL, whereas lipid peroxidation activity of the ME and HE were significantly lower than that of BHT. High-performance liquid chromatography analysis showed that the G. applanatum fruiting bodies contained nine phenolic compounds, which might contribute to antioxidant and anti-inflammatory activities. The survival rate of RAW 264.7 macrophages treated with 2.0 mg/mL ME and HE were 65.23 to 68.12% at 2.0 mg/mL, thereby indicating that the extracts were slightly cytotoxic at the concentration tested. The extracts also inhibited the nitric oxide (NO)-mediated expression of inducible nitric oxide synthase (iNOS) protein in lipopolysaccharide-induced RAW 264.7 macrophages and carrageenan-induced paw edema in rats. The study results demonstrated that the fruiting bodies of G. applanatum possessed good antioxidant and anti-inflammatory activities, which might be used to develop novel anti-inflammatory agents.
Keywords
Antioxidant; Anti-inflammatory; Edema; Ganoderma applanatum; Phenolic compound;
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