Browse > Article
http://dx.doi.org/10.14480/JM.2018.16.2.96

Mycelial growth characteristics of Sparassis latifolia according to liquid media and incubation conditions  

Lee, Yun-Hae (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services)
Gwon, Hee-Min (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services)
Gu, Ok (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services)
Choi, Jong-In (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services)
Jeon, Dae-Hoon (Mushroom Research Institute, Gyeonggido Agricultural Research & Extension Services)
Publication Information
Journal of Mushroom / v.16, no.2, 2018 , pp. 96-102 More about this Journal
Abstract
Sparassis latifolia is one of the most expensive mushrooms in Korean market owing to its high ${\beta}$-glucan content and immunoactivity. However, because of the long cultivation period and high contamination rates, it has low production efficiency. Therefore, we first need to establish the optimum conditions for liquid spawn production to increase its production efficiency. As a result of experiments, molasses culture medium was selected for mycelial growth. Also, the optimum sugar content for molasses and amount of aeration used were approximately 8 Brix% and 0.3~0.6 vvm, respectively. Mycelial dry weight increases, while the medium decreases, as the incubation period increases. Therefore, to achieve maximum production efficiency, the incubation period of 9 to 11 days is appropriate.
Keywords
Aeration; Incubation period; Liquid spawn; Sparassis latifolia;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
연도 인용수 순위
1 Oh DS, Park H, Park HS, Kim MS. Chae JK. 2006. Cultivation of cauliflower mushroom (Sparassis crispa) by use of coniferous sawdust-based media with wheat flour and molasses. J Mushroom Sci Prod. 4:39-42(in Korean).
2 Ohno, N. Harada, T. Masuzawa, S. Miura NN. Adachi, Y. Nakajima, M. Yadomae T. 2002. Antitumor activity and hematopoietic response of a ${\beta}$-glucan extracted from an edible mushroom Sparassis crispa Wulf.: Fr.(Aphyllophoromycetideae). Inter J Med Mushrooms 4:13-26.   DOI
3 Ohno, N. Nameda, S. Harada, T. Miura, NN. Adachi, Y. Nakajima, M. Yoshida, K. Yoshida H. Yadomae T. 2003. Immunomodulating activity of a ${\beta}$-glucan preparation, SCG, extracted from a culinary-medicinal mushroom Sparassis crispa Wulf.: Fr.(Aphyllophoromycetideae), and application to cancer patients. Inter J Med Mushrooms 5:359-368.
4 Park H, Lee BH, Ka KH, B WC, Oh DS, Park JM, Chun WJ. 2006. Cultivation of cauliflower mushroom(Sparassis crispa) by use of steam-treated coniferous sawdust. Mokchae Konghak 34:84-89(in Korean).
5 Ryu SR, Ka KH, Park H, Bak WC, Lee BH. 2009. Cultivation characteristics of Sparassis crispa strains using sawdust medium of Larix Kaempferi. J Kor Mycol. 37:49-54(in Korean).   DOI
6 Seo SY, Yoo YJ, Jeong GT, Ryu J, Ko BR, Choi JS, Kim MK. 2005. Optimal condition for mycelial growth of Sparassis crispa. J Mushroom Sci Prod. 3:45-51(in Korean).
7 Shim KK, Yoo YJ, Koo CD, Kim MK. 2014. Changes of nutrients in media and mycelia on liquid spawn culture of Lentinula edodes. J Mushroom Sci Prod. 42:144-149(in Korean).
8 Shim KK, Yoo YJ, Koo CD, Kim YS, Kim MK. 2012. Changes in the $CO_2$ and amount of mycelium growth of the liquid spawn on Flammulina velutipes. J Mushroom Sci Prod. 10:3-8(in Korean).
9 Statics of agriculture, Food and Rural Affarirs. 2017. http://library.mafra.go.kr(in Korean).
10 Yoo YJ, Seo SY, Seo KW, Choi DC, Jo HK, Yu YB, Soung YJ, Ryu J. 2010. Technical development for the short-log bag cultivation of Sparassis crispa. J Mushroom Sci Prod. 8:16-21(in Korean).
11 Jeong JS, Yoo YJ, Seo SY, Yu YB. 2011. Selection of suitable conditions of mycelial growth and materials of bag cultivation on Sparassis crispa. J Mushroom Sci Prod. 9:80-83(in Korean).
12 Cheong JC, Park JS, Hong IP, Seok SJ, Jhune CS, Lee CJ. 2008. Cultural characterfistics of cauliflower mushroom, Sparassis crispa. Kor J Mycol. 36:16-21(in Korean).   DOI
13 Choi MH, Han HK, Lee YJ, Jo HG, Shin HJ. 2014. In vitro anticancer activity of hydrophobic fractions of Sparassis latifolia extract using AGS, A529, and HepG2 cell lines. J Mushrooms. 12:304-310.(in Korean).   DOI
14 Jang MJ. 2009. Studies on development of Lentinus lepideus cultivation. Research Report of Gyeonggido Agricultural Research and Extension Services. pp.672-694(in Korean).
15 Jo HG, Choi MH, Shin HJ. 2015. Preparation of fermentation broth of Sparassis latifolia containing soluble ${\beta}$-glucan using four Lactobacillus species. J Mushrooms 13:50-55(in Korean).   DOI
16 Kim HJ, Han SK. 2008. Mushrooms of Gwangneung Forest. Korean National Arboretum. p361(in Korean).
17 Oh DS. 2009. Studies on site conditions of Sparassis crispa at it's natural habitat, artificial cultivation characteristics and utilization. Doctoral thesis of Graduate School Chonnam University(in Korean).