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http://dx.doi.org/10.14480/JM.2015.13.4.305

Isolation and Characterization of Bacillus subtilis CA105 from Spent Mushroom (Pleurotus ostreatus) Substrates  

Kim, Hye Soo (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Kim, Chul Hwan (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Kwon, Hyun Sook (Korea Promotion Institute for Traditional Medicine Industry)
Lee, Chan-Jung (Mushroom Research Division, International Institute of Horticultural & Herhal Science, RDA)
Kong, Won-Sik (Mushroom Research Division, International Institute of Horticultural & Herhal Science, RDA)
Cho, Soo Jeong (Dept. of Pharmaceutical Engineering, Gyeongnam National University of Science and Technology)
Publication Information
Journal of Mushroom / v.13, no.4, 2015 , pp. 305-309 More about this Journal
Abstract
In order to isolate compost-promoting bacteria with high activity of cellulase and xylanase, spent mushroom substrates with sawdust were collected from mushroom cultivation farm, Jinju, Gyeongnam in Korea. Among of the isolates, one strain, designated CA105 was selected by agar diffusion method. The strain CA105 was identified as members of the Bacillus subtilis by biochemical characteristics using VITEK 2 system. Comparative 16S rRNA gene sequence analysis showed that isolate CA105 formed a distinct phylogenetic tree within the genus Bacillus and was most closely related to Bacillus subtilis with 16S rRNA gene sequence similarity of 98.9%. On the basis of its physiological properties, biochemical characteristics and phylogenetic distinctiveness, isolate CA105 was classified within the genus Bacillus subtilis, for which the name Bacillus subtilis CA105 is proposed. The cellulase and xylanase activity of B. subtilis CA105 was slightly increased according to bacterial population from exponential phase to stationary phase in growth curve for Bacillus sp. CA105.
Keywords
Bacillus subtilis CA105; Cellulase; Compost-promoting bacteria; Spent mushroom substrates; Xylanase;
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1 Han HS, Woo S, Kim DK, Heo BG, Lee KD. 2010. Effects of composts on the growth, yield and effective components of Turmeric (Curcuma longa L.). Korean J Environ Agri. 29:138-145.   DOI
2 Hong JH, Park KJ. 2009. Composting characteristics of food waste-poultry manure mixture inoculated with effective microorganisms. J Lives Hous Env. 15:59-68.
3 Jeong JY, Jung KY, Nam SS. 1999. Evaluation of qualities with or without microbial inoculation for food waste composting. Korean J Environ Agri. 18:280-286.
4 Kim DJ, Shin HJ, Min BH, Yoon KH. 1995. Isolation of a Thermophilic Bacillus sp. producing the thermostable cellulase-free xylanase, and properties of the enzyme. Kor J Appl Microbial biotechnol. 23:304-310.
5 Kim JY, Heo SH, Hong JH. 2004. Isolation and characterization of an alkaline cellulase produced by alkalophilic Bacillus sp. HSH-810. Kor J Appl Microbial biotechnol. 40:139-146.
6 Kim TI, Han JD, Jeon BS, Ha SW, Yang CB, Kim MK. 1999. Isolation and characterization of bacillus subtilis CH-10 secreting cellulase from catttle manure. J Microbial. 35:277-282.
7 Lee JH, Choi SH. 2006. Xylanase production by Bacillus sp. A-6 isolated from rice bran. J Microbiol Biotechnol. 16:1856-1861.
8 Miller GL, Blum R, Glennon WE, Burton AL. 1960. Measurement of carboxymethyl cellulase activity. Ana Biochem. 2:127-132.
9 Nakano MM, Marahiel MA, Zuber P. 1988. Identification of a genetic locus required for biosynthesis of the lipopeptide antibiotic surfactin in Bacillus subtilis. J Bacteriol. 170:5662-5668.   DOI
10 Regine MD, Ptak M, Peypoux F, Michel G. 1985. Pore-forming properties of iturin A: a lipopeptide antibiotic. Biochim Biophys Acta. 815:405-409.   DOI
11 Roongsawang T, Kameyama T, Haruki M, Morikawa M. 2002. Isolation and characterization of a halotolerant Bacillus subtilis BBK-1 which produces three kinds of lipopeptides: bacillomycin L, plipastain and surfactin. Extremophiles 6:499-506.   DOI
12 Schallmey M, Singh A, Ward OP. 2004. Developments in the use of Bacillus species for industrial production. Can J Microbiol. 50:1-17.   DOI
13 Seki T, Chung CK, Mikami H, Oshima Y. 1978. Deoxyribonucleic acid homology and taxonomy of the genus Bacillus. Int J Syst Bacteriol. 28:182-189.   DOI
14 Shin PG, Cho SJ. 2011. Cellulase and Xylanase Activity of Compost-promoting Bacteria Bacillus sp. SJ21. Korean J Soil Sci Fert. 44:836-840.   DOI
15 Tchobanoglous G, Theisen H, Vigil SA. 1993. Integrated solid waste management, p. 689-691 In: Engineering, principles and management issues. McGraw-Hill Inter- national Editions.
16 Vanittanakam N, Loeffler W. 1986. Fengycin-a novel antifungal lipopeptide antibiotics produced by Bacillus subtilis F29-3. J Antibio Tokyo 39:888-901.   DOI
17 Williams BC, McMullan JT, McCahey S. 2001. An initial assessment of spent mushroom compost as apotential energy feedstock. Biores Technol. 79:227-230.   DOI