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http://dx.doi.org/10.14480/JM.2014.12.4.304

In vitro anti-cancer activity of hydrophobic fractions of Sparassis latifolia extract using AGS, A529, and HepG2 cell lines  

Choi, Moon-Hee (Department of Chemical Engineering, Graduate School of Engineering, Chosun University)
Han, Hyo-Kyung (College of Pharmacy, Dongguk University)
Lee, Yong-Jo (Department of Chemical Engineering, Graduate School of Engineering, Chosun University)
Jo, Han-Gyo (Department of Chemical Engineering, Graduate School of Engineering, Chosun University)
Shin, Hyun-Jae (Department of Chemical Engineering, Graduate School of Engineering, Chosun University)
Publication Information
Journal of Mushroom / v.12, no.4, 2014 , pp. 304-310 More about this Journal
Abstract
The use of mushrooms has immense potential in many diverse applications. Until now, more than 3,000 species are consumed around the world, and more than 100 have shown promising clinical activity against cancer and other chronic diseases. Sparassis latifolia (formerly S. crispa) is an edible mushroom that harbors ${\beta}$-glucan reported to possess immunostimulatory and anticancer properties. However there have been no reports on the anticancer activity of hydrophobic fractions of S. latifolia. In this study, the anticancer activities of S. latifolia extract and hydrophobic fractions were investigated using AGS (stomach cancer), A529 (lung cancer), and HepG2 (liver cancer) cell lines. In cytotoxicity results of A529 cells, fractions of A2, A3, A4, A6, A7, A8, A9, and A10 in all 12 fractions show low $IC_{50}$ values. For HepG2 cells, A7 fraction results in the lowest $IC_{50}$ value while A7, A8, and A11 fractions show low $IC_{50}$ values in AGS cells. S. latifolia extract lead to low cell viability in cancer cells, compared to positive control of paclitaxel. A compound with molecular weight of 181 were detected using HPLC-MS but not identified yet. As a result, the hydrophobic fractions of S. latifolia EtOH extract would be a possible candidate as natural anticancer agents in the future.
Keywords
Sparassis latifolia; ${\beta}$-glucan; $IC_{50}$; Anticancer activity; Paclitaxel; Hydrophobic fractions;
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1 Adachi Y, Okazaki M, Ohno N, Yadomae T. 1994. Enhancement of cytokine production by macrophages stimulated with ($1{\rightarrow}3$)-beta-D-glucan, grifolan (GRN), isolated from Grifola frondosa. Bio Pharm Bull. 17(12): 1554-1560.   DOI   ScienceOn
2 An SJ, Pae HO, Oh GS, Choi BM, Jeong S, Jang SI, Oh H, Kwon TO, Song CE, Chung HT. 2002. Inhibition of TNF-${\alpha}$, IL-$1{\beta}$, and IL-6 productions and NF-${\kappa}B$ activation in lipopolysaccharide-activated RAW 264.7 macrophages by catalposide, an iridoid glycoside isolated from Catalpa ovata G. Don (Bignoniaceae). Int Immunopharmacol. 2(8): 1173-1181.   DOI   ScienceOn
3 Bagchi D, Garg A, Krohn RL, Bagchi M, Tran MX, Stohs SJ. 1997. Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro. Res Comm Mol Pathol Pharmacol. 95(2): 179-189.
4 Chandrasekaran G, Oh DS, Shin HJ. 2011. Properties and potential applications of the culinary-medicinal cauliflower mushroom, Sparassis crispa Wulf.:Fr. (Aphyllophooromycetideae): A review. Int J Med Mushroom 13(2): 177-183.   DOI
5 Chung KS, Lee WC. 2005. Antitumor immunoactivities of the combinations containing the hot water extract of Sparassis crispa. J Pharm Sci(C.N.U.). 20: 58-63.
6 Choi ES, Oh DS, Shin HJ. 2011. Study on mycelial growth and ${\beta}$-glucan content by adjusting the culture medium compositions. The Kor Soc Mushroom Sci. 15(1): 51-51.
7 Fryer MJ. 1992. The antioxidant effects of thylakoid Vitamin E (${\alpha}$-tocopherol). Plant Cell Environ. 15(4): 381-392.   DOI
8 Ferreira ICFR, Barros L, Abreu RMV. 2009. Antioxidants in Wild Mushrooms. Curr Med Chem. 16(12): 1543-1560.   DOI   ScienceOn
9 Hong F, Yan J, Baran JT, Allendorf DJ, Hansen RD, Ostroff GR, Xing PX, Cheung NKV, Ross GD. 2004. Mechanism by which orally administered ${\beta}$-1, 3-glucans enhance the tumoricidal activity of antitumor monoclonal antibodies in murine tumor models. J Immunol. 173(2): 797-806.   DOI
10 Han DS, Jeon SW, Kim HJ. 2009. Study on the Antioxidant and Anticancer Effect of Extract of Stamens of Nelumbo nucifera and Kaempferol. Kor J Herbology. 24(1): 23-33.
11 Kawagishi H, Hayashi K, Tokuyama S, Hashimoto N, Kimura T, Dombo M. 2007. Novel bioactive compound from the Sparassis crispa mushroom. Biosci Biotechnol Biochem. 71(7): 1804-1806.   DOI   ScienceOn
12 Kim MY, Seguin P, Ahn JK, Kim JJ, Chun SC, Kim EH, Seo SH, Kang EY, Kim SL, Park YJ. 2008. Phenolic compound concentration and antioxidant activities of edible and medicinal mushrooms from Korea. J Agr Food Chem. 56: 7265-7270.   DOI   ScienceOn
13 Kim HJ, Han DS, Jeon SW. 2009. Study on the antioxidant and anticancer effects of extract of stamens of Nelumbo nucifera and kaemferol. Kor J Herbol. 24(1): 23-33.
14 Oh DS, Wang SJ, Kim HS, Wi AJ, Yoon SY, Park HS, Park HH. 2014. Optimal medium compostion of cauliflower mushroom(Sparassis latifolia) cultivation using douglas fir wood chip and comparison of the fruiting body. J Kor Wood Sci Technol. 42(4): 428-438.   DOI   ScienceOn
15 Kim IK, Yun YC, Shin YC, Yoo J. 2013. Effect of Sparassis crispa Extracts on Immune Cell Activation and Tumor Growth Inhibition. J Life Sci. 23(8): 984-988.   DOI   ScienceOn
16 Lee SY, Lee YG, Byeon SE, Han S, Choi SS, Kim AR, Lee J, Lee SJ, Hong NS, Cho JY. 2010. Mitogen activated protein kinases are prime signalling enzymes in nitric oxide porduction induced by solubel ${\beta}$-glucan from Sparassis crispa. Arch Pharm Res 33(11): 1753-1769.   DOI   ScienceOn
17 Moradali MF, Mostafavi H, Ghods S, Hedjaroude GA. 2007. Immunomodulating and anticancer agents in the realm of macromycetes fungi (macrofungi). Int Immunopharmacol. 7(6): 701-724.   DOI   ScienceOn
18 Ohno N, Miura NN, Nakajima M, Yadomae T. 2000. Antitumor 1, 3-beta-glucan from cultured fruit body of Sparassis crispa. Biol Pharm Bull. 23(7): 866-872.   DOI   ScienceOn
19 Ohno N, Harada T, Masuzawa S, Miura NN, Adachi Y, Nakajima M, Yadomae T. 2002. Antitumor Activity and Hematopoietic Response of a ${\beta}$-Glucan Extracted from an Edible and Medicinal Mushroom Sparassis crispa Wulf.: Fr.(Aphyllophoromycetideae). Int J Med Mushrooms. 4(1).
20 Park H, Lee BH, Oh DS, Ka KH, Bak WC, LEE HJ. 2005. Cultivation of cauliflower mushroom (Sparassis crispa) using coniferous sawdust-based media with barley flours. J Kor For En. 24(2): 31-36.   과학기술학회마을
21 Trapnell BC, Whitsett JA. 2002. GM-CSF regulates pulmonary surfactant homeostasis and alveolar macrophagemediated innate host defense. Annu Rev Physiol. 64(1): 775-802.   DOI   ScienceOn
22 Puttaraju NG, Venkateshaiah SU, Dharmesh SM, Urs SM, Somasundaram R. 2006. Antioxidant activity of indigenous edible mushrooms. J Agric Food Chem. 54(26): 9764-9772.   DOI   ScienceOn
23 Ryu SR, Ka KH, Park H, Bak WC, Lee BH. 2009. Cultivation characteristics of Sparassis crispa strains using sawdust medium of Larix kaempferi. Kor J Mycol. 37(1): 49-54.   과학기술학회마을   DOI   ScienceOn