The purposes of this investigations were survey of the occurred bacterial diseases, development of new animal health drug, guidance to formers on the treatment and control methods of diseases. Some series of investigations have been carried out by microbiological, pathological and serological examinations. The results could be summarized as follows. 1. A total of 953 cases of outbreaked swine diseases have been diagnosed in Clinical pathology laboratories, Bayer Vet Res Institute during 8 years (from 1986 to 1993). The high incidence diseases were colibacillosis, pleuropneumonia, streptococcal infection and pasteurellosis in decreasing order. 2. Pleuropneumonia caused by Actinobacillus pleuropneumoniae was the most important respiratory diseases and pasteurellosis by Pasteurella multocide could be confirmed in several cases. 3. Actinobacillus pleuropneumoniae 50 strains were isolated and identified by biochemical and serological tests. In serotyping test, 22 isolated strains were serotype 5, 21 strains as serotype 2, each 2 strains as serotype 3 and 7 by the coagglutination test. 4. Colibacillosis and edema discase caused by Escherichia coli has been the most predominant outbreaked disease in this investigations. The 100 isolates of E coli strains were sensitive to amikacin, colistin, enrofloxacin, gentamycin and trimethoprim -sulfamethoxazole. 5. Swine erysipelas caused by Erysipelothrix rhusiopathiae was confirmed 25 cases as acute septicemic forms. Isolates of E rhusiopathiae were highly sensitive to ampicillin, cephalothin, enrofloxcin, penicillin and tetracycline. 6. The 49 cases of hemorrhagic and necrotic enteritis in piglets were observed and 13 strains of Clostridium perfringens could be isolated and confirmed by biological and serological test. Isolates of Clostridium perfringens type C were highly sensitive to ampicillin, cephalothin, enrofloxacin, penicillin and trimethoprim- sulfamethoxazole. 7. The 14 strains of Streptococcus suis type II could be isolated from meningitis of piplets. 8. Polyserositis caused by Haemophilus parasuis and salmonellosis were observed and confirmed. Also Corynebacterial infections and several parasitosis have been also observed in this investigations.

The hematologic values were measured from 64 healthy goats imported from Australia to obtain data about the hematologic values of Australian wild goat. In paper 32 goats imported in March called group 1, 32 goats imported in April called group 2. The result obtained are summarized as follows;, 1 Mean$\pm$SD values and ranges of red blood cell(RBC) were $17.8$\pm$3.0{\times}10^6/${mu}ell$ and 9.9-24.3{\times}10^6/${mu}ell$,$ of hemoglobin(Hb) content 13.8$\pm$1.3 g/㎗ and 10.0-16.1 g/㎗, of packed cell volume(PCV) 37.0$\pm$4.1% and 28.0-47.0%, of mean corpuscular volume(MCV) 21.3$\pm$3.3 fl and 16.4-37.2 fl, of mean corpuscular hemoglobin(MCH) 7.9$\pm$1.1 pg and 6.0-13.3 pg, of mean corpuscular hemoglobin concentration(MCHC) 37. Of 1.9% and 34.0-41.9%, of white blood cell(WBC) $28.6$\pm$9.4{times}10^3/${mu}ell$ and 11.6-49.8{times}10^3/${mu}ell$,$ of neutrophil 38.0$\pm$8.2% and 23.0-61.0%, of lymphocyte 54.0$\pm$8.4% and 32.0-73.0%, monocyte 3.1$\pm$1.3% and 1.0-7.0%, of eosinophil 4.0$\pm$1.9% and 1.0-10.0%, of basophil 0.1$\pm$0.2% and 0-1.0% respectively. 2. There was no significant differences between Group l and Group 2, respectively. 3. There was no significant differences between sexes. 4. Comparing with the values of Korean domestic goat, e values of Australian wild goat were disserent from the values of Korean domestic goat. 5. These hematologic values might be accepted as the hematologic values of Australian wild goat.

데마슘과 오수생물인 Exserohilum rostratum 진균이 피부염에 걸린 2세의 암말 피부 병변으로부터 분리되었다 이 암말은 다수의 작고, 인설로 덮힌 불규칙한 모양의 백회색 탈모 병변을 목과 등에 나타내었다. 감염경로는 확인되지 않았으나 Exserohilum rostratum은 자연 상태에서 오수생물로써 증식한다. 저자들의 지식으로써는 피부병에 걸린 어린 말에서 Exserohilum rostratum의 분리는 새로운 숙주를 기록하는 것이다. 다양한 동물의 질병에서 Exserohilum rostratum의 역할을 조사해야 될 것을 제시하였다.

Sector scanner which has a conical end is used to image through the intercostal space because heart is protected by the ribs. Cardiac data published all around the world were also obtained by sector scanner. Although scanners being used in every small animal practice and animal hospital at college in Korea include convex ape and linear type, linear type is not appropriate f3r cardiac scan because of a wide contact surface. The purpose of this study is to establish ultrasonographic images of normal cardiac structures by measuring shape, size of reflectable cardiac structure according to restraint position in scanning normal heart of the puppies with 6.5 MHz convex scanner(SonoAce 4500, Medison, Korea) used in our veterinary teaching hospital, Seoul national university. Seventeen male and female puppies considered having healthy hear by X-ray and clinical examination are used feom April to July 1994. Scanning point selection of probe head and the distinction of imaged cardiac structures were accomplished by necropsy and cardiac scanning performed through thoracotomy under general anesthesia. At 10 o'clock position of transducer(at an angle of 30$^{\circ}$ between imaginary line from elbow joint to 3rd sternum and probe head, 60$^{\circ}$ from body surface, 4th intercostal space of right thorax) with the marker of scanner toward the head of dogs right atrium, left atrium and left ventricle were observed in 2, 3, 4, 5 intercostal space(2cm from the sternum) of experimental dog positioned ventrodorsally under general anesthesia. Under these conditions, the numerical values of imaged diastolic hear are as follows : the distance from skin to apex(mean$\pm$S.D) 47.53$\pm$6.94mm, thickness of left ventricular wall 6.00$\pm$1.60mm, length of left ventricle 16.27$\pm$5.31mm, width of left ventricle 15,33$\pm$4.25mm, length of left atrium 12.33$\pm$3.82mm, width of left atrium 11. 33$\pm$3.94mm, length of right atrium 1.00$\pm$2.41mm, width of right atrium 11.21$\pm$2.76mm and the area of left ventricle 270.92$\pm$109.81mm$^2$, area of left atrium 98.00$\pm$41.08mm$^2$, area of right atrium 62.75$\pm$21.04mm$^2$.

This study is first report on dermatitis digitalis and dermatitis verrucosa, which were occurred in bovine claw, in the Republic of Korea. Pathologically, dermatitis digitalis was observed in the from of surperficial epidermitis on the skin at the caudal end of interdigital cleft with whitish epithelial bordel while dermatitis verrucosa in the form of wart-like proliferation of the skin midway between the bulbs and adjacent to the coronary band. Histological finding of dermatitis verrucosa were noticeable parakeratotic hyperkeratosis of the epidermis, acanthosis, spongiosis of prickle cell layer and intercellular bridges.

This experiment was carried out to investigate the renditions to maintain good post-thaw motility and viability of canine spermatozoa when the semen was frozen using methanol. The semen from two male dogs which had been proven to be fertile in the previous one year was treated with different compositions of semen diluent and was frozen at different freezing temperatures, When canine semen was frozen at-2$0^{\circ}C$, -6$0^{\circ}C$, or -8$0^{\circ}C$, the spermatozoa frozen and stored at -2$0^{\circ}C$ showed very low post-thaw motility and viability from day 2 to 7 and showed no viability since day 15 after freezing. The spermatozoa frozen and stored at -6$0^{\circ}C$ or -8$0^{\circ}C$ showed higher post-thaw motility and viability on day 2, 1, 15 and 30 after freezing than that frozen and stored at-2$0^{\circ}C$(p<0.01), with no difference between two groups. Among different composition groups of the semen diluents of control(tris + egg yolk + glycerol), egg yolk-free, 히ycerol-free, and tris-free, Prior to freezing, the egg yolk-free diluent showed significantly love. motility and viability than the other diluents(p<0.05). On each thawing day (from day 2 to 15 after freezing), control group showed considerably higher motility and viability than the other groups(p<0.01). The canine spermatozoa frozen and stored at -6$0^{\circ}C$ and -8$0^{\circ}C$ showed gradual decrease of motility from day 2 to 30 after freezing and the spermatozoa of these two groups thawed on day 30 showed considerably love. motility than those thawed on day 2 after freezing, respectively(p<0.01). These results indicate that the freezing temperature of either -6$0^{\circ}C$ or -8$0^{\circ}C$ can be applicable to the freezing method using methanol and also all of the components of the semen diluent including cryoprotectant, buffer and cold-shock buffer are very important to maintain motility and viability of canine spermatozoa in the freezing and thawing procedure.

This experiment was conducted to evaluate the feed value of Macol meal, which is one of the byproducts from the extraction of liquid syrup from barley grain, on lactating dairy cows. All of the lactating dairy cows feeding in eight dairy farms were used in feeding experiment and fed concentrates plus Macol meal 5Kg as wet basis. The milk yields and milk fit during the periods of 10 months were investigated and blood picture, blood chemistry and diseases occuring frequency before and after feeding Macol meal were analyzed. The results obtained were as follows ; 1. In the proximate compositions, the contents of moisture, crude protein, crude fat, crude fiber, crude as and nitrogen free extracts was 68.8%, 7.43%, 1.04%, 7.08%, 1.56% and 14.09% respectively. On the other hand, the contents of NDF, ADF and cellulose was 36.68%, 35.71% and 10.85%, as dry matter basis, respectively. 2. The daily milk yield per head was 22.84kg during two months before feeding Macol meal and 23.89 kg during the periods of ten months after feeding Macol meal. 3. The blood picture and blood chemistry were not affected by feeding Macol meal. 4. The diseases occuring frequency were not significantly affected by feeding Macol meal. from the results of this experiment it could be concluded that Macol meal can be used in lactating dairy cows rations without any adverse effects.

Serum $\alpha$-tocopherol was measured in cattle to evaluate normal range and to investigate the difference of $\alpha$-tocopherol levels between healthy cattle and diseased cattle. Seventy two heads of 1 year old beef Cattle have 429.9$\pm$77.2 $\mu\textrm{g}$/100ml of Serum $\alpha$-tocopherol. The Serum $\alpha$-tocopherol values of calves with diarrhea(8 heads), pneumonia(6 heads) and piroplasmosis(4 heads) were 87.1$\pm$19.2, 126.3$\pm$45.7 and 106.3$\pm$30.9$\mu\textrm{g}$/100ml, respectively. But that of calves in good health (S heads) was 357.t$\pm$68.4 $\mu\textrm{g}$/100m1. And the values of diseased calves are significantly lower an that of calves in good health(p<0.05). Seasonaly, serum $\alpha$-tocopherol levels of dairy Holstein cows were 529.9$\pm$ 120.3(March), 540.2$\pm$127.2(June), 566.9$\pm$149.5(September) and 550,0$\pm$ 125.4(December)$\mu\textrm{g}$/100m1, respectively. The values on autumn was the highest than that of othor seasons. Serum H-tocopherol level of cows with retained placenta was 262.2$\pm$40.6$\mu\textrm{g}$/100m1. And the level of retained placenta was significantly lower than that of healthy cattle regardless seasonal variation(p<0.05).

A 14-year-old virgin, female Yorkshire Terrier showed enlarged abdomen after udderectomy was diagnosed with canine mammary gland tumor accompanied with hydrometra. Gross findings appeared a white-yellowish $l0{\times}7{\times}7cm sized solid mass containing cysts filled with various colored mucoid fluids in the udder. Both uteri were distended greatly with white-yellowish watery fluids and their serosal surfaces were translucent. Characteristic micyoscopic findings in the mass of the udder showed great expansion of the mammary lobule and stroma and complete replacement of the glandular elements by prolifaration of myoepithelial cells. The mucosa of the uterus showed severe atrophy of the uterine epithelium.

A lymphosarcoma was diagnosed in a 4-year-old female Formosan sika deer presented with persistent reproductive failure, anorexia, depression and diarrhea. Characteristic pathological findings were infiltration of neoplastic lymphoid cells and cancer emboli in the lymph nodes, heart, lung, kidney, urinary bladder, ovary, uterus and peritoneal fat tissue.

In the present studies, samples of blood were taken from 24 Holstein cattle over 2 years old. By using the angled tube method the normal ESR values were determined in cattle under various diseases. The results obtained were summarized as follows ; The standardized ESR/hr using 45$^{\circ}$ angled capillary tubes were 19.0% in case of necrotic mastitis, 23.0% in nephritis, 12.2% in anemia and 4.2% in diarrhea. And the ESR was increased from some necrotic disease, decreased from diarrhea conditions and general the normal ESRs were shown in the sub-clinical or recovery stage of infections or disorders.

A consecutive Jiao Chio acupuncture therapy was performed for 3 days in the 45-90 days old 11 calves of which have been shown severe watery diarrhea. The discharge of the infected calves was yellowish brown in color. Two calves of these patients were infected wi pulmonary disease as well as diarrhea. Thus, Su Qi and Fei Yu acupuncture therapy was carried out additionally after dosing with antibiotics twice for The two infected calves. Blood chemical values and serum neutralizing antibody titers were checked, and total blood cell count was also carried out to know the therapeutic effect before and after(21 days) acupuncture therapeutics. The results are as follows ; 1, The diarrhea has ceased one day after begining of the acupuncture therapy in 5 calves, and the cessation of the diarrhea in remaining calves occurred in 1 calf each on 3rd and 4th day, and 2 calves on 6th day, respectively. Two calves infected with pulmonary disease as well diarrhea were cured 8 days after the begining of the therapcutics. 2. Rotaviruses wire detected in the feces of 2 calves, and bovine diarrhea viruses were detected in the 8 calves by the test for serum neutralizing antibody titers, and bovine coronaviruses were also detected in 5 calves. Four calves of the 5 bovine coronavirus infected calves were also infected with bovine diarrhea viruses. 3. Total leucocyte number, total amount of serum protein, and amount of fibringen were slightly increased, while total erythrocyte number, and erythrocyte packed cell volume were slightly decreased. These valucs were statistically not significant. Electrolytes of Na/sup +/, K/sup +/ and Cl/sup -/ were slightly decreased but these values also were not significant. These results indicate that the acupuncture therapeutics arc significantly effective to remove the viral diarrhea in the young calves.

본 연구의 목적은 초음파검사에 의하여 한국재래산양의 간장 및 담낭의 정상적인 구조와 크기를 알아보는데 있다. 30두의 한국재래산양에서 간장, 문맥, 후대정맥 및 담낭의 크기, 위치 및 구조를 측정하였다. 30마리의 산양을 체중에 따라서 5-15Kg, 15-25Kg 및 25-35Kg군으로 분류하였다. 우측복벽의 제9늑간극으로부터 12늑간극까지에서 측정을 실시하였다. 각 늑간극에서의 간장의 크기, 후대정맥 및 문맥의 위치 및 직경을 관찰하였다. 본 연구에 있어서의 간장 크기 및 위치의 초음파 측정자료는 한국재래산양의 간장의 변화에 대한 진단에 있어서 기초자료로서 사용될 수 있다는 결론을 얻었다.