In order to study the rate control step in the protein metabolic course of the chlorophyll formation, the transaminase activities which are obtained freely in the extracts of cotyledons of germinating peas at the light and the dark places, are measured in Beckman mopel D.U, spectrophoto meter at 490 mu. In this case, of two enzymatic reaction products; oxalacetic acid and pyruvic acid, the former is converted to pyruvic acid by aniline citrate and after each pyruvate phenyl hydrazones are extracted by toluenes: when this is treated with strong alcoholic alkali, a colored hydrazone is formed and it is measured by above apparatus. The estimated G.O.T. and G.P.T. in the germinated cotyledons at dark and light places considerably differ in their activities; G.O.T. and G.P.T. activities which are formed at the light are more increased than at the dark and also they differ in their rates through germination, though G.O.T. activity increment is smoothly but that of G.P.T. is more sharply, and they are considered to be directly affected to the chlorophyll formation and indirectly to the growth. G.O.T. and G.P.T. in each fractions of cell in the cotyledons should be formed by dissociation of zymogens in the microsomal fractions and it seems to promoted by light. In the formation of the chlorophyll, the protein metabolism occurred mainly in the microsomal fractions and the rate determining step is found at the point where the zymogene that is able to produce G.P.T. is activated, and this activation is promoted by light as noted above.

1) Rhizopus균(菌) 8주(株)와 Aspergillus속균(屬菌) 7주(株)의 당화효소생산력(糖化酵素生産力)(전분가수분해율(澱粉加水粉解率))을 비교한 결과 Rhizopus delemar ND 1과 Aspergillus usamii mut. shirousamii의 두 균주(菌株)를 각각 선정(選定)하였다. 2) Rhizopus delemar ND 1은 전분당화율(澱粉糖化率)이 높고 효소전이당(酵素轉移糖)의 생성(生成)이 적으므로 효소당화(酵素糖化) 포도당(葡萄糖)의 제조(製造)에 적합(適合)하고 Asp. usamii mut. shirousamii는 당화율(糖化率)이 약간(若干) 떨어지나 효소전이당(酵素轉移糖)의 생성(生成)이 인정(認定)되므로 특수(特殊)한 풍미(風味)를 필요(必要)로 하는 전분이(澱粉飴) 제조(製造)에 적합(適合)함을 알았다. 3) Rhizopus delemar ND 1의 당화효소(糖化酵素)를 시용(使用)한 예비시험(豫備試驗)에서 정제분말(精製粉末) 포도당(葡萄糖)을 얻을 수 있었다.

A study on fungus flora of 8 paddy field soil samples including 5 AKIOCHI soils has been made. Samples were taken from Chung-nam area. Fungi of 5 Genera and 19 species were identified; Aspergillus, Penicillium, Spicaria, Curvularia, Trichodema, were the 5 genera and the 19 speciec consristed of 4 Aspergilli, 12 Penicilli, and the each one of the others. Variations of fungus flora among those soil samples were noticed without the ecologieal explanation.

The results of re-examination for the isolates D-28, D-28a from Kimchi and 88-3 from laver are summarized as follows: 1). All these isolates were proved to be Gram positive, catalase positive and spore-forming. 2). While these strains were not consistent with Bacillus megaterium group with respect to Voges-Proskauer reaction and methyl red test, their cultural characteristics were well consistent with this group. 3). These isolates showed the similar suger-fermentability as B. megaterium group with a slight difference. 4). It is proved that, according to the forgoing results and vitamin $B_{12}$ producibility, the isolate 88-3 is identical with B. megaterium NRRL-B 938 and D-28, D-28a, with B. megaterium 425-1 and 425-2, respectively.

tragacanth gum의 화학구조(化學構造)를 구명(究明)하기 위(爲)하여 미국(美國) 약전(藥典)의 tragacanth gum 분말(粉末)을 가지고 다음의 실험(實驗)을 통(通)하여 이의 성분(成分)의 하나인 polysaccharide C를 분리(分離)하여 이의 화학구조(化學構造)를 밝혔다. (1) tragacanth gum을 85% 주정(酒精)으로 처리(處理)해서 중성다당류(中性多糖類)로 polysaccharide C를 분리(分離)하였으며 구성당(構成糖)으로 L-rhamnose, D-xylose, L-arabinose 및 D-galactose를 paper chromatography와 Cellulose column chromatography로 분리(分離), 동정(同定)하였다. 이의 molar ratio는 2:1:17:9이며 비선광도(比旋光度)는 $[{\alpha}]^{30}_D-72.2이다. (2) 구성당(構成糖)의 결합위치(結合位置)를 구명(究明)하기 위(爲)해 Hawarth 법(法)과 Purdietldir(試藥)을 가지고 methyl화(化)시켜 methyl화(化) polysaccharide C를 얻었으며 비선광도(比旋光度) $[{\alpha}]^{22}_D-102를 보였다. 이것을 가수분해(加水分解)시켜 paper chromatography와 column chromatography를 통(通)해 methyl화단당(化單糖)으로 1,3,5-tri-O-methyl-L-arabofuranose, 3,4-di-O-methyl-L-rhamnose, 2,3-di-O-methyl-D-xylose, 2,3,4-tri-O-methyl-D-galactopyranose, 2,4-di-O-methyl-L-arabopyronose, 2,4-di-O-methyl-D-galactose, 2-O-methyl-L-arabinose 및 L-arabinose를 분리(分離), 동정(同定)하였다. (3) 산(酸)의 각종농도(各種濃度)에 따른 부분적(部分的) 가수분해(加水分解)를 시켜 polysaccharide C의 end group, 측지(側枝) 또는 주쇄(主鎖)를 이루는 구성당(構成糖)을 밝히기 위(爲)하여 0.05 N-HCl로 제1차(第一次) 가수분해(加水分解). 0.1N-HCl로 제2차(第二次) 가수분해(加水分解), 0.3N-HCl로 제3차(第三次) 가수분해(加水分解)를 하여 가수분해물(加水分解物)과 비가수분해물(非加水分解物)에서 각각(各各) 다음과 같은 구성단당(構成單糖)을 검출(檢出)하고 이들의 molar ratio를 측정(測定)하였다. 제1차(第一次) 가수분해물(加水分解物)(A)에서 L-arbinose, 비가수분해물(非加水分解物)(A')에서 L-rhamnose, D-xylose, L-arabinose 및 D-galactose; 제2차(第二次) 가수분해물(加水分解物)(B)에서 L-arabinose와 D-galactose, 비가수분해물(非加水分解物)(B')에서 L-rhamnose, D-xylose, L-arabinose, 및 D-galactose; 제3차(第三次) 가수분해물(加水分解物)(C)에서 L-rhamnose, D-xylose, L-arabinose 및 D-galactose, 비가수분해물(非加水分解物)(C')에서 D-xylose와 D-galactose를 검출(檢出)하였다. (4) 구성당(構成糖)의 형태(形態)와 구조(構造)를 밝히기 위(爲)해 polysaccharide C에 대한 periodate산화(酸化) 실험(實驗)을 하여 $C_5H_8O_4$당(當) periodate의 소비(消費)와 formic acid의 생성량(生成量)을 측정(測定)하였는데 periodate의 소비량(消費量)은 1.23 mole, formic acid의 생성량(生成量)은 0.78 mole이다.