Hydrotreated biodiesel (HBD) would be one of the promising alternative fuels instead of current biodiesel. In this study, spray characteristics in terms of spray penetration and spray angle were conducted experimentally including calculated SMDs as well. The ambient pressures of 1, 3, and 5 MPa and injection pressures of 30, 80, and 130 MPa were introduced and the fuels employed were petro-diesel, and 2, 10, 20, 30, and 50% for hydrotreated biodiesel, respectively. The result of this study found that the more HBD blended diesels have the slightly shorter spray tip penetration lengths especially on the lowest injection pressure and at the highest ambient pressure, but have the larger spray angles and SMDs than petro-diesel. Consequently, this study found that HBD has a little bit merits and demerits of macro- and micro- spray patterns compared to petro-diesel.